LOCUS       LifeAct-MTED            5461 bp ds-DNA     circular     26-FEB-2024
DEFINITION  .
KEYWORDS    "marker:AmpR, seq primer R, AmpR"
FEATURES             Location/Qualifiers
     polyA_signal    117..251
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      complement(490..1078)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(1249..2040)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(2041..2109)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(2110..2214)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        2270..2584
                     /label="tight TRE promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    2278..2296
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2314..2332
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2349..2367
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2385..2403
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2421..2439
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2456..2474
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    2492..2510
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer          2558..2578
                     /label="EH-1"
                     /note="sequence: GCTCGTTTAGTGAACCGTCAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2590..2666
                     /label="EH-11 LifeAct Ultramer"
                     /note="sequence: AAACCGGTtatactGCGGCCGCCACCATGggcgtggcggatctgattaaGaaGttcgaaagcattagcaaagaagaa"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2598..2603
                     /label="filler (loop)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2607..2618
                     /label="Kozak Sequence"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2619..2666
                     /label="LifeAct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2619..2666
                     /label="Translation 2619-2666"
                     /translation="GVADLIKKFESISKEE"
     primer          2639..2686
                     /label="EH-9"
                     /note="sequence: GaaGttcgaaagcattagcaaagaagaaggctctggttccggcACTAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2642..2709)
                     /label="EH-14 LifeAct Reverse"
                     /note="sequence: TAACGGCCTCTCCTTTACTCACACTAGTgccggaaccagagccttcttctttgctaatgctttcgaaC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2667..2681
                     /label="linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2688..3380
                     /label="scarlet-I"
                     /ApEinfo_revcolor="#ff2a75"
                     /ApEinfo_fwdcolor="#ff2a75"
     CDS             2688..3380
                     /label="Translation 2688-3380"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK"
     primer          complement(2885..2904)
                     /label="EH-3"
                     /note="sequence: TAAATGCTCGGGAGCCATAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2927..2927
                     /label="Silent Mutation #1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3155..3155
                     /label="Silent Mutation #2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3277..3296
                     /label="EH-5"
                     /note="sequence: GCAAACTGGACATCACCTCA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3317..3317
                     /label="Silent Mutation #3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3381..3398
                     /label="linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             3381..3398
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GSGSGS"
     primer          3381..3440
                     /label="Primer 208"
                     /note="sequence: ggcagcggcagcggcagcgcgccgcgctttgaagcgtatatgatgaccggcgatctgatt"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3399..3458
                     /label="MTED"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             3399..3458
                     /label="Translation 3399-3458"
                     /translation="APRFEAYMMTGDLILNLSRT"
     primer          complement(3423..3482)
                     /label="Primer 209"
                     /note="sequence: CAACCCCGGAGCATGACGCGTtcaggtgcggctcaggttcagaatcagatcgccggtcat"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3459..3461
                     /label="Translation 3459-3461"
                     /translation="*"
     primer          complement(3462..3483)
                     /label="EH-13"
                     /note="sequence: CCAACCCCGGAGCATGACGCGT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3476..3986
                     /label="hPGK promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3576..3595)
                     /label="EH-4"
                     /note="sequence: GACGTGAAGAATGTGCGAGA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3996..4613
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4614..4619
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4620..4655
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4656..4658
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4659..4694
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4695..4697
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4698..4733
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4734..4742
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4773..4790)
                     /label="seq primer R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_site      4790..4845
                     /label="β-globin poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Primer          complement(4844..4863)
                     /label="Dent_Seq_11"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(5204..5220)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     Primer          complement(5204..5220)
                     /label="Dent_Seq_14"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    5228..5244
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(5252..5258)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(5259..5276)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(5277..5282)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    5297..5318
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        5377..111
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      5423..97
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG
       61 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTAACT
      121 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA
      181 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC
      241 ATGTCTGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
      301 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
      361 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
      421 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
      481 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
      541 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
      601 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
      661 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
      721 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
      781 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
      841 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
      901 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
      961 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1021 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1081 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1141 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1201 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     1261 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     1321 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     1381 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     1441 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     1501 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     1561 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     1621 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     1681 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     1741 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     1801 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     1861 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     1921 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     1981 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2041 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     2101 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     2161 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT
     2221 TCCCCGAAAA GTGCCACCTG GTCGTCGACT GGCCGCGAAT CGATATGTCG AGTTTACTCC
     2281 CTATCAGTGA TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGATGTC
     2341 GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG
     2401 AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTATCCCT
     2461 ATCAGTGATA GAGAACGTAT GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG
     2521 AGGTAGGCGT GTACGGTGGG AGGCCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT
     2581 CGCCTGGAGA AACCGGTtat actGCGGCCG CCACCATGgg cgtggcggat ctgattaaGa
     2641 aGttcgaaag cattagcaaa gaagaaggct ctggttccgg cACTAGTGTG AGTAAAGGAG
     2701 AGGCCGTTAT AAAAGAGTTC ATGCGGTTCA AGGTACACAT GGAGGGATCA ATGAACGGAC
     2761 ACGAgTTCGA AATCGAAGGG GAAGGAGAGG GCAGGCCCTA TGAGGGTACt CAGACCGCCA
     2821 AACTCAAAGT GACAAAGGGA GGCCCTTTGC CTTTCTCTTG GGACATCTTG TCACCACAAT
     2881 TTATGTATGG CTCCCGAGCA TTTATAAAAC ACCCAGCAGA CATACCtGAT TACTACAAGC
     2941 AGTCTTTCCC AGAAGGTTTT AAATGGGAAA GGGTCATGAA CTTTGAGGAT GGTGGTGCTG
     3001 TGACGGTGAC GCAGGATACT TCTTTGGAGG ACGGAACACT CATCTACAAG GTAAAGCTCA
     3061 GGGGCACGAA CTTCCCGCCG GACGGACCAG TCATGCAGAA AAAGACTATG GGATGGGAGG
     3121 CTTCCACCGA ACGGTTGTAT CCCGAGGACG GTGTcTTGAA GGGAGATATA AAAATGGCTC
     3181 TTCGGCTGAA AGATGGAGGC AGATACTTGG CAGATTTTAA GACCACGTAT AAGGCAAAAA
     3241 AACCAGTGCA AATGCCGGGA GCATATAACG TTGACCGCAA ACTGGACATC ACCTCACATA
     3301 ACGAGGATTA TACAGTgGTA GAACAGTACG AAAGAAGCGA AGGGAGGCAC AGCACCGGGG
     3361 GTATGGATGA GCTGTATAAG ggcagcggca gcggcagcgc gccgcgcttt gaagcgtata
     3421 tgatgaccgg cgatctgatt ctgaacctga gccgcacctg aACGCGTCAT GCTCCGGGGT
     3481 TGGGGTTGCG CCTTTTCCAA GGCAGCCCTG GGTTTGCGCA GGGACGCGGC TGCTCTGGGC
     3541 GTGGTTCCGG GAAACGCAGC GGCGCCGACC CTGGGTCTCG CACATTCTTC ACGTCCGTTC
     3601 GCAGCGTCAC CCGGATCTTC GCCGCTACCC TTGTGGGCCC CCCGGCGACG CTTCCTGCTC
     3661 CGCCCCTAAG TCGGGAAGGT TCCTTGCGGT TCGCGGCGTG CCGGACGTGA CAAACGGAAG
     3721 CCGCACGTCT CACTAGTACC CTCGCAGACG GACAGCGCCA GGGAGCAATG GCAGCGCGCC
     3781 GACCGCGATG GGCTGTGGCC AATAGCGGCT GCTCAGCAGG GCGCGCCGAG AGCAGCGGCC
     3841 GGGAAGGGGC GGTGCGGGAG GCGGGGTGTG GGGCGGTAGT GTGGGCCCTG TTCCTGCCCG
     3901 CGCGGTGTTC CGCATTCTGC AAGCCTCCGG AGCGCACGTC GGCAGTCGGC TCCCTCGTTG
     3961 ACCGAATCAC CGACCTCTCT CCCCAGCAAT TCACCATGTC TAGACTGGAC AAGAGCAAAG
     4021 TCATAAACGG AGCTCTGGAA TTACTCAATG GTGTCGGTAT CGAAGGCCTG ACGACAAGGA
     4081 AACTCGCTCA AAAGCTGGGA GTTGAGCAGC CTACCCTGTA CTGGCACGTG AAGAACAAGC
     4141 GGGCCCTGCT CGATGCCCTG CCAATCGAGA TGCTGGACAG GCATCATACC CACTTCTGCC
     4201 CCCTGGAAGG CGAGTCATGG CAAGACTTTC TGCGGAACAA CGCCAAGTCA TACCGCTGTG
     4261 CTCTCCTCTC ACATCGCGAC GGGGCTAAAG TGCATCTCGG CACCCGCCCA ACAGAGAAAC
     4321 AGTACGAAAC CCTGGAAAAT CAGCTCGCGT TCCTGTGTCA GCAAGGCTTC TCCCTGGAGA
     4381 ACGCACTGTA CGCTCTGTCC GCCGTGGGCC ACTTTACACT GGGCTGCGTA TTGGAGGAAC
     4441 AGGAGCATCA AGTAGCAAAA GAGGAAAGAG AGACACCTAC CACCGATTCT ATGCCCCCAC
     4501 TTCTGAGACA AGCAATTGAG CTGTTCGACC GGCAGGGAGC CGAACCTGCC TTCCTTTTCG
     4561 GCCTGGAACT AATCATATGT GGCCTGGAGA AACAGCTAAA GTGCGAAAGC GGCGGGCCGA
     4621 CCGACGCCCT TGACGATTTT GACTTAGACA TGCTCCCAGC CGATGCCCTT GACGACTTTG
     4681 ACCTTGATAT GCTGCCTGCT GACGCTCTTG ACGATTTTGA CCTTGACATG CTCCCCGGGT
     4741 AACTAAGTAA GGATCGATCC AAggtaccTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA
     4801 TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT
     4861 GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT
     4921 GCCATATGCT GGCTGCCATG AACAAAGGTG GCTATAAAGA GGTCATCAGT ATATGAAACA
     4981 GCCCCCTGCT GTCCATTCCT TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT
     5041 TATATTTTGT TTTGTGTTAT TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT
     5101 ACTAGCCAGA TTTTTCCTCC TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT
     5161 ATGAAGATCC CTCGACCTGC AGCCCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG
     5221 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA
     5281 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG
     5341 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC GGATCCGCAT CTCAATTAGT CAGCAACCAT
     5401 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
     5461 G
//