LOCUS       Exported                6445 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2595
                     /codon_start=1
                     /label=hAT-19_Crp
                     /translation="MNAKKSSKTAKYVSALDRARQYPAGTLHADGGRLFCTSCNVTLDV
                     SRKTSIDRHLESEAHMKRKAAAEAEGQSKKQATVSSLFKRTTESSLARREAAFSLVEAF
                     AAANIPLEKLDHPKLRDYLNQNVPNAGSFPRANKLRQDYLPAVIASHVQSTKAALAEYH
                     SFSIVADEATDPQDNYVLHILFVPGSWTDRDLPVFQTEPVHLTSVNFSTVSQAIVKVIV
                     SYGVDFNKISAFLSDNAMYMCKAFSDVLQGMLPNAVHVTCNAHILSLVSDIWRTQFPEV
                     DSLVSSFKKAFKHCPGRKVRYRESVASQSGDSHGTVPLPPEPVLTRWNSWFNAVRYHAK
                     HMQHYQQFVSEELEITPSTQCMLKLRDLLGRGDIQDQVTFVADNATRFMELLTWFEGRQ
                     AVIHHAHNKLMDLISWVEDRASQELSSCPRENQRRRDLFQAIAVKLNQYYRYDPSLPPR
                     FRQPAAPFLSAVRILDPNQVPLLSCDPQLLKAIPGWGKEHDNERAAYVNFVKECQMPVP
                     VPVFWDSVCDRFPHLYALARRCLTIPTNSVDAERAISVYGQVFTPQRQRMSPATAAGCS
                     MLAFNT"
     promoter        complement(2633..2651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2677..2901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2947..3375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3389..3718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3569..3704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3884..4005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4054..4070)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4078..4094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4102..4132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4147..4168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4456..5044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5215..6075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6076..6180)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga acgcgaagaa gagcagcaag accgccaagt atgtgagcgc gctggatcgt
      901 gcacgccaat atcctgcagg caccctgcat gcagacggtg gtcgcttatt ttgtaccagc
      961 tgcaacgtga ccttagatgt gagccgcaag accagcattg atcgccatct ggagagcgaa
     1021 gcgcatatga agcgcaaagc ggcagcggaa gcagaaggcc agagcaagaa acaagccacc
     1081 gtgagcagcc tgtttaaacg caccaccgaa agctcattag cgcgtcgtga agcggcattt
     1141 agcctggtgg aagcatttgc ggcggcgaat atccctctgg aaaaactgga tcatccgaaa
     1201 ctgcgcgact atctgaacca gaacgtgcct aacgcaggct catttcctcg cgcgaacaaa
     1261 ctgcgccagg actatttacc tgccgtgatc gcgtcacatg tgcagagcac caaagcagcg
     1321 ctggcggaat atcatagctt tagcattgtg gccgatgagg cgaccgatcc tcaggacaac
     1381 tacgtgctgc atatcctgtt tgtgcctggc agctggactg atcgtgatct gcctgtgttt
     1441 cagaccgaac cggtgcactt aaccagcgtg aacttcagca ccgtgagcca ggccattgtg
     1501 aaagtgattg tgagctacgg cgtggatttt aacaaaatca gcgcgtttct gagcgataac
     1561 gcgatgtaca tgtgcaaggc gtttagcgat gtgctgcagg gcatgttacc taacgccgtg
     1621 catgttacct gcaacgcgca tattctgagc ctggtgagcg atatttggcg cacccagttt
     1681 cctgaagtgg acagcctggt gagcagcttc aaaaaggcgt ttaaacattg ccctggccgc
     1741 aaagttcgct atcgcgaaag cgttgcgagc caaagcggtg atagccatgg taccgtgcct
     1801 ttacctcctg agcctgtgtt aacccgctgg aacagctggt ttaacgccgt gcgctaccat
     1861 gcgaaacaca tgcagcatta ccagcagttt gtgagcgagg agctggaaat taccccgagc
     1921 acccagtgca tgctgaaact gcgcgatctg ttaggtcgtg gcgacattca ggatcaggtg
     1981 accttcgtgg cagataacgc gacccgcttt atggaactgc tgacctggtt tgaaggtcgc
     2041 caggccgtta ttcatcatgc gcacaacaaa ctgatggatc tgattagctg ggtggaagat
     2101 cgcgcgagcc aagaactgag cagctgccct cgtgaaaatc agcgtcgccg cgatctgttt
     2161 caagcgatcg cggtgaagct gaaccagtac tatcgctacg accctagctt acctccgcgt
     2221 tttcgtcaac ctgccgcgcc ttttttatca gccgtgcgca tcttagaccc gaaccaggtt
     2281 cctctgctga gctgtgatcc gcagctgctg aaagcaattc cgggctgggg taaagagcac
     2341 gacaatgaac gcgccgcgta tgtgaacttt gtgaaggaat gccagatgcc tgtgccggtg
     2401 cctgtgtttt gggatagcgt gtgcgatcgc ttccctcacc tgtatgcctt agcacgccgc
     2461 tgtttaacca taccgaccaa tagcgtggat gcggaacgcg cgattagcgt ttacggccag
     2521 gtgtttaccc ctcagcgtca gcgtatgtca cctgcgactg cggcaggttg tagcatgctg
     2581 gcgtttaaca cctaatgagc ggccgctcga gcatgcatct agagggccct attctatagt
     2641 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     2701 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2761 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2821 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2881 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     2941 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3001 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3061 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3121 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3181 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3241 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3301 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3361 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3421 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3541 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3601 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     3661 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3721 gctccccgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     3781 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     3841 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     3901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     4021 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4861 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6181 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     6241 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     6301 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     6361 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     6421 gatgtacggg ccagatatac gcgtt
//
