LOCUS       Exported                6283 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2433
                     /codon_start=1
                     /label=hAT-14_Crp
                     /translation="MSAFVSGSSRKCDTQRAEKLTELLCTMITEDMLPVSVVEGAGFRA
                     LMSFVEPEYHVPGRQTVTSQLERWYENCVNSLRERLGKADKVAFTTDCWTALNAESYMT
                     VTCHYIENWELRSAVLQTESMPERHTAENIAEKLNAAVERWGLEGRVLACVHDNASNVV
                     LANMPRYVAWESVNCFAHTLQLAINDGFSGSVDHVIAAASRLVAHFHHSTVAAKALERK
                     QTQLQAKQHHLIQSCKTHWNSAYEMFERLSEQRWPITAVLSDRSVTKVSDAQTLELQDE
                     QWQLIEDILPVLSTLKCATTAMSAEHSASISNIYPICNSLLNTHLKSETAGKSGKIADF
                     KSAVRRSLEHRMKPNDPAIINRPALIASMLDPRHKHLQFLLPAIQIAAKSKLMQLASFL
                     ESEAAPSAVAEPAEPTEPAAKKPGETEKKSAIAMLLGEDYAKKTDGNAVENELENYFRE
                     PCPSLECNPLEWWKVNAQRFPRLAKLAKSYLCIPGSSVPAERVFSTAGLTSKSVVLKTD
                     TRACQHVNFSQ"
     promoter        complement(2471..2489)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2515..2739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2785..3213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3227..3556
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3407..3542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3722..3843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3892..3908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3916..3932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3940..3970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3985..4006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4294..4882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5053..5913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5914..6018)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcgcctttgt gtcaggtagc agccgcaaat gtgataccca gcgcgcggaa
      901 aaattaaccg agctgctgtg caccatgatt accgaagata tgctgccggt gagcgttgtt
      961 gaaggcgcgg gctttcgtgc gttaatgagc tttgtggagc ctgagtacca tgtgcctggt
     1021 cgccaaaccg ttaccagcca actggaacgc tggtatgaga actgcgtgaa ctcattacgt
     1081 gagcgcttag gcaaagcgga caaagtggcg ttcaccaccg attgctggac cgcgttaaac
     1141 gcggaaagct acatgaccgt gacctgccac tatattgaaa actgggaact gcgcagcgcc
     1201 gttttacaga ccgaaagcat gcctgaacgt cacaccgcgg agaacattgc cgaaaagctg
     1261 aacgccgcgg ttgaacgttg gggcctggaa ggtcgcgttt tagcatgcgt gcacgataac
     1321 gccagcaacg tggtgttagc gaacatgcct cgctacgtgg cgtgggaaag cgttaactgc
     1381 ttcgcgcaca ccttacagct ggcgattaac gatggcttca gcggcagcgt ggatcatgtt
     1441 atcgcggcgg cgtcacgttt agttgcgcac ttccaccata gcaccgtggc ggcaaaggca
     1501 ttagaacgca agcagaccca gttacaggcc aaacagcatc atctgattca gagctgcaag
     1561 acccattgga acagcgccta tgagatgttc gagcgcttaa gcgaacagcg ctggcctata
     1621 actgctgtgc tgagcgatcg cagcgtgacc aaagttagcg acgcgcaaac cttagagctg
     1681 caggatgaac agtggcagct gatcgaggac attctgcctg tgctgagcac cctgaaatgt
     1741 gccacgactg ccatgtcagc ggaacatagc gccagcatta gcaacatcta cccgatttgc
     1801 aacagcctgc tgaacaccca tctgaaaagc gagactgcag gcaaaagcgg caaaattgcg
     1861 gatttcaaga gcgcggttcg ccgtagcctg gaacatcgca tgaagccgaa cgatccggcg
     1921 attatcaatc gcccggccct gattgcaagc atgctggacc ctcgccataa gcacttacag
     1981 ttcctgctgc ctgcgattca gattgcggcg aagagcaaat taatgcagct ggcgagcttc
     2041 ttagagagcg aagccgcacc tagcgcagtt gcagaacctg cggaacctac tgaaccggcg
     2101 gcaaaaaaac cgggcgaaac ggaaaaaaaa agcgcgatcg ccatgttact gggcgaggat
     2161 tatgcgaaga aaaccgatgg caacgccgtg gaaaacgagc tggaaaacta tttccgcgag
     2221 ccttgcccta gcctggaatg taacccgctg gagtggtgga aggttaacgc acagcgcttt
     2281 ccgcgtttag cgaaactggc caagagctat ctgtgtatcc cgggtagcag cgttcctgca
     2341 gaacgtgtgt ttagcaccgc aggcctgact agcaaaagcg tggtgctgaa aactgatacc
     2401 cgcgcctgtc agcatgtgaa cttcagccag tgatgagcgg ccgctcgagc atgcatctag
     2461 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     2521 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2581 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2641 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2701 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2761 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2821 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2881 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2941 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3001 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3061 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3121 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3181 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     3241 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3301 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3361 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3421 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3481 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3541 cctaggcttt tgcaaaaagc tccccgaaat gaccgaccaa gcgacgccca acctgccatc
     3601 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     3661 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     3721 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3841 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     3901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     6061 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6121 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     6181 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     6241 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
