LOCUS       Exported                6541 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2691
                     /codon_start=1
                     /label=Tigger3
                     /translation="MAPKRTKSTANVASKRPHRVTDLETKLKVIKDYEGGKSVMVIARQ
                     SGMSHSTIATILKNKNKVTEAVKGSASLKATRLTKIREGPISDMEKLLMTWIEDQTQKH
                     IPLSTMTITAKAKSLFAMLKEKAGPDYDVEFTASSGWFKRFKNRYSLHNVKVSGESASA
                     DVKAAEEFLETLDKLIVEENYLPEQIFNMDETSLFWKRMPERTFIHKEAKSMPGFKAFK
                     DRITVLLGGNVAGYKLKPFVIWHSENPRAFKHINKHTLPVYYRSNKKSWMTQLLFQDAL
                     LNCYASEMEKYCLENNIPFKILLIVDNAPAHPPFIGDLHPNIKVVFLPPNTTSLIQPMD
                     QGVIAAFKAYYLRRTFAQAIAATEEDTEKTLMQFWKDYNIYDCIKNLAWAWGDVTKECM
                     NGIWKKTLKRFVRDFKGFAKDEEVAKINKAVVEMANNFNLGVDEDDIEELLEVVPEELT
                     NEELLELEQERIAEEEAREKETAGEEKEEPPRKFTVKGLAEAFADLNKLLKKFENMDPN
                     TERFSLIERNVHGALSAYKQIYDEKKKQTKQTTMDIFLKRVTPPQEEPQAGPSGGIPEE
                     GIVIIGDDSSMRVIAPEDLPVGQDVEVEDSDIDDPDPV"
     promoter        complement(2729..2747)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2773..2997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3043..3471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3485..3814
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3665..3800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3980..4101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4150..4166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4174..4190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4198..4228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4243..4264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4552..5140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5311..6171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6172..6276)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg cacctaagcg taccaaaagc accgcgaacg ttgcaagcaa acgccctcat
      901 cgtgtgaccg atctggaaac caagctgaag gtgatcaaag attatgaagg cggcaaaagc
      961 gtgatggtga ttgcgcgtca gagcggcatg agccatagca ccatcgcgac cattctgaag
     1021 aacaagaaca aagtgaccga agccgtgaaa ggtagcgcaa gcctgaaagc gacccgcctg
     1081 accaaaatac gcgaaggccc gattagcgat atggagaagc tgctgatgac ctggatcgaa
     1141 gatcagaccc agaagcatat ccctctgagc accatgacca tcaccgccaa agcgaaaagc
     1201 ctgtttgcca tgctgaagga gaaagcgggc ccggattacg atgttgagtt caccgcgagc
     1261 agcggttggt tcaaacgctt caaaaaccgc tacagcctgc acaacgtgaa agtgagcggc
     1321 gaaagcgcaa gcgcagatgt taaagccgcc gaagaatttc tggaaaccct ggacaaactg
     1381 attgtggaag aaaactatct gccggagcag atttttaaca tggacgaaac cagcctgttc
     1441 tggaaacgta tgccggagcg cacctttatc cataaagaag ccaagagcat gccgggcttt
     1501 aaggcgttta aggaccgcat taccgtgctg ctgggtggta acgttgccgg ttacaagctg
     1561 aaaccgttcg tgatttggca tagcgaaaac ccgcgcgcgt tcaagcacat caacaagcac
     1621 accctgcctg tgtactatcg cagcaacaag aagagctgga tgacccagct gctgtttcag
     1681 gatgcgctgc tgaattgcta cgcgagcgag atggaaaaat attgcctgga aaacaacatc
     1741 ccgttcaaga ttctgctgat tgtggacaac gcgcctgcgc atcctccgtt tatcggcgat
     1801 ctgcacccta acattaaagt ggtgttcctg ccgcctaata ccaccagcct gattcagccg
     1861 atggatcagg gtgttattgc ggcgttcaag gcgtattatc tgcgccgcac ctttgcacaa
     1921 gcgattgcgg cgaccgaaga agacaccgaa aagaccctga tgcagttctg gaaggactac
     1981 aacatctacg attgcatcaa gaacctggcg tgggcatggg gtgacgtgac caaagaatgc
     2041 atgaacggca tttggaagaa aaccctgaaa cgctttgtgc gcgatttcaa aggctttgcg
     2101 aaagatgaag aagtggcgaa aatcaacaaa gcggtggtgg agatggcgaa caacttcaac
     2161 ctgggcgtgg atgaggatga tattgaagaa ctgctggaag tggtgcctga ggagctgacc
     2221 aacgaagagc tgctggaact ggaacaggag cgcattgcag aagaagaggc gcgcgaaaaa
     2281 gaaaccgcgg gcgaagagaa agaagaaccg ccgcgcaagt ttaccgttaa gggcctggcg
     2341 gaagcatttg ccgatctgaa caagctgctg aaaaagtttg agaacatgga cccgaacacc
     2401 gaacgcttta gcctgatcga gcgcaatgtg catggtgcac tgagcgcgta caaacagatt
     2461 tacgacgaga aaaagaaaca gaccaaacag accaccatgg acattttcct gaaacgcgtg
     2521 acccctccgc aagaagaacc tcaagcgggt ccttcaggtg gtataccgga agaaggcatt
     2581 gtgatcattg gcgacgacag cagcatgcgt gttatcgcgc ctgaggattt acctgttggc
     2641 caggatgttg aagtggaaga tagcgatatt gacgatccgg accctgtgta atgagcggcc
     2701 gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     2761 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2821 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2881 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2941 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3001 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3061 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3121 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3181 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3241 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3301 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3361 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3421 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3481 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3541 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3721 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3781 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgaaatga ccgaccaagc
     3841 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3901 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3961 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4081 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     4141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4981 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6301 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     6361 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     6421 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     6481 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     6541 t
//
