LOCUS       Exported                4767 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    QER
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 31-03-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..113
                     /label=Ftw
                     /note="Ftw"
     primer_bind     121..138
                     /label=M13F
                     /note="M13F"
     gene            182..2841
                     /label=118C-TR
                     /note="118C-TR"
     primer_bind     complement(679..690)
                     /label=T7ter
                     /note="T7ter"
     gene            933..1527
                     /label=puromycin_transacetylase
                     /note="puromycin_transacetylase"
     gene            1591..2310
                     /label=eGFP
                     /note="eGFP"
     primer_bind     complement(2869..2886)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(2912..2928)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3054..3673)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(3828..4688)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(4730..4758)
                     /note="Amp promoter"
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tctgcagcta tatagtaggt taaagatgga gcggtcgaaa agaagccatg tgtggaatca
      241 tttcacaaaa atcaaagaag atgctgttag atgtaagatg tgcaatacag agcttaggta
      301 cctgaacagc actggagcaa tgctgaatca tcttaagctg aagcatccct ctgtttcaag
      361 cgaaagacaa gatcagaaac aaacaacacc ggtgctagca attctaccgg gtaggggagg
      421 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
      481 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
      541 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
      601 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
      661 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
      721 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
      781 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
      841 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
      901 gcctttcgac ctgcagccaa cgccaccatg gggaccgagt acaagcccac ggtgcgcctc
      961 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     1021 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     1081 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     1141 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     1201 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     1261 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     1321 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     1381 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     1441 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     1501 tggtgcatga cccgcaagcc cggtgccatc gatgccacta acttctccct gttgaaacaa
     1561 gcaggggatg tcgaagagaa tcccgggcca atggtgagca agggcgagga gctgttcacc
     1621 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1681 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1741 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1801 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1861 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1921 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1981 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2041 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2101 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2161 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2221 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2281 actctcggca tggacgagct gtacaagtaa ttaattaagc tcgctgatca gcctcgactg
     2341 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     2401 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     2461 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2521 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcgcgccctc
     2581 gagaaatcat caatagagtt gtgatgcagc tttttgtttt tgttgttgtt gcttgtttaa
     2641 cctcaacaga accttgagat gtgaggttgt tttttttttt ctgattggat tttttttttc
     2701 atttttggaa gacaaaacat tttttaatga ggattttatg taaaaaaaaa aattgttgga
     2761 gtgtattgtt cttgaagttt taactggatc cttttgcact tttggaaggc aaaacaagga
     2821 tttaatacta tataggggcc cagagacgga gtcactgcca accgagacgg tcatagctgt
     2881 ttcctgtgtg ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2941 gcggtatcag ctcactcaaa ggcggtaata cggttaccca cagaatcagg ggataacgca
     3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3841 atcagtgagg cacctatctc agcgatctgt ctctttcgtt catccatagt tgcctgactc
     3901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaata
     3961 ataccgcggg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc
     4141 gctacaggca tcgtggtatc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4201 caacgatcaa ggcgagttac atgatccccc atgttgcgca aaaaagcggt tagctccttc
     4261 ggtcctccga tcgttgtcag aagtaagttg gccgccgtgt tatcactcat ggttatggca
     4321 gcactacata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4741 agcggataca tatttgaatg tatttag
//
