LOCUS       Exported                6361 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2511
                     /codon_start=1
                     /label=Tigger4
                     /translation="MSKRPADTPMGNSDKKKRKHLCLSIAQKVKLLEKLDSGVSVKRLT
                     EEYGVGMTTIYDLKKQKDKLLKFYAESDEQKLMKNRKTLHKAKNEDLDRVLKEWIRQRR
                     SEHMPLNGMLIMKQAKIYHDELKIEGNCEYSTGWLQKFKKRHGIKFLKICGDKASADHE
                     AAEKFIDEFAKVIADENLTPEQVYNADETSLFWRYCPRKTLTTADETAPTGIKDAKDRI
                     TVLGCANAAGTHKCKLAVIGKSLRPRCFQGVNFLPVHYYANKKAWITRDIFSDWFHKHF
                     VPAARAHCREAGLDDDCKILLFLDNCSAHPPAEILIKNNVYAMYFPPNVTSLIQPCDQG
                     ILRSMKSKYKNTFLNSMLAAVNRGVGVEGFQKEFSMKDAIYAVANAWNTVTKDTVVHAW
                     HNLWPATMFSDDDEQGGDFEGFRMSSEKKMMSDLLTYAKNIPSESVSKLEEVDIEEVFN
                     IDNEAPVVHSLTDGEIAEMVLNQGDRDNSDDEDDVVNTAEKVPIDDMVKMCDGLIEGLE
                     QRAFITEQEIMSVYKIKERLLRQKPLLMRQMTLEETF"
     promoter        complement(2549..2567)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2593..2817
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2863..3291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3305..3634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3485..3620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3800..3921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3970..3986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3994..4010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4018..4048)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4063..4084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4372..4960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5131..5991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5992..6096)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcaaacgtcc tgcggacacc cctatgggta acagcgacaa gaaaaaacgc
      901 aaacatctgt gcctgagcat tgcccagaaa gtgaaactgc tggaaaagct ggatagcggc
      961 gtgagcgtta aacgcctgac cgaggaatat ggcgtgggca tgaccaccat ctatgacctg
     1021 aaaaagcaga aagacaagct gctgaaattc tacgcggaaa gcgatgagca gaagctgatg
     1081 aagaaccgca aaaccctgca caaggcgaaa aacgaagacc tggaccgcgt gctgaaagag
     1141 tggattcgcc agcgtcgcag cgaacatatg ccgctgaacg gcatgctgat tatgaaacag
     1201 gcgaagatct accatgatga gctgaagatt gagggcaact gcgaatatag caccggctgg
     1261 ctgcagaaat ttaaaaaacg ccacggcatc aagtttctga agatctgcgg cgataaagcg
     1321 agcgcggatc atgaagccgc ggaaaaattt attgacgagt tcgccaaggt gattgccgat
     1381 gaaaacctga ccccggaaca ggtgtataac gccgacgaga ctagcttatt ctggcgttac
     1441 tgccctcgca aaaccttaac caccgccgat gaaaccgcgc ctaccggcat taaggatgcc
     1501 aaggatcgca ttaccgtgct gggttgtgca aatgcagcgg gtacccacaa atgtaagctg
     1561 gcggtgattg gcaaaagcct gcgccctcgt tgttttcagg gcgtgaactt cttaccggtg
     1621 cactactatg cgaacaaaaa agcctggatc acccgcgata tctttagcga ctggttccac
     1681 aaacatttcg ttcctgcggc gcgtgcacat tgtcgtgaag caggcctgga cgatgattgc
     1741 aaaatcctgc tgtttctgga caactgcagc gcacatcctc ctgcggagat tctgattaaa
     1801 aacaacgtgt acgcgatgta ttttccgccg aacgtgacca gcctgatcca gccttgtgat
     1861 cagggtatcc tgcgcagcat gaagagcaag tacaagaaca cctttctgaa cagcatgctg
     1921 gcggcggtta atcgtggcgt tggtgtggaa ggcttccaga aagaatttag catgaaggac
     1981 gcgatctatg cggttgcaaa cgcgtggaac accgtgacca aagataccgt ggtgcatgcg
     2041 tggcataact tatggccggc gaccatgttt agcgatgacg acgagcaggg cggtgatttt
     2101 gagggcttcc gcatgagcag cgaaaaaaag atgatgagcg atctgctgac ctacgcgaaa
     2161 aacattccta gcgagagcgt gagcaaactg gaagaagtgg atattgagga ggtgttcaac
     2221 attgataacg aggcgccggt tgttcattca ctgaccgatg gcgaaattgc ggaaatggtg
     2281 ctgaatcagg gcgaccgcga taacagcgat gatgaagacg acgtggtgaa caccgcggaa
     2341 aaggtgccta tcgatgatat ggtgaaaatg tgcgacggcc tgattgaagg tttagaacag
     2401 cgcgcgttca ttaccgaaca ggaaatcatg agcgtgtaca agattaaaga acgcctgctg
     2461 cgccagaaac cgttactgat gcgccagatg accctggagg aaaccttttg atgagcggcc
     2521 gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     2581 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2641 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2701 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2761 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2821 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     2881 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2941 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3001 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3061 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3121 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3181 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3301 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3361 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3541 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3601 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgaaatga ccgaccaagc
     3661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3781 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     3841 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3901 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     3961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6121 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     6181 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     6241 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     6301 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     6361 t
//
