LOCUS       Exported                6976 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3126
                     /codon_start=1
                     /label=hAT-4_AMi
                     /translation="MSSPSVSLPLPEEELELEPGVLSALAQAILGTEGESEFVLHTPQS
                     SPRPRSPPESSTSEEVEVEVVEASGSAEAAPPAVVPQPAEEWDKAGSPPSTQKRASSVV
                     WEHFELADDPRFAICQHCRRHISRGKDVKHFSTTAMLLHLRRQHPLALLAAQPGTSASV
                     PKGKSPARSKAPFPPKQRQATLEQWGKAADRGGRVAKASEITQTIGEMLALDSQPFSLV
                     ERPGFRRLIALLAPSYQVPARTTFSRTVVPSLYEACREYLREELRKAGPQVALHFTSDI
                     WSSRGGDHAYLSLTGHWCDQSGRRWALLQAEVLDESHTAREIMGAMNRMVQGWLVGQAE
                     LTRGFMVTDNGANMVKAVHDANFVGIRCVAHRLHLIVRDALEGDRAAGDGAATTTATSQ
                     LISKCRKVAGYFHRSIKGGKMLREKQAELSIPQHKIMQDVETRWNSTYLMLERLVEQQK
                     AIHEMALLGEIGISGPLNRAEWGTISQILVVLKPFLEATETLSAGDALLSQVIPVVREL
                     ENQMEKFQGINVPGWGRPLSPDVQALVRRLKEGIRRRLDPLRSSTVHMMAGMCDPRVKG
                     SMCTGSTKTLDHWTQVLVNKVREAEVQRRGDVEEGDPLSRASTPSTTSTGTSTSQSPPR
                     QPLPMWAMGMASMLGSKRTRPQPRAGSAEASVATYLAEDVEPLDCDPLAYWASRSQMWQ
                     DLATVARQHLSCPPTSVPSERVFSIAGDVVTPHRTCLDPALVEQLVFLKVNLPLLGFPK
                     LHLQTD"
     promoter        complement(3164..3182)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3208..3432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3478..3906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3920..4249
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4100..4235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4415..4536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4585..4601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4609..4625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4633..4663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4678..4699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4987..5575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5746..6606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6607..6711)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgt caagccctag cgtgagcctg cctttacctg aggaagagct ggaactggag
      901 cctggtgtgt taagcgcact ggcacaagcg attttaggca ccgagggcga aagcgagttt
      961 gtgctgcata cccctcaaag cagccctcgt cctcgtagcc ctcctgaaag cagcaccagc
     1021 gaagaggtgg aagtggaagt ggttgaagcg agcggttcag cagaagcagc acctcctgcg
     1081 gttgttcctc aacctgcgga agagtgggat aaagcgggta gccctccgag cacccaaaaa
     1141 cgtgcgagca gcgttgtgtg ggaacacttt gagctggcgg atgatcctcg ctttgcgatc
     1201 tgtcaacact gtcgccgcca tatcagccgt ggcaaagatg tgaaacattt tagcaccacc
     1261 gccatgttac tgcatctgcg ccgccaacat cctttagcgt tactggcagc gcaacctggt
     1321 acctcagcca gcgtgcctaa gggtaaaagc cctgcccgta gcaaagcgcc ttttcctcct
     1381 aaacagcgcc aagcgacctt agaacaatgg ggtaaggcgg cggatcgtgg tggtcgtgtt
     1441 gcaaaagcaa gcgagattac ccagaccatc ggcgaaatgc tggcgttaga tagccagccg
     1501 ttcagcttag tggaacgccc tggttttcgc cgtctgattg cattactggc gccgagctat
     1561 caagttcctg cccgcaccac cttttcacgt accgtggtgc cgagcttata tgaagcctgc
     1621 cgcgagtatc tgcgcgaaga gttacgcaaa gccggtcctc aagtggccct gcattttacc
     1681 agcgacattt ggtcaagccg tggcggtgat catgcgtatc tgagcttaac cggccactgg
     1741 tgtgatcagt caggtcgtcg ctgggcatta ttacaagcgg aagtgctgga cgaaagccat
     1801 accgcccgcg aaataatggg tgcgatgaac cgtatggtgc agggctggtt agttggtcag
     1861 gcagaactga cccgtggctt catggtgact gataacggcg ccaacatggt gaaagcggtg
     1921 cacgatgcca actttgtggg catccgctgt gttgcacatc gcctgcattt aattgtgcgc
     1981 gacgccttag aaggtgatcg tgcggcaggt gatggtgcag caaccactac cgcaaccagc
     2041 cagctgatta gcaagtgccg caaagtggcc ggctactttc atcgcagcat taaaggcggc
     2101 aaaatgctgc gtgaaaagca ggcggagctg agcattcctc agcacaagat tatgcaggat
     2161 gtggaaaccc gttggaacag cacctatctg atgctggagc gcttagtgga acagcagaaa
     2221 gcgattcacg aaatggcgct gttaggcgag attggcatca gcggccctct gaatcgtgca
     2281 gaatggggca ccattagcca gatcctggtg gtgctgaaac ctttcctgga agcgaccgaa
     2341 actctgagcg cgggtgatgc gttattaagc caggtgattc cggttgttcg cgagctggaa
     2401 aaccagatgg agaaattcca gggcattaac gtgcctggtt ggggccgtcc tttaagccct
     2461 gatgtgcaag cattagtgcg ccgcctgaaa gaaggtattc gccgccgctt agatccttta
     2521 cgtagcagca ccgttcacat gatggcgggt atgtgtgatc ctcgcgtgaa aggctcaatg
     2581 tgtaccggca gcaccaaaac cctggatcac tggacccagg tgctggtgaa caaagtgcgc
     2641 gaggcggaag ttcaacgtcg cggcgatgtt gaagaaggcg atccgttaag ccgtgcaagc
     2701 acccctagca ccactagcac tggtaccagc accagccaat cacctcctcg ccaaccttta
     2761 cctatgtggg cgatgggcat ggcgtcaatg ttaggcagca aacgcacccg tcctcaacct
     2821 cgtgcaggca gcgcagaagc aagcgttgcg acctatctgg ccgaagatgt ggaaccgctg
     2881 gattgcgacc ctttagcata ctgggcgagc cgtagccaaa tgtggcaaga tctggcaacc
     2941 gtggcccgtc aacatttaag ctgccctcct accagcgttc ctagcgaacg cgtttttagc
     3001 atcgcgggcg atgttgttac tcctcaccgc acctgtttag atcctgccct ggttgaacag
     3061 ctggtgttcc tgaaggtgaa cctgcctctg ctgggctttc ctaaactgca tctgcagacc
     3121 gattgatgag cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
     3181 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3241 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3301 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     3361 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     3421 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     3481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3901 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3961 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4021 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4081 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4141 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4201 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccga
     4261 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4321 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4381 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     4441 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4501 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     4561 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4621 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4681 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4741 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5401 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5701 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5761 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5821 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5881 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5941 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6001 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6061 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6121 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6181 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6241 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6301 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6361 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6421 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6481 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6541 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6601 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6721 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     6781 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     6841 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     6901 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     6961 gccagatata cgcgtt
//
