LOCUS       pNS35       11363 bp DNA     circular SYN 26-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Nicholas Sambold
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        147..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..805
                     /label=chicken beta-actin promoter
     intron          806..1822
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1746..1768
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1823..1841
                     /label=oNS69
     primer_bind     1827..1903
                     /label=oCH357
     primer_bind     1830..1849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(1856..1890)
                     /label=oNS44
     primer_bind     complement(1857..1890)
                     /label=oNS43
     primer_bind     complement(1865..1890)
                     /label=oNS22.2
     primer_bind     complement(1865..1890)
                     /label=oNS27
     primer_bind     1891..1925
                     /label=oNS21.2
     CDS             1891..1917
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKLD"
     CDS             1927..1953
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKLD"
     CDS             1963..1989
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKLD"
     primer_bind     complement(1975..1998)
                     /label=oNS166
     primer_bind     1982..1998
                     /label=oNS175
     CDS             1999..2025
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKLD"
     CDS             2035..2060
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKL"
     CDS             2071..2096
                     /codon_start=1
                     /label=c-Myc NLS
                     /note="c-Myc NLS"
                     /translation="PAAKRVKL"
     misc_feature    2104..2107
                     /label=P2A
                     /note="P2A"
     CDS             2107..6024
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMA
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     misc_feature    2623..2625
                     /label=E174R
     misc_feature    3727..3729
                     /label=S542R
     misc_feature    3745..3747
                     /label=K548R
     primer_bind     4296..4317
                     /label=oCH178
     primer_bind     4791..4812
                     /label=oCH179
     primer_bind     4801..4838
                     /label=oCH257
     primer_bind     complement(4811..4858)
                     /label=oCH258
     misc_feature    4825..4827
                     /label=D908
     primer_bind     5286..5303
                     /label=oCH188
     primer_bind     5760..5808
                     /label=oCH260
     primer_bind     complement(5762..5816)
                     /label=oCH259
     misc_feature    5779..5781
                     /label=R1226A
     primer_bind     6024..6049
                     /label=oNS175
     primer_bind     complement(6024..6042)
                     /label=oNS166
     CDS             6025
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation=""
     CDS             6037..6063
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6068..6129)
                     /label=oNS45
     primer_bind     complement(6068..6093)
                     /label=oNS46
     primer_bind     complement(6073..6107)
                     /label=oNS18.2
     CDS             6073..6093
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     6081..6136
                     /label=oNS117
     primer_bind     complement(6081..6122)
                     /label=oNS116
     primer_bind     6082..6127
                     /label=oNS73
     primer_bind     complement(6082..6122)
                     /label=oNS72
     misc_feature    6100..6105
                     /label=XTEN80
     primer_bind     6106..6129
                     /label=oNS46
     primer_bind     6106..6126
                     /label=oNS17
     primer_bind     6106..6126
                     /label=oNS32
     misc_feature    6112..6168
                     /label=P2A
     primer_bind     6123..6172
                     /label=oNS169
     primer_bind     complement(6123..6172)
                     /label=oNS170
     CDS             6169..6876
                     /label=enhanced monomeric blue fluorescent protein (Su
                     /label=enhanced monomeric blue fluorescent protein 
                     (Subachet al., 2011)
     primer_bind     6589..6638
                     /label=oNS167
     primer_bind     complement(6589..6638)
                     /label=oNS168
     primer_bind     complement(6850..6922)
                     /label=oCH358
     misc_feature    6885..6967
                     /label=Feature 21
     misc_feature    6916..7504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6969..6989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7387..7398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7543..7560)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7549..7773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8127..8161
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8248..8267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8249..8267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8274..8291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8274..8290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8283..8305)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8432..8887)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8569..8590)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8781..8800
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8914..9018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9019..9087,9088..9879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9237..9256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10050..10638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10166..10175)
                     /label=oNS117
     primer_bind     10539..10558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10792..10809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10926..10947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10962..10967,10968..10985,10986..10992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11000..11016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11005..11027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11024..11040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11024..11040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11059..11079
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11061..11079
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   11143..11205
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aagtaacaaa acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa
       61 aaacagtcat gttgtattat aaaataagta attagcttaa cctatacata atagaaacaa
      121 attatactta ttaaccggtc atggtcgaca ttgattattg actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      541 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      601 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccrgg
      661 gggggggggg gggggggggg gggggggggg ggsgrggcgg agaggtgcgg cggcagccaa
      721 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
      781 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
      841 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
      901 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
      961 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1021 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     1081 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     1141 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     1201 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     1261 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     1321 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     1381 gcaggtgggg gtgccgggcg gggcggggcc gcckcgggcc ggggrgggct cgggggrggg
     1441 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     1501 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     1561 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     1621 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     1681 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     1741 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     1801 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     1861 ttttggcaaa gaattgctag cgccaccatg cctgctgcta aacgtgttaa gcttgatggg
     1921 ggtagcccgg cagccaagag agtcaaactc gacggcggct ccccagcggc aaaaagggtg
     1981 aaactagacg ggggtagccc cgccgcgaag cgtgtaaagc tggatggagg atcgcctgcg
     2041 gctaagcgag tcaaattaga cggtgggtca ccggctgcaa agcgcgtcaa attggatgct
     2101 agcggcacac agttcgaggg ctttaccaac ctgtatcagg tgagcaagac actgcggttt
     2161 gagctgatcc cacagggcaa gaccctgaag cacatccagg agcagggctt catcgaggag
     2221 gacaaggccc gcaatgatca ctacaaggag ctgaagccca tcatcgatcg gatctacaag
     2281 acctatgccg accagtgcct gcagctggtg cagctggatt gggagaacct gagcgccgcc
     2341 atcgactcct atagaaagga gaaaaccgag gagacaagga acgccctgat cgaggagcag
     2401 gccacatatc gcaatgccat ccacgactac ttcatcggcc ggacagacaa cctgaccgat
     2461 gccatcaata agagacacgc cgagatctac aagggcctgt tcaaggccga gctgtttaat
     2521 ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg agcacgagaa cgccctgctg
     2581 cggagcttcg acaagtttac aacctacttc tccggctttt atagaaacag gaagaacgtg
     2641 ttcagcgccg aggatatcag cacagccatc ccacaccgca tcgtgcagga caacttcccc
     2701 aagtttaagg agaattgtca catcttcaca cgcctgatca ccgccgtgcc cagcctgcgg
     2761 gagcactttg agaacgtgaa gaaggccatc ggcatcttcg tgagcacctc catcgaggag
     2821 gtgttttcct tcccttttta taaccagctg ctgacacaga cccagatcga cctgtataac
     2881 cagctgctgg gaggaatctc tcgggaggca ggcaccgaga agatcaaggg cctgaacgag
     2941 gtgctgaatc tggccatcca gaagaatgat gagacagccc acatcatcgc ctccctgcca
     3001 cacagattca tccccctgtt taagcagatc ctgtccgata ggaacaccct gtctttcatc
     3061 ctggaggagt ttaagagcga cgaggaagtg atccagtcct tctgcaagta caagacactg
     3121 ctgagaaacg agaacgtgct ggagacagcc gaggccctgt ttaacgagct gaacagcatc
     3181 gacctgacac acatcttcat cagccacaag aagctggaga caatcagcag cgccctgtgc
     3241 gaccactggg atacactgag gaatgccctg tatgagcgga gaatctccga gctgacaggc
     3301 aagatcacca agtctgccaa ggagaaggtg cagcgcagcc tgaagcacga ggatatcaac
     3361 ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg aggccttcaa gcagaaaacc
     3421 agcgagatcc tgtcccacgc acacgccgcc ctggatcagc cactgcctac aaccctgaag
     3481 aagcaggagg agaaggagat cctgaagtct cagctggaca gcctgctggg cctgtaccac
     3541 ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg accccgagtt ctctgcccgg
     3601 ctgaccggca tcaagctgga gatggagcct tctctgagct tctacaacaa ggccagaaat
     3661 tatgccacca agaagcccta ctccgtggag aagttcaagc tgaactttca gatgcctaca
     3721 ctggccagag gctgggacgt gaatagagag aagaacaatg gcgccatcct gtttgtgaag
     3781 aacggcctgt actatctggg catcatgcca aagcagaagg gcaggtataa ggccctgagc
     3841 ttcgagccca cagagaaaac cagcgagggc tttgataaga tgtactatga ctacttccct
     3901 gatgccgcca agatgatccc aaagtgcagc acccagctga aggccgtgac agcccacttt
     3961 cagacccaca caacccccat cctgctgtcc aacaatttca tcgagcctct ggagatcaca
     4021 aaggagatct acgacctgaa caatcctgag aaggagccaa agaagtttca gacagcctac
     4081 gccaagaaaa ccggcgacca gaagggctac agagaggccc tgtgcaagtg gatcgacttc
     4141 acaagggatt ttctgtccaa gtataccaag acaacctcta tcgatctgtc tagcctgcgg
     4201 ccatcctctc agtataagga cctgggcgag tactatgccg agctgaatcc cctgctgtac
     4261 cacatcagct tccagagaat cgccgagaag gagatcatgg atgccgtgga gacaggcaag
     4321 ctgtacctgt tccagatcta taacaaggac tttgccaagg gccaccacgg caagcctaat
     4381 ctgcacacac tgtattggac cggcctgttt tctccagaga acctggccaa gacaagcatc
     4441 aagctgaatg gccaggccga gctgttctac cgccctaagt ccaggatgaa gaggatggca
     4501 caccggctgg gagagaagat gctgaacaag aagctgaagg atcagaaaac cccaatcccc
     4561 gacaccctgt accaggagct gtacgactat gtgaatcaca gactgtccca cgacctgtct
     4621 gatgaggcca gggccctgct gcccaacgtg atcaccaagg aggtgtctca cgagatcatc
     4681 aaggataggc gctttaccag cgacaagttc tttttccacg tgcctatcac actgaactat
     4741 caggccgcca attccccatc taagttcaac cagagggtga atgcctacct gaaggagcac
     4801 cccgagacac ctatcatcgg catcgaccgg ggcgagagaa acctgatcta tatcacagtg
     4861 atcgactcca ccggcaagat cctggagcag cggagcctga acaccatcca gcagtttgat
     4921 taccagaaga agctggacaa cagggagaag gagagggtgg cagcaaggca ggcctggtct
     4981 gtggtgggca caatcaagga tctgaagcag ggctatctga gccaggtcat ccacgagatc
     5041 gtggacctga tgatccacta ccaggccgtg gtggtgctgg agaacctgaa tttcggcttt
     5101 aagagcaaga ggaccggcat cgccgagaag gccgtgtacc agcagttcga gaagatgctg
     5161 atcgataagc tgaattgcct ggtgctgaag gactatccag cagagaaagt gggaggcgtg
     5221 ctgaacccat accagctgac agaccagttc acctcctttg ccaagatggg cacccagtct
     5281 ggcttcctgt tttacgtgcc tgccccatat acatctaaga tcgatcccct gaccggcttc
     5341 gtggacccct tcgtgtggaa aaccatcaag aatcacgaga gccgcaagca cttcctggag
     5401 ggcttcgact ttctgcacta cgacgtgaaa accggcgact tcatcctgca ctttaagatg
     5461 aacagaaatc tgtccttcca gaggggcctg cccggcttta tgcctgcatg ggatatcgtg
     5521 ttcgagaaga acgagacaca gtttgacgcc aagggcaccc ctttcatcgc cggcaagaga
     5581 atcgtgccag tgatcgagaa tcacagattc accggcagat accgggacct gtatcctgcc
     5641 aacgagctga tcgccctgct ggaggagaag ggcatcgtgt tcagggatgg ctccaacatc
     5701 ctgccaaagc tgctggagaa tgacgattct cacgccatcg acacgatggt ggccctgatc
     5761 cgcagcgtgc tgcagatggc caactccaat gccgccacag gcgaggacta tatcaacagc
     5821 cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt ttcagaaccc agagtggcca
     5881 atggacgccg atgccaatgg cgcctaccac atcgccctga agggccagct gctgctgaat
     5941 cacctgaagg agagcaagga tctgaagctg cagaacggca tctccaatca ggactggctg
     6001 gcctacatcc aggagctgcg caacagcagg gctgactatc cctatgacgt gcccgattat
     6061 gccagcggct cccccaagaa gaagaggaag gtgggatccg gagggggccg cgctactaac
     6121 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgt gtctaagggc
     6181 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
     6241 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     6301 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
     6361 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
     6421 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     6481 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     6541 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
     6601 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
     6661 ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata tagatccaag
     6721 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     6781 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     6841 tgcgacctcc ctagcaaact ggggcacaag cttaattaat taattaatga tcatacagtg
     6901 ctctgagtac gctataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6961 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7021 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7081 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7141 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7201 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7261 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7321 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7381 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7441 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7501 ccgcaggatc cactagagcg gccgcctaga gctcgctatc agcctcgact gtgccttcta
     7561 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7621 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7681 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7741 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7801 gctcgactag agcttgcgga acccttaagc ttttttatta tatattagtc acgatatcta
     7861 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
     7921 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
     7981 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     8041 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     8101 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     8161 gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag aatcttcgtt
     8221 tgacaaaaac cacattgtgt tgggtcgccc tatagtgagt cgtattacaa ttcactggcc
     8281 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8341 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8401 caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa
     8461 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8521 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8581 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8641 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8701 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8761 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8821 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8881 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8941 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9001 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9061 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9121 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9181 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9241 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9301 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9361 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9421 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9481 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9541 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9601 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9661 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9721 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9781 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9841 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9901 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9961 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10021 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10081 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10141 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10201 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10261 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10321 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10381 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10441 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10501 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10561 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10621 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10681 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10741 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10801 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10861 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10921 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10981 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11041 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg
    11101 acagcaggct gaataataaa aaaattagaa actattattt aaccctagaa agataatcat
    11161 attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg
    11221 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
    11281 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac
    11341 aaaaactcaa aatttcttct ata
//
