LOCUS       Exported                6970 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3120
                     /codon_start=1
                     /label=hAT-17_Croc
                     /translation="MTVSSTPISLPMLSLPVPEEELELDLDLSAIAREILGKEGESSEF
                     VLHTPQVSPRARSPSPEGTSEEAEVEVAEASGSAESAPPVAVPLPAEEGEKAASAHAPA
                     PQKRAGSVVWDHFELADDPTYAICLHCRAKTSRGKGTKHMSTTGMLMHLRRHHPLALAP
                     PQPGTSGSVPKGKSPSRSKAPVPPKQRQATLEQWGKGGQKGGRVARASEITQSIGEMLA
                     VDGQPFSLVERPGFRRLMALVAPSYQVPARTTFSRTVVPSLYEACREYLREELRKAGPQ
                     VALHFTSDIWSSRGGDHAYLSLTGHWCDQSGRRWALLQAEVMDESHTATEIMGAMNRLV
                     QGWLVGQGELTRGFMVTDNGANMVKAVRDANFVGIRCVAHKFHLIVRDALEGDRAAGDG
                     ASTTSKLISKCRKVAGYFHRSIKGGKMLRDKQAELNIPQHKIMQDVETRWNSTYLMLER
                     LVEQQKAIHEMALLGEIGISGPLNRAEWDTISQILVVLKPFLEATETLSAGDALLSQVI
                     PVVRELQSQMESFQGINVPGWGKPLSPDVQALVRRLKEGIRKRLDPLRSSTVHVLAATC
                     DPRVKGSVCGSQSLNQWTEVLVKRVREAERRRRGDVEEGDPLSRASTPSTSQSPPPPQA
                     LPMWAKGMASMVGSRGTRPHHQAGSAQALVAAYLAEDVEPLLCDPLAYWASRSQMWPDL
                     ATVAREHLSCPPTSVPSERVFSIAGDVVTPHRTSLDPGLVEQLVFLKVNLPLLGFPKLQ
                     VQTE"
     promoter        complement(3158..3176)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3202..3426
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3472..3900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3914..4243
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4094..4229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4409..4530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4579..4595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4603..4619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4627..4657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4672..4693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4981..5569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5740..6600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6601..6705)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga ccgttagcag cacccctatc agcctgccga tgttaagcct gcctgtgcct
      901 gaagaggaac tggaactgga tctggacctg tcagcgattg cgcgtgaaat tctgggcaaa
      961 gaaggcgaaa gcagcgaatt tgtgctgcat acccctcaag ttagccctcg tgcccgttca
     1021 ccttcacctg aaggtaccag cgaagaggcg gaagtggaag ttgcagaagc gagcggtagc
     1081 gcagaaagcg cacctcctgt tgcagttcct ctgcctgcag aggaaggcga aaaagcagcg
     1141 tcagcacatg cacctgcacc tcaaaaacgc gcaggtagcg ttgtttggga ccattttgaa
     1201 ctggcggatg atcctaccta tgcgatctgc ctgcattgcc gtgccaaaac cagccgtggt
     1261 aaaggcacca aacatatgag caccaccggc atgttaatgc atctgcgccg ccatcatcct
     1321 ttagcgttag cgcctcctca acctggcacc agcggttcag ttcctaaggg caaaagccct
     1381 agccgcagca aagcacctgt gcctcctaaa caacgccaag ccaccttaga gcaatggggt
     1441 aaaggcggcc aaaaaggtgg tcgtgttgcg cgtgcaagcg aaatcaccca gagcattggc
     1501 gaaatgctgg ccgttgatgg ccagcctttt agcttagtgg agcgtcctgg ctttcgtcgc
     1561 ttaatggcgt tagttgcgcc gagctatcaa gttcctgccc gcaccacttt tagccgtacc
     1621 gtggttccga gcctgtatga agcgtgccgc gaatatctgc gcgaagaact gcgtaaagcg
     1681 ggtcctcagg ttgcgttaca tttcaccagc gatatttgga gcagccgcgg tggtgatcac
     1741 gcgtacttaa gcctgaccgg ccattggtgc gatcaaagcg gtcgtcgttg ggcgttatta
     1801 caagccgagg tgatggatga aagccatacc gccaccgaga taatgggtgc catgaaccgt
     1861 ttagttcagg gctggttagt gggccaaggt gaattaaccc gcggctttat ggtgaccgac
     1921 aacggcgcga acatggttaa agctgtgcgc gacgcgaatt ttgtgggcat tcgctgcgtt
     1981 gcgcacaagt tccacctgat tgtgcgcgat gcgttagaag gcgatcgtgc cgcaggtgat
     2041 ggtgcgagca ctaccagcaa gctgattagc aaatgccgca aggtggcggg ttatttccat
     2101 cgcagcatta aaggcggcaa aatgctgcgc gataaacagg ccgaactgaa cattcctcag
     2161 cataagatta tgcaggatgt ggagactcgc tggaatagca cctacctgat gttagagcgc
     2221 ctggtggaac aacagaaggc gatccacgaa atggcactgt taggcgagat tggtatcagc
     2281 ggccctttaa atcgcgcgga atgggatacc attagccaga ttctggtggt gctgaaaccg
     2341 tttctggaag cgaccgaaac cttatcagca ggcgatgcgt tattaagcca ggtgattcct
     2401 gtggtgcgcg aattacagag ccagatggaa agctttcagg gcatcaacgt gcctggttgg
     2461 ggtaaaccgc tgagccctga tgtgcaagcg ttagtgcgcc gtctgaaaga aggcattcgc
     2521 aagcgtttag accctctgcg tagcagcacc gttcacgttt tagcggcgac ctgcgatcct
     2581 cgtgttaaag gcagcgtttg tggcagccag agcctgaatc agtggaccga agtgttagtg
     2641 aagcgcgtgc gtgaagcaga acgtcgtcgt cgtggcgatg ttgaagaagg cgacccgtta
     2701 agccgtgcaa gcaccccttc aaccagccaa agccctcctc ctcctcaagc tctgcctatg
     2761 tgggcaaaag gcatggcgag catggttggt tcacgtggta cccgtcctca tcatcaagcg
     2821 ggtagcgccc aagcattagt tgcagcatac ctggcggaag atgtggagcc gctgttatgt
     2881 gatccgctgg cctattgggc aagccgtagc caaatgtggc ctgatttagc caccgtggcg
     2941 cgtgaacatc tgagctgtcc tcctaccagc gttcctagcg agcgtgtttt tagcatcgcg
     3001 ggcgatgttg tgacccctca tcgcaccagc ttagatcctg gcctggtgga acaactggtg
     3061 ttcctgaagg tgaacctgcc gttactgggc ttccctaaac tgcaggtgca gaccgaataa
     3121 tgagcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
     3181 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3241 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3301 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3361 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3421 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3781 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3841 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3901 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3961 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4021 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4081 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4141 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4201 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgaaatgac
     4261 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6721 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6781 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6841 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     6901 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     6961 tatacgcgtt
//
