LOCUS       Exported                6580 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2730
                     /codon_start=1
                     /label=Tigger2
                     /translation="MGPIRSATSDAGSAPKKQRKVMTLQEKVELLDMYRRLRSAAAVAR
                     HFKINESSVRTIVKKEKEIREAVAAATPAGAKTLHFLRNTFLSRIENAAFMWVQDCYKK
                     GIPIDSNMIREKAKSLYDNLKQKEGEGSKAGEFNASKGWFDNFRKRFGLKNVKITGEAA
                     SADQEAADEFPDAIKKIIEEKGYLPEQVFNADESALFWRKKMPQRTFISKEEKRAPGFK
                     AGRDRLTLLFCANAVGFMIRTALIYKAANPRALKGKDKHQLPVFWLYNKKAWTTRTLFL
                     DWFHRCFVPEVRKYLASKGLPFKVLLILDNAPGHPEPHEFNTEGVEVVYLPPNTTSLIQ
                     PLDQGVIRTFKAHYTRYSMERIVNAMEENPDRENIMKVWKDYTIEDAIVVIEKAVKAIK
                     PETINSCWRKLCPDVVHDFTGFTTEPIKEIMKEIVDMAKKVGGEGFQDMDLGEIQELID
                     TTPEELTEDDLMEMSASEPVPDDEEEDVEEAVPENKLTLDNLAEGFRLFKTAFDFFYDM
                     DPSMIRALKLKQTVEEGLVPYRNIFREMKKQKSQTEITMYFHKVTPSVPASPASPSTSS
                     TSSASATLRQQDQPLLFLLLLSLLNVKTMRMKTFMMIHFHLMNSKYIFSSL"
     promoter        complement(2768..2786)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2812..3036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3082..3510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3524..3853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3704..3839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4019..4140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4189..4205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4213..4229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4237..4267)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4282..4303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4591..5179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5350..6210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6211..6315)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg gccctattcg tagcgcgacc tcagatgcag gtagcgcccc taaaaaacag
      901 cgcaaggtga tgaccctgca ggaaaaagtg gaactgctgg acatgtatcg tcgtttacgt
      961 agcgcagcag cagttgcacg ccactttaaa attaacgaaa gcagcgtgcg caccatcgtg
     1021 aaaaaggaga aggaaattcg cgaagccgtt gcagcagcaa ctcctgcggg tgcgaaaacc
     1081 ttacatttcc tgcgcaacac cttcctgagc cgcatagaaa acgccgcgtt catgtgggtg
     1141 caggattgct acaaaaaagg cattccgatc gacagcaaca tgattcgcga gaaggccaag
     1201 agcctgtacg ataacctgaa acagaaagaa ggcgaaggca gcaaagcggg cgagttcaac
     1261 gcaagcaaag gctggtttga caactttcgc aaacgcttcg gcctgaagaa cgtgaaaatt
     1321 accggcgaag cggcatcagc agaccaagaa gcggcagacg aatttccgga tgcgattaag
     1381 aagatcatcg aggagaaagg ctatctgcct gaacaggtgt tcaacgcgga cgaaagcgcc
     1441 ttattttggc gcaagaagat gcctcagcgc accttcatta gcaaagaaga gaagcgcgcg
     1501 cctggtttta aagcgggtcg tgatcgtctg accctgctgt tttgtgcgaa cgcggtgggt
     1561 ttcatgattc gcaccgcgct gatttataaa gccgccaacc ctcgtgcgtt aaagggcaaa
     1621 gacaagcatc agctgccggt gttctggctg tataacaaga aagcgtggac cacccgcacc
     1681 ttatttctgg actggttcca tcgctgcttt gtgcctgaag tgcgcaaata tctggcgagc
     1741 aagggcctgc cgtttaaagt gctgctgatc ctggataatg cgcctggtca tccggaaccg
     1801 catgaattta acaccgaagg cgtggaggtt gtgtatctgc cgcctaacac cacctcactg
     1861 attcagccgc tggatcaggg tgtgatccgc acctttaaag cccattatac ccgctatagc
     1921 atggagcgca ttgtgaacgc gatggaagaa aacccggacc gcgagaacat tatgaaggtg
     1981 tggaaggact ataccatcga agatgcgatc gtggtgatcg aaaaagccgt gaaggccatc
     2041 aaaccggaaa ccattaacag ctgctggcgc aaactgtgtc ctgacgtggt gcatgatttt
     2101 accggcttca ccaccgagcc tattaaggaa atcatgaagg agattgtgga catggcgaaa
     2161 aaagttggcg gcgagggctt tcaagatatg gacctgggcg agattcagga gctgatcgac
     2221 accacccctg aggaactgac cgaagacgat ctgatggaga tgagcgcgag cgaacctgtg
     2281 cctgatgacg aagaggagga cgtggaagaa gccgtgccgg aaaacaaact gaccctggac
     2341 aacttagcgg agggctttcg cttattcaaa accgccttcg acttcttcta tgacatggac
     2401 cctagcatga tccgcgcgct gaaactgaaa cagaccgtgg aagaaggctt agtgccgtat
     2461 cgcaacattt ttcgcgagat gaagaaacag aagagccaga ccgagattac catgtacttc
     2521 cataaagtga ccccgagcgt tcctgcaagc cctgcatcac cttcaaccag cagcactagc
     2581 agcgcgtcag caaccttacg tcaacaggac cagcctctgc tgttcctgtt actgctgagc
     2641 ctgctgaacg tgaaaaccat gcgcatgaaa accttcatga tgatccactt ccatctgatg
     2701 aacagcaagt atatcttcag cagcctgtaa tgagcggccg ctcgagcatg catctagagg
     2761 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     2821 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2881 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2941 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3001 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3061 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3121 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3181 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3241 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3301 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3361 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3421 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3481 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     3541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3601 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3781 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3841 aggcttttgc aaaaagctcc ccgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3901 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3961 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     4021 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4081 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4141 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4201 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4261 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4321 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4381 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     6361 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6421 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     6481 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     6541 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
