LOCUS       Exported                5314 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ZCP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 31-03-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..113
                     /label=Ftw
                     /note="Ftw"
     primer_bind     121..138
                     /label=M13F
                     /note="M13F"
     gene            182..3388
                     /label=96C-TR
                     /note="96C-TR"
     primer_bind     complement(768..779)
                     /label=T7ter
                     /note="T7ter"
     gene            1022..1616
                     /label=puromycin_transacetylase
                     /note="puromycin_transacetylase"
     gene            1680..2399
                     /label=eGFP
                     /note="eGFP"
     primer_bind     complement(3416..3433)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(3459..3475)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3601..4220)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(4375..5235)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(5277..5305)
                     /note="Amp promoter"
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac agtggtacct cggagatcga acgccccaga actcgaacaa ttcggtgatc
      241 gaacgaaatt tccgaccatt ttttgccccg gacaccgaac agaaattcgg aggtcgaacg
      301 ggcgactgga aagaacctgt ttgggccgag acgcgtaggc cagggcgacc gagacaaagg
      361 ccgaatgacc cagtttacgc gtagctctcg cccagtgcag acccgcaaaa catcgttccg
      421 ttaacttttt cgttaatttc ctttatttta gtgttttttg tgtttatccg tacacaaccg
      481 gtgctagcaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      541 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      601 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      661 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      721 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
      781 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
      841 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
      901 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
      961 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaac gccaccatgg
     1021 ggaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     1081 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     1141 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     1201 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     1261 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     1321 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     1381 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     1441 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     1501 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     1561 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccatcg
     1621 atgccactaa cttctccctg ttgaaacaag caggggatgt cgaagagaat cccgggccaa
     1681 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1741 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1801 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1861 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1921 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1981 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2041 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2101 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2161 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2221 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2281 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2341 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     2401 taattaagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2461 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2521 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     2581 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     2641 gggctctatg gcttctgagg cgcgccctcg agccacagag gaactccaac accttcacga
     2701 tgagcagcaa cagatggcaa aagaggacat gtcttcagag gaggaggagg gaagggatcc
     2761 tgcccctagt tcactgataa aggaaatgat tgcaaaatgg gctgagttac acaattttgt
     2821 agaaaaatat cacccagata aggcagtagc cagcagagca ggcgattttt ataatgataa
     2881 tgcaatgagt catttcagaa acatcttaaa acggaggcag aaacaatcct ctcttgataa
     2941 gtttttagta aaaaggccct caagtgaatc acagttagtg ctagaaaaaa ggcaaagaga
     3001 agaaagaacc ccagaagggc agttacccga cgtttttatg gagggggact ccccttccaa
     3061 gcaacactga cacctcctcc tcctcctcac caccttccac tcaagccatc cactctcctt
     3121 actacaggta aagtgaagtt caatgttcat ttattctatt aattataact gtgtttcttg
     3181 tatgctttta tgtactgtac tgtattttaa tattgtaaac tttgtgtaaa aatggcccat
     3241 aataggagca tccttgggtg gggaaccaat taatgctatt tccattcatt cttatgggga
     3301 aaattgattc ggtgttcgaa caattcggtg ttcgaacacg gttttggaac gaattaagtt
     3361 cgatctccga ggtaccactg tagggcccag agacggagtc actgccaacc gagacggtca
     3421 tagctgtttc ctgtgtgccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttacccacag aatcagggga
     3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4381 atgcttaatc agtgaggcac ctatctcagc gatctgtctc tttcgttcat ccatagttgc
     4441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4501 tgcaataata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4681 tgccatcgct acaggcatcg tggtatcacg ctcgtcgttt ggtatggctt cattcagctc
     4741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgcgcaaaa aagcggttag
     4801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gccgtgttat cactcatggt
     4861 tatggcagca ctacataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5281 tctcatgagc ggatacatat ttgaatgtat ttag
//
