LOCUS       pCH68       11798 bp DNA     circular SYN 13-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Chris Hsiung
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        147..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..805
                     /label=chicken beta-actin promoter
     intron          806..1822
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1746..1768
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1827..1903
                     /label=oCH359
     primer_bind     1830..1849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1888..6537
                     /codon_start=1
                     /label=Feature 27
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLANLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRNKRPAATKKAGQAKKKKGSGGRTLVTFKDVFVDFTRE
                     EWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPGSGGRTLVTFKDV
                     FVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEPGSGGRT
                     LVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEE
                     PGSYPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             1888..5808
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLANLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     primer_bind     4836..4895
                     /label=oCH335
     primer_bind     complement(4846..4905)
                     /label=oCH334
     misc_feature    4864..4866
                     /label=E993A
     regulatory      5528..5537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5809..5856
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5869..6054
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             6067..6252
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             6265..6450
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             6457..6537
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(6509..6568)
                     /label=oCH366
     primer_bind     6509..6555
                     /label=oCH368
     primer_bind     complement(6509..6537)
                     /label=oCH360
     misc_feature    6536..6537
                     /label=KRAB domain
     CDS             6536..6537
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation=""
     misc_feature    6547..6603
                     /label=P2A
     CDS             6547..6603
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6604..7311
                     /label=enhanced monomeric blue fluorescent protein (Su
                     /label=enhanced monomeric blue fluorescent protein 
                     (Subachet al., 2011)
     primer_bind     complement(7285..7357)
                     /label=oCH358
     primer_bind     complement(7298..7344)
                     /label=oCH367
     primer_bind     7299..7358
                     /label=oCH365
     primer_bind     complement(7312..7372)
                     /label=oCH360
     CDS             7312..7314
                     /codon_start=1
                     /label=Feature 27
                     /translation=""
     misc_feature    7351..7939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7404..7424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7822..7833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7978..7995)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7984..8208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8562..8596
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8683..8702)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8684..8702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8709..8726)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8709..8725)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8718..8740)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8867..9322)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9004..9025)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9216..9235
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9349..9453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9454..9522,9523..10314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9672..9691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10485..11073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10974..10993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11227..11244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11361..11382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11397..11402,11403..11420,11421..11427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11435..11451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11440..11462
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11459..11475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11459..11475
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11494..11514
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11496..11514
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   11578..11640
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aagtaacaaa acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa
       61 aaacagtcat gttgtattat aaaataagta attagcttaa cctatacata atagaaacaa
      121 attatactta ttaaccggtc atggtcgaca ttgattattg actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      541 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      601 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccrgg
      661 gggggggggg gggggggggg gggggggggg ggsgrggcgg agaggtgcgg cggcagccaa
      721 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
      781 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
      841 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
      901 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
      961 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1021 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     1081 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     1141 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     1201 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     1261 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     1321 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     1381 gcaggtgggg gtgccgggcg gggcggggcc gcckcgggcc ggggrgggct cgggggrggg
     1441 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     1501 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     1561 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     1621 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     1681 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     1741 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     1801 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     1861 ttttggcaaa gaattgctag cgccaccatg acacagttcg agggctttac caacctgtat
     1921 caggtgagca agacactgcg gtttgagctg atcccacagg gcaagaccct gaagcacatc
     1981 caggagcagg gcttcatcga ggaggacaag gcccgcaatg atcactacaa ggagctgaag
     2041 cccatcatcg atcggatcta caagacctat gccgaccagt gcctgcagct ggtgcagctg
     2101 gattgggaga acctgagcgc cgccatcgac tcctatagaa aggagaaaac cgaggagaca
     2161 aggaacgccc tgatcgagga gcaggccaca tatcgcaatg ccatccacga ctacttcatc
     2221 ggccggacag acaacctgac cgatgccatc aataagagac acgccgagat ctacaagggc
     2281 ctgttcaagg ccgagctgtt taatggcaag gtgctgaagc agctgggcac cgtgaccaca
     2341 accgagcacg agaacgccct gctgcggagc ttcgacaagt ttacaaccta cttctccggc
     2401 ttttatgaga acaggaagaa cgtgttcagc gccgaggata tcagcacagc catcccacac
     2461 cgcatcgtgc aggacaactt ccccaagttt aaggagaatt gtcacatctt cacacgcctg
     2521 atcaccgccg tgcccagcct gcgggagcac tttgagaacg tgaagaaggc catcggcatc
     2581 ttcgtgagca cctccatcga ggaggtgttt tccttccctt tttataacca gctgctgaca
     2641 cagacccaga tcgacctgta taaccagctg ctgggaggaa tctctcggga ggcaggcacc
     2701 gagaagatca agggcctgaa cgaggtgctg aatctggcca tccagaagaa tgatgagaca
     2761 gcccacatca tcgcctccct gccacacaga ttcatccccc tgtttaagca gatcctgtcc
     2821 gataggaaca ccctgtcttt catcctggag gagtttaaga gcgacgagga agtgatccag
     2881 tccttctgca agtacaagac actgctgaga aacgagaacg tgctggagac agccgaggcc
     2941 ctgtttaacg agctgaacag catcgacctg acacacatct tcatcagcca caagaagctg
     3001 gagacaatca gcagcgccct gtgcgaccac tgggatacac tgaggaatgc cctgtatgag
     3061 cggagaatct ccgagctgac aggcaagatc accaagtctg ccaaggagaa ggtgcagcgc
     3121 agcctgaagc acgaggatat caacctgcag gagatcatct ctgccgcagg caaggagctg
     3181 agcgaggcct tcaagcagaa aaccagcgag atcctgtccc acgcacacgc cgccctggat
     3241 cagccactgc ctacaaccct gaagaagcag gaggagaagg agatcctgaa gtctcagctg
     3301 gacagcctgc tgggcctgta ccacctgctg gactggtttg ccgtggatga gtccaacgag
     3361 gtggaccccg agttctctgc ccggctgacc ggcatcaagc tggagatgga gccttctctg
     3421 agcttctaca acaaggccag aaattatgcc accaagaagc cctactccgt ggagaagttc
     3481 aagctgaact ttcagatgcc tacactggcc tctggctggg acgtgaataa ggagaagaac
     3541 aatggcgcca tcctgtttgt gaagaacggc ctgtactatc tgggcatcat gccaaagcag
     3601 aagggcaggt ataaggccct gagcttcgag cccacagaga aaaccagcga gggctttgat
     3661 aagatgtact atgactactt ccctgatgcc gccaagatga tcccaaagtg cagcacccag
     3721 ctgaaggccg tgacagccca ctttcagacc cacacaaccc ccatcctgct gtccaacaat
     3781 ttcatcgagc ctctggagat cacaaaggag atctacgacc tgaacaatcc tgagaaggag
     3841 ccaaagaagt ttcagacagc ctacgccaag aaaaccggcg accagaaggg ctacagagag
     3901 gccctgtgca agtggatcga cttcacaagg gattttctgt ccaagtatac caagacaacc
     3961 tctatcgatc tgtctagcct gcggccatcc tctcagtata aggacctggg cgagtactat
     4021 gccgagctga atcccctgct gtaccacatc agcttccaga gaatcgccga gaaggagatc
     4081 atggatgccg tggagacagg caagctgtac ctgttccaga tctataacaa ggactttgcc
     4141 aagggccacc acggcaagcc taatctgcac acactgtatt ggaccggcct gttttctcca
     4201 gagaacctgg ccaagacaag catcaagctg aatggccagg ccgagctgtt ctaccgccct
     4261 aagtccagga tgaagaggat ggcacaccgg ctgggagaga agatgctgaa caagaagctg
     4321 aaggatcaga aaaccccaat ccccgacacc ctgtaccagg agctgtacga ctatgtgaat
     4381 cacagactgt cccacgacct gtctgatgag gccagggccc tgctgcccaa cgtgatcacc
     4441 aaggaggtgt ctcacgagat catcaaggat aggcgcttta ccagcgacaa gttctttttc
     4501 cacgtgccta tcacactgaa ctatcaggcc gccaattccc catctaagtt caaccagagg
     4561 gtgaatgcct acctgaagga gcaccccgag acacctatca tcggcatcga tcggggcgag
     4621 agaaacctga tctatatcac agtgatcgac tccaccggca agatcctgga gcagcggagc
     4681 ctgaacacca tccagcagtt tgattaccag aagaagctgg acaacaggga gaaggagagg
     4741 gtggcagcaa ggcaggcctg gtctgtggtg ggcacaatca aggatctgaa gcagggctat
     4801 ctgagccagg tcatccacga gatcgtggac ctgatgatcc actaccaggc cgtggtggtg
     4861 ctggccaacc tgaatttcgg ctttaagagc aagaggaccg gcatcgccga gaaggccgtg
     4921 taccagcagt tcgagaagat gctgatcgat aagctgaatt gcctggtgct gaaggactat
     4981 ccagcagaga aagtgggagg cgtgctgaac ccataccagc tgacagacca gttcacctcc
     5041 tttgccaaga tgggcaccca gtctggcttc ctgttttacg tgcctgcccc atatacatct
     5101 aagatcgatc ccctgaccgg cttcgtggac cccttcgtgt ggaaaaccat caagaatcac
     5161 gagagccgca agcacttcct ggagggcttc gactttctgc actacgacgt gaaaaccggc
     5221 gacttcatcc tgcactttaa gatgaacaga aatctgtcct tccagagggg cctgcccggc
     5281 tttatgcctg catgggatat cgtgttcgag aagaacgaga cacagtttga cgccaagggc
     5341 acccctttca tcgccggcaa gagaatcgtg ccagtgatcg agaatcacag attcaccggc
     5401 agataccggg acctgtatcc tgccaacgag ctgatcgccc tgctggagga gaagggcatc
     5461 gtgttcaggg atggctccaa catcctgcca aagctgctgg agaatgacga ttctcacgcc
     5521 atcgacacca tggtggccct gatccgcagc gtgctgcaga tgcggaactc caatgccgcc
     5581 acaggcgagg actatatcaa cagccccgtg cgcgatctga atggcgtgtg cttcgactcc
     5641 cggtttcaga acccagagtg gcccatggac gccgatgcca atggcgccta ccacatcgcc
     5701 ctgaagggcc agctgctgct gaatcacctg aaggagagca aggatctgaa gctgcagaac
     5761 ggcatctcca atcaggactg gctggcctac atccaggagc tgcgcaacaa aaggccggcg
     5821 gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat ccggtgggcg gacccttgtt
     5881 actttcaagg atgtattcgt ggatttcaca cgcgaggagt ggaaattgtt ggatactgcc
     5941 cagcagattg tataccgcaa tgtaatgctc gaaaactaca aaaatctcgt gagtctcgga
     6001 tatcaactga ccaagcctga cgttatcctc cgattggaga aaggcgagga acctgggagc
     6061 ggaggaagga cgcttgtcac gtttaaggat gtttttgttg acttcactcg agaggagtgg
     6121 aagctgttgg atactgccca acaaattgtg tatcgcaacg tgatgctcga gaactacaag
     6181 aaccttgtgt ctttgggcta tcagctgact aaacctgatg taatactccg cctcgagaaa
     6241 ggagaggagc ctggtagtgg gggacggacc ctggtaacat tcaaggatgt attcgtggat
     6301 ttcacgcggg aagaatggaa actcctcgat actgcccaac agatagtgta tcgaaacgta
     6361 atgctcgaaa actacaaaaa cttggtcagc ctcggatacc aacttacaaa acctgatgtt
     6421 atcctcagac ttgagaaggg cgaggaaccc ggatcctacc catacgatgt tccagattac
     6481 gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga ctatgccagc
     6541 ggccgcgcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     6601 cctgtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
     6661 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     6721 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     6781 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     6841 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     6901 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     6961 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     7021 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     7081 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     7141 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     7201 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     7261 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaattaatt
     7321 aatgatcata cagtgctctg agtacgctat aatcaacctc tggattacaa aatttgtgaa
     7381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7741 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7921 ctttgggccg cctccccgca ggatccacta gagcggccgc ctagagctcg ctatcagcct
     7981 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8041 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     8101 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     8161 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     8221 aaagaaccag ctggggctcg actagagctt gcggaaccct taagcttttt tattatatat
     8281 tagtcacgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     8341 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     8401 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     8461 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     8521 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     8581 gactatcttt ctagggttaa aaaagatttg cgctttactc gacctaaact ttaaacacgt
     8641 catagaatct tcgtttgaca aaaaccacat tgtgttgggt cgccctatag tgagtcgtat
     8701 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8761 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8821 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt
     8881 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     8941 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     9121 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     9301 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9361 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9421 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9481 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9541 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9601 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9661 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9721 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9781 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9841 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9901 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9961 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    10021 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10081 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10141 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10201 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10261 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10321 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    11341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    11401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    11461 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacct
    11521 cccccggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
    11581 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
    11641 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
    11701 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
    11761 ttattaaaaa aaaacaaaaa ctcaaaattt cttctata
//
