LOCUS       Exported                4955 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    IVU
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 08-04-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..113
                     /label=Ftw
                     /note="Ftw"
     primer_bind     121..138
                     /label=M13F
                     /note="M13F"
     gene            182..3029
                     /label=199C-TR
                     /note="199C-TR"
     primer_bind     complement(808..819)
                     /label=T7ter
                     /note="T7ter"
     gene            1062..1656
                     /label=puromycin_transacetylase
                     /note="puromycin_transacetylase"
     gene            1720..2439
                     /label=eGFP
                     /note="eGFP"
     primer_bind     complement(3057..3074)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(3100..3116)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3242..3861)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(4016..4876)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(4918..4946)
                     /note="Amp promoter"
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtat ttatacagac agttctcgct ttacgtaaat tcgcattacg caaattcgac
      241 tttacgtaaa gaattctgaa agaaatccgc attccaaaaa aaattacgaa aattccttac
      301 gcaaaccaga gctgtacaaa gacgctgtat gatgcgggca gcgtaaatgg tggtgggaag
      361 ctgagaagca tctctaagtc agttcccgcg tgtctgcaga cagtgagtaa tactgtgcct
      421 ctccctgtgt tcaatagcga tccttatatt ttcaccgtgg ctggcggcaa acgcgctctc
      481 ggggggaaga gcgaaccatc tcgtaagcgt aagccaaccg gtgctagcaa ttctaccggg
      541 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      601 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      661 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      721 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      781 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      841 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      901 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
      961 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     1021 catctccggg cctttcgacc tgcagccaac gccaccatgg ggaccgagta caagcccacg
     1081 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     1141 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     1201 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     1261 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     1321 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     1381 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     1441 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     1501 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     1561 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     1621 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccatcg atgccactaa cttctccctg
     1681 ttgaaacaag caggggatgt cgaagagaat cccgggccaa tggtgagcaa gggcgaggag
     1741 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1801 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1861 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1921 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1981 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2041 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2101 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2161 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2221 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2281 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2341 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2401 gccgggatca ctctcggcat ggacgagctg tacaagtaat taattaagct cgctgatcag
     2461 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2521 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2581 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2641 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     2701 cgcgccctcg aggtgcagtg ggtggggaag ggacaacata ctgcgcgcct acgttaactt
     2761 tactgtacag tactgtgcat actttatcat taactttacc tttcatttca taattctgtt
     2821 tattatcatg gtacagtatt taatatgtct gcattttagt acattttatg acttgcgtaa
     2881 aggtattatt ttgtgtatgc ctttgttaca taggctaagt gaagtgggtg ggtaggggca
     2941 aattcgcatt acgtaaaaat tactttatat agaggtctgc ggaatggtcc acttacgtaa
     3001 agcgagaact gtctgtattt atagggccca gagacggagt cactgccaac cgagacggtc
     3061 atagctgttt cctgtgtgcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttacccaca gaatcagggg
     3181 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4021 aatgcttaat cagtgaggca cctatctcag cgatctgtct ctttcgttca tccatagttg
     4081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4141 ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4321 ttgccatcgc tacaggcatc gtggtatcac gctcgtcgtt tggtatggct tcattcagct
     4381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgcgcaaa aaagcggtta
     4441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta tcactcatgg
     4501 ttatggcagc actacataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4621 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4861 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4921 gtctcatgag cggatacata tttgaatgta tttag
//
