LOCUS       pLM433_CyanoTag_        8400 bp    DNA     circular UNA 21-FEB-2024
DEFINITION  Plasmid used for creating CyanoTag vectors by Golden Gate cloning
            (BspQI version).
ACCESSION   urn.local...1s-he0uia4
VERSION     urn.local...1s-he0uia4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..708
                     /note="Geneious type: Reporter"
                     /standard_name="mNeonGreen"
     misc_feature    709..717
                     /standard_name="short flexible region"
     misc_feature    718..786
                     /note="Geneious type: Reporter"
                     /standard_name="3*flag"
     terminator      787..904
                     /standard_name="TrrnB_CyanoGate"
     CDS             1054..1869
                     /standard_name="nptI"
     CDS             complement(2882..4165)
                     /standard_name="codA"
     misc_feature    <4466..5170
                     /note="Geneious type: Reporter"
                     /standard_name="mNeonGreen"
     misc_feature    5171..5179
                     /standard_name="short flexible region"
     misc_feature    5180..5248
                     /note="Geneious type: Reporter"
                     /standard_name="3*flag"
     promoter        5257..5578
                     /standard_name="ccdB 5' region"
     CDS             5579..5884
                     /standard_name="ccdB CDS"
     terminator      5885..5930
                     /standard_name="ccdB 3'UTR and terminator"
     rep_origin      5949..6537
     CDS             complement(6708..7568)
                     /standard_name="amp"
     promoter        complement(7610..7638)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     promoter        7692..8013
                     /standard_name="ccdB 5' region"
     CDS             8014..8319
                     /standard_name="ccdB CDS"
     terminator      8320..8365
                     /standard_name="ccdB 3'UTR and terminator"
     misc_feature    8374..8400
                     /standard_name="long flexible region"
     misc_feature    8400^8401
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCC
                     ATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
                     GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAA
                     GCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTC
                     AGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAG
                     GCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGC
                     CGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGAC
                     TTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACA
                     ACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCG
                     CCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCC
                     ATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCC
                     TGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGC
                     CGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAATACGACTCACTATAGGGC
                     GAATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGCGGCC"
ORIGIN      
        1 atggttagta agggtgagga agacaatatg gcaagcctgc cggcaacgca cgaactgcat
       61 atctttggtt cgatcaacgg cgtggacttt gacatggtcg gccaggggac tggtaacccg
      121 aacgatggct acgaggaact gaacctgaaa tcgacaaaag gcgatctgca attctcgcca
      181 tggattctcg tgccgcatat tgggtacggc tttcaccagt atttgccgta tccagatggc
      241 atgagtccgt ttcaagccgc tatggtcgat ggtagcggct atcaggtgca tcgcactatg
      301 cagtttgaag acggcgccag tttgacagtc aattaccgtt acacttatga aggctcgcat
      361 attaaaggtg aggcgcaagt taagggtacc gggtttcccg ccgacggtcc cgtgatgact
      421 aatagcctga ccgcggctga ttggtgccgt agcaaaaaaa cctacccgaa cgacaaaacc
      481 atcatttcca cgtttaaatg gagctatact acaggtaacg ggaagcgcta tcgctcgacg
      541 gcgcgcacta catacacgtt cgcgaaaccg atggccgcga attacctcaa aaaccagccg
      601 atgtatgtgt ttcgtaaaac cgagctgaaa catagcaaga cagaactgaa ctttaaagag
      661 tggcagaaag cattcacaga cgtcatgggc atggatgaac tgtacaaggg cggtggcgat
      721 tacaaagatc acgatggcga ttacaaagat cacgacatcg actataagga tgacgatgat
      781 aagtaggctt caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      841 tttttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      901 tgcgggaatt cgattgatcc gtcgacctgc aggggggggg gggaaagcca cgttgtgtct
      961 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1021 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     1081 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1141 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     1201 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1261 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1321 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     1381 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1441 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1501 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1561 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     1621 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1681 aaattaatag gttgtattga tgttggacgc gtcggaatcg cagaccgata ccaggatctt
     1741 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1801 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1861 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1921 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     1981 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     2041 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     2101 cgattcaggc ctggtatgag ccagcaacac cttcttcacg aggcagacct cagcgctcct
     2161 ccaccgctgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
     2221 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
     2281 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
     2341 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
     2401 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
     2461 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
     2521 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
     2581 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
     2641 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
     2701 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga atcattacca
     2761 aaaggttagg aatacggtta gccatttgcc tgcttttata tagttcatat gggattcacc
     2821 tttatgttga taagaaataa aagaaaatgc caataggata tcggcatttt cttttgcgtt
     2881 ttcaacgttt gtaatcgatg gcttctggct gctccagata tacggtggtt tgtgccggtt
     2941 gtgtgctggc aatcaccttg ccgccacgta ccgaataacg taccggaacc tgacggcgca
     3001 gcgcatcaaa cccattttca gccggcagga taatcaggtt ggcgctgttt ccggcggcaa
     3061 tgccgtaatc ctgcaaattc aacgtccttg cgctgtggtg ggtgattaaa ttcaggccat
     3121 cgttaatctg cccgtagccc atcaactggc aaacatgcag ccccatatgc agcacttgca
     3181 gcatattcgc cgttcccagc ggataccacg gatcgaagac atcatcgtga ccaaagcaga
     3241 cgttaatgcc cgattccagc atctctttaa cgcgcgtgat gccgcgacgt tttggatacg
     3301 tatcgaaacg tccttgcaga tgaatattga ccagcgggtt ggcgacaaag ttaataccgg
     3361 acattttcag caagcggaac aggcgtgagg tatacgcccc gttataggag tgcattgccg
     3421 tggtgtggct ggcggtgacg cgcgcgccca tgccttcatg gtgcgccagg gcagcaacgg
     3481 tttcgacaaa gcgcgactgc tcgtcatcga tctcatcaca gtgaacgtcg atgagacggt
     3541 cgtatttttg cgccagggcg aaggttttat gcagcgattc cacgccgtat tcacgggtaa
     3601 attcaaaatg cggaatcgcc cccactacat ctgcccctaa gcgtaacgcc tcttccagca
     3661 acgcttcacc gttgggatac gacaaaatcc cttcctgagg gaaggcgacg atttgcagat
     3721 caatccacgg cgcgacttcc tgcttcactt ccagcattgc tttcagcgca gttagcgttg
     3781 catccgaaac atcgacatgg gtacgcacat gctgaatgcc gttggcaatc tgccatttca
     3841 gcgtttgcca tgcgcgttgt ttcacatcgt catgggttaa taacgctttg cgctcggccc
     3901 agcgttcaat gccttcaaac agcgtgccgg actgattcca gttcggttgt ccggcggttt
     3961 gcgtggtgtc caggtgaata tgtggctcca caaacggcgg tataactaaa ccttgttcgg
     4021 catccaggct gttttcagtt atgggcatca cgccggattg cgcatcaatg gcgctgattt
     4081 ttccgtcctg cagatgaatc tgccacagcc cctcttcgcc tggtaaccgg gcgttaataa
     4141 ttgtttgtaa agcgttattc gacatcgttc atgtctcctt ttttatgtac tgtgttagcg
     4201 gtctgcttct tccagccctc ctgtttgaag atggcaagtt agttacgcac aataaaaaaa
     4261 gacctaaaat atgtaagggg tgacgccaaa gtatacactt tgccctttac acattttagg
     4321 tcttgcctgc tttatcagta acaaacccgc gcgatttact tttcgacctc attctattag
     4381 attctcgttt ggattgcaac tggtctattt tcctcttttg tttgatagaa aatcataaaa
     4441 ggatttgcag actacgggcc taaaggttag taagggtgag gaagacaata tggcaagcct
     4501 gccggcaacg cacgaactgc atatctttgg ttcgatcaac ggcgtggact ttgacatggt
     4561 cggccagggg actggtaacc cgaacgatgg ctacgaggaa ctgaacctga aatcgacaaa
     4621 aggcgatctg caattctcgc catggattct cgtgccgcat attgggtacg gctttcacca
     4681 gtatttgccg tatccagatg gcatgagtcc gtttcaagcc gctatggtcg atggtagcgg
     4741 ctatcaggtg catcgcacta tgcagtttga agacggcgcc agtttgacag tcaattaccg
     4801 ttacacttat gaaggctcgc atattaaagg tgaggcgcaa gttaagggta ccgggtttcc
     4861 cgccgacggt cccgtgatga ctaatagcct gaccgcggct gattggtgcc gtagcaaaaa
     4921 aacctacccg aacgacaaaa ccatcatttc cacgtttaaa tggagctata ctacaggtaa
     4981 cgggaagcgc tatcgctcga cggcgcgcac tacatacacg ttcgcgaaac cgatggccgc
     5041 gaattacctc aaaaaccagc cgatgtatgt gtttcgtaaa accgagctga aacatagcaa
     5101 gacagaactg aactttaaag agtggcagaa agcattcaca gacgtcatgg gcatggatga
     5161 actgtacaag ggcggtggcg attacaaaga tcacgatggc gattacaaag atcacgacat
     5221 cgactataag gatgacgatg ataagtagtg aagagctaaa agccagataa cagtatgcgt
     5281 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     5341 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     5401 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     5461 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     5521 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     5581 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     5641 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     5701 gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     5761 gcgcatgatg accaccgata tggccagtgt gccggtgtcc gttatcgggg aagaagtggc
     5821 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     5881 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gctcttcact
     5941 tgagactctt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6121 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6241 tccggtaact atcgtcttga gcccaacccg gtaagacacg acttatcgcc actggcagca
     6301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6361 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctggctc
     6781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6841 ataccgcgtg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7021 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7321 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcggagtcag
     7681 acttgaagag ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     7741 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     7801 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     7861 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     7921 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     7981 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     8041 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     8101 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa
     8161 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     8221 agtgtgccgg tttccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     8281 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     8341 agccagtctg caggtcgacc atagtgctct tcaggcgatc tgggtggcag cggcggccgc
//
