LOCUS       Exported                4814 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    BJA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 04-03-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..113
                     /label=Ftw
                     /note="Ftw"
     primer_bind     121..138
                     /label=M13F
                     /note="M13F"
     gene            182..2888
                     /label=111C-TR
                     /note="111C-TR"
     primer_bind     complement(776..787)
                     /label=T7ter
                     /note="T7ter"
     gene            1030..1624
                     /label=puromycin_transacetylase
                     /note="puromycin_transacetylase"
     gene            1688..2407
                     /label=eGFP
                     /note="eGFP"
     primer_bind     complement(2916..2933)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(2959..2975)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3101..3720)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(3875..4735)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(4777..4805)
                     /note="Amp promoter"
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac aaccccaaat cagaaaaagt tgggacagta tggaaaacgc aaataaaaaa
      241 gaaaatagtg atttccaaat ttactttgac ttgtatttca ttgcagacaa tatgaacaca
      301 aaatatttca tgttttgttt gtggtcaact tcatttcatt tgtaaatata catcctttcc
      361 tgtcattcag acctgcaaca cattccaaaa aatgggacag gagcaattta gggctagtaa
      421 tcaggtaaat tggttaaata atgatgtgat ttgaaacagg tgatgtcaac aggtgattgt
      481 aattaccggt gctagcaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
      541 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
      601 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      661 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      721 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      781 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      841 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      901 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
      961 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaacgc
     1021 caccatgggg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     1081 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     1141 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     1201 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     1261 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     1321 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1381 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1441 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1501 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1561 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1621 tgccatcgat gccactaact tctccctgtt gaaacaagca ggggatgtcg aagagaatcc
     1681 cgggccaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1741 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1801 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1861 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1921 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1981 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2041 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2101 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2161 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2221 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2281 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2341 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2401 caagtaatta attaagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2461 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2521 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2581 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2641 gatgcggtgg gctctatggc ttctgaggcg cgccctcgag atgctttact gttccaactt
     2701 ttttaaaatg tgttgcaaga accaaaattg aaatacgtgt ttattttaaa aaaaaataat
     2761 catgaggaac acattaaata atgtttgttg tattgtctgc aatgaaatac aagtcaaagt
     2821 acattaactt tttttatttg cgttttccat actgtcccaa ctttttctga tttggggttg
     2881 tagggcccag agacggagtc actgccaacc gagacggtca tagctgtttc ctgtgtgccg
     2941 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3001 actcaaaggc ggtaatacgg ttacccacag aatcagggga taacgcagga aagaacatgt
     3061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3541 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3781 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3901 ctatctcagc gatctgtctc tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3961 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaataata ccgcgggacc
     4021 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4081 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4141 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg
     4201 tggtatcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4261 gagttacatg atcccccatg ttgcgcaaaa aagcggttag ctccttcggt cctccgatcg
     4321 ttgtcagaag taagttggcc gccgtgttat cactcatggt tatggcagca ctacataatt
     4381 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4441 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4501 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4561 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4621 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4681 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4741 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4801 ttgaatgtat ttag
//
