LOCUS       Exported                6613 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2763
                     /codon_start=1
                     /label=Tigger5
                     /translation="MLGSGSEPQLPVSHAITRVNNRYSTVYCVASVFWILCFVFSHPIM
                     STKCPSVSPASGEKKRKAITLEMKLKIIAQHEGGKPVMAIARELGLSQSTISTILKDKK
                     RISDAVKSSASVKSTVITKKRAGPIDDMEKLLVTWMEDQIQKFIPLSLLTIQAKARSLF
                     DTLKERADDPTYTQMFIASHGWFQRFKRRHNFHNVKVSGEAASADTEGAKAFKEELHRI
                     IVDEKYLPEQIFNVNETSLFWKRMPEHTYIHQESKTMPGFKAFKDRVMLLLGGNVAGFK
                     LKPFLIYHSENPRAFKNVSKHTLPIYYHHNKKAWMTSALFEDWFLNCFIPQAREYCRQN
                     NIPFKILLILDNAPGHPQHIGDMHPDVKVVYLPPNTTALIQPMDQGAIAAFKAYYLRQT
                     FAQAVEATESGRTLREFWKGFNILNAIRNIAAAWEEVTQQCMNGIWKKVLKTYVNTFKG
                     FNKDSAVDEIVSNKILVLGKQLELDIDEEDIHELVGIEAEELSNEELIELEEERSKEVE
                     AEEEEVIPEAPRKFTAKKLAEAFATISSGVRKLEEMDVNYERFARADRQIQDALACYRE
                     IYNEKKKQTVQSKLDIFLKNTMPAKPSTSVDAPVPSTSYSQASSEEREIDDPVAVASPS
                     SSN"
     promoter        complement(2801..2819)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2845..3069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3115..3543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3557..3886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3737..3872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4052..4173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4222..4238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4246..4262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4270..4300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4315..4336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4624..5212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5383..6243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6244..6348)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgt taggtagcgg tagcgaacct caactgccgg tgagccatgc aattacccgc
      901 gtgaacaacc gctatagcac cgtgtattgc gtggccagcg tgttttggat cctgtgcttt
      961 gtgtttagcc atcctatcat gagcaccaaa tgcccgagcg tttcacctgc aagcggcgaa
     1021 aaaaaacgca aagcgattac cctggaaatg aagctgaaga tcatcgccca acacgaaggc
     1081 ggtaagccgg ttatggcgat tgcacgcgaa ttaggcctga gccaaagcac catcagcacc
     1141 attctgaaag ataaaaagcg catcagcgat gccgttaaga gcagcgcgag cgtgaaaagc
     1201 accgtgatta ccaaaaaacg cgccggcccg attgatgata tggaaaaact gctggtgacc
     1261 tggatggaag accagatcca gaagttcatt cctctgagcc tgctgaccat ccaagcgaaa
     1321 gcacgcagcc tgtttgatac cctgaaggaa cgcgcagacg atcctaccta cacccagatg
     1381 tttattgcga gccacggctg gtttcagcgt tttaagcgcc gccataactt tcataacgtg
     1441 aaggtgagcg gcgaagcagc aagcgcagat accgaaggtg cgaaggcctt caaggaagaa
     1501 ctgcatcgca ttattgtgga tgaaaagtat ctgccggagc agatttttaa cgtgaacgaa
     1561 acgagcctgt tctggaaacg catgccggaa cacacctata ttcaccagga aagcaagacc
     1621 atgccgggct tcaaagcctt caaggatcgc gtgatgctgc tgttaggcgg caatgtggcg
     1681 ggctttaagc tgaaaccttt cctgatctac cacagcgaaa acccgcgcgc gtttaaaaac
     1741 gtgagcaaac ataccctgcc gatttattac caccacaaca agaaagcctg gatgaccagc
     1801 gcactgttcg aagactggtt cctgaactgc ttcattcctc aggcgcgcga atattgccgc
     1861 cagaacaaca ttccgtttaa gatcctgctg attctggata acgcgccggg tcatcctcag
     1921 catattggcg atatgcaccc ggatgtgaaa gtggtgtatc tgcctcctaa caccaccgca
     1981 ctgatccagc ctatggacca aggtgcgatt gcggccttta aagcgtatta tctgcgccag
     2041 acctttgcgc aagcagttga agcaaccgaa agcggtcgta ccttacgcga gttctggaaa
     2101 ggctttaaca ttctgaacgc catccgcaat atcgcagcgg cgtgggaaga agtgacccaa
     2161 cagtgcatga acggcatttg gaagaaagtg ctgaagacct atgtgaacac ctttaagggc
     2221 ttcaacaagg acagcgcggt ggatgagatt gtgagcaaca aaatcctggt gctgggcaaa
     2281 cagctggaac tggatattga tgaggaagac attcacgagc tggtgggcat tgaagcggag
     2341 gaactgagca acgaagagct gattgagctg gaggaagaac gcagcaaaga agtggaagcg
     2401 gaggaggaag aagtgattcc tgaagcccct cgcaaattta ccgcgaagaa actggcggaa
     2461 gcgtttgcga ccattagcag cggtgtgcgc aaactggaag aaatggacgt gaactatgaa
     2521 cgctttgcgc gcgcggatcg tcaaatccaa gatgccttag cgtgctaccg cgagatttat
     2581 aacgagaaga agaaacagac cgtgcagagc aaactggata ttttcctgaa gaacaccatg
     2641 ccggcgaaac ctagcaccag cgttgatgca cctgtgccta gcaccagcta tagccaagcc
     2701 agcagcgagg aacgcgagat tgatgatcct gtggccgttg catcacctag cagctcaaac
     2761 tgatgagcgg ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     2821 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2881 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2941 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3001 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3061 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3421 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3541 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3661 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3721 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3781 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3841 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgaaat
     3901 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3961 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4021 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     4081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4201 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4261 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4321 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4381 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4441 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5041 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5341 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5401 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5461 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5521 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5581 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5641 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5701 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5761 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5821 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5881 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5941 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6001 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6061 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6361 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     6421 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     6481 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     6541 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     6601 agatatacgc gtt
//
