LOCUS       Exported                4897 bp ds-DNA     circular SYN 23-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    XVW
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 31-03-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..113
                     /label=Ftw
                     /note="Ftw"
     primer_bind     121..138
                     /label=M13F
                     /note="M13F"
     gene            182..2971
                     /label=4-3007C-TR
                     /note="4-3007C-TR"
     primer_bind     complement(661..672)
                     /label=T7ter
                     /note="T7ter"
     gene            915..1509
                     /label=puromycin_transacetylase
                     /note="puromycin_transacetylase"
     gene            1573..2292
                     /label=eGFP
                     /note="eGFP"
     primer_bind     complement(2999..3016)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(3042..3058)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3184..3803)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(3958..4818)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(4860..4888)
                     /note="Amp promoter"
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac aaacccaatt ccagaaaagt tgggacgctg tacaaattgc gagtaaaata
      241 aggaatggaa tgatttgtca aatctcataa acttatattt tattcacaat agaacataga
      301 taacatatca aatgttgaaa gtgagacatt ttgaaatgtc atgccaaata ttggctcatt
      361 ttggatttca ccggtgctag caattctacc gggtagggga ggcgcttttc ccaaggcagt
      421 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
      481 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
      541 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
      601 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
      661 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
      721 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
      781 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
      841 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
      901 aacgccacca tggggaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      961 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1021 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1081 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1141 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1201 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     1261 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1321 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     1381 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1441 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1501 cccggtgcca tcgatgccac taacttctcc ctgttgaaac aagcagggga tgtcgaagag
     1561 aatcccgggc caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1621 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1681 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1741 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1801 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1861 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1921 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1981 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2041 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2101 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2161 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2221 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2281 ctgtacaagt aattaattaa gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2341 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2401 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2461 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2521 ctggggatgc ggtgggctct atggcttctg aggcgcgccc tcgagcgcag tcatgaaaag
     2581 gagaatcaga caacgaagat caaagactgc tgagcaacta gaagcctgta ttagacgaga
     2641 atgggacaac attcctattc ctaaacttga gcaacttgtc tcctcagtcc ccagacgttt
     2701 gcagactgtt ataaaaagaa gaggggatgc cacacagtgg taaacatggc cttgtcccaa
     2761 cttttttgag atgtgttgat gccatgaaat ttaaaatcaa cttatttttc ccttaaaatg
     2821 atacgttttc tcagtttaaa catttgatat gtcatctatg ttgtattctg aataaaatat
     2881 tgaaatttga aacttccaca tcattgcatt ctgtttttat tcgcaattcg tacagtgtcc
     2941 caactttttt ggaatcgggt ttgtagggcc cagagacgga gtcactgcca accgagacgg
     3001 tcatagctgt ttcctgtgtg ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttaccca cagaatcagg
     3121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctctttcgtt catccatagt
     4021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4081 tgctgcaata ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
     4141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4261 tgttgccatc gctacaggca tcgtggtatc acgctcgtcg tttggtatgg cttcattcag
     4321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgcgca aaaaagcggt
     4381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgccgtgt tatcactcat
     4441 ggttatggca gcactacata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4861 ttgtctcatg agcggataca tatttgaatg tatttag
//
