LOCUS       Exported                5080 bp ds-DNA     circular SYN 11-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    265..270
                     /label=NcoI recognition site
     CDS             273..977
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    978..983
                     /label=BamHI restriction site
     misc_feature    984..995
                     /label=Linker
     CDS             996..1229
                     /codon_start=1
                     /label=PopTag
                     /translation="MVAEQLVGVSAASAAAASAFGSLSSALLMPKDGRTLEDVVRELLR
                     PLLKEWLDQNLPRIVETKVEEEVQRISRGRGA"
     misc_feature    1233..1238
                     /label=SalI restriction site
     terminator      1637..1664
                     /label=rrnb_T2_terminator
     promoter        1706..1734
                     /label=AmpR promoter
     gene            1776..2636
                     /label=Ampicillin resistance
     rep_origin      2791..3410
                     /label=pBR322 origin of replication
     misc_feature    3807..3829
                     /label=pGEX_3_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    3976..4107
                     /label=lacI
                     /label=lacI(1)
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             4108..5067
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
     misc_feature    4108..5067
                     /label=lacI
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg cagtgagcaa gggcgaggag gataacatgg
      301 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      361 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      421 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      481 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      541 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      601 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      661 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      721 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      781 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      841 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
      901 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      961 tggacgagct gtacaaggga tccggcagcg gcagcatggt cgcggaacaa cttgttggtg
     1021 tcagtgcggc ttccgctgcc gcagcgagcg cgttcggtag cttgagttct gctttattga
     1081 tgcctaagga tggtcgcacg ttggaggacg tggtacgtga gctgttgcgc ccgctgttaa
     1141 aggagtggct tgaccagaat ctgccccgta ttgtggagac taaagtggag gaagaggtac
     1201 aacgtatctc gcgtggtcgc ggagcttaat gagtcgacct gcaggcatgc aagcttggct
     1261 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     1321 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     1381 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     1441 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     1561 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     1621 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     1681 ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     2041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2281 aagccatacc aaacgacgag cgtgacacca cgatgcctac agcaatggca acaacgttgc
     2341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2941 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     3601 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     3661 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     3721 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3781 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3841 atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcatt
     3901 tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     3961 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
     4021 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     4081 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     4141 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     4201 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     4261 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     4321 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     4381 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     4441 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
     4501 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     4561 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     4621 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     4681 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     4741 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     4801 tacgacgata ccgaagacag ctcatgttat atcccgccgt caaccaccat caaacaggat
     4861 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     4921 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     4981 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5041 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
//
