LOCUS       Exported                2428 bp ds-DNA     circular SYN 01-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Kaiyi Jiang
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 26, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     exon            complement(1..630)
                     /label=Translation 630-1
     primer_bind     393..412
                     /label=Amp-R
     promoter        complement(631..735)
                     /label=AmpR promoter
     primer_bind     complement(788..820)
                     /label=Primer 4
     misc_feature    798..815
                     /label=gibson left
     promoter        842..1082
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1091..1247
                     /label=scaffold
     primer_bind     complement(1217..1247)
                     /label=Primer 1
     ncRNA           1268..1335
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(1346..1364)
                     /label=PD7899
     misc_feature    1348..1362
                     /label=gibson right
     primer_bind     complement(1350..1373)
                     /label=KJ608
     primer_bind     complement(1350..1373)
                     /label=Kj608
     rep_origin      complement(1439..2027)
                     /direction=LEFT
                     /label=ori
     primer_bind     complement(1443..1458)
                     /label=PG0397
     primer_bind     complement(1519..1538)
                     /label=pBR322ori-F
     CDS             complement(join(2198..2428,1..630))
                     /label=AmpR
     exon            complement(2198..2428)
                     /label=Translation 2337-2107
     primer_bind     complement(2263..2291)
                     /label=PD9796
ORIGIN
        1 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
       61 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
      421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
      601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
      661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
      721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgcta gctgtacaaa
      781 aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccaagg tcgggcagga
      841 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      901 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      961 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     1021 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     1081 acgaaacacc gccattcgca gcataacgaa agaaactgac aatcgattct ttcgggttcg
     1141 attctatccc acttgactca aagagtcaga gggctcgaat acattctctg cacaggtctt
     1201 gctaatgcaa gatctggggc aagaatgtgt tcgggtcaaa tgagttaatt gacttattta
     1261 tataaatggc cggcatggtc ccagcctcct cgctggcgcc ggctgggcaa catgcttcgg
     1321 catggcgaat gggacttttt ttaagcttgg gccgctcgag gtacctctct acatatgaca
     1381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2161 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2341 atccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2401 gcagaagtgg tcctgcaact ttatccgc
//
