LOCUS       Exported                5313 bp ds-DNA     circular SYN 23-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Zipursky Lab
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2024 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    666..667
                     /label=place in frame
     misc_feature    668..728
                     /label=KDRT
     misc_feature    752..1088
                     /label=HSP70BB polyA signal
     protein_bind    complement(1095..1128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        1140..1181
                     /label=3XP3
     regulatory      1354..1363
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1360..2037
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     protein_bind    complement(2144..2177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    2192..2326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2344..2404
                     /label=KDRT
     CDS             2405..2433
                     /codon_start=1
                     /label=GS linker
                     /translation="GGGGSGGGG"
     CDS             2438..3481
                     /codon_start=1
                     /label=smGdP-10X-HA
                     /translation="YPYDVPDYAGYPYDVPDYAGYPYDVPDYAGGVSKGEELFTGVVPI
                     LVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGGGVQCFSR
                     YPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKE
                     DGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEGGYPYDVPDYAGGYPYDVP
                     DYAGGGGYPYDVPDYAGGYPYDVPDYAGGDGSVQLADHYQQNTPIGDGPVLLPDNHYLS
                     TQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGYPYDVPDYAGYPYDVPDYAGY
                     PYDVPDYA"
     CDS             2438..2464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2468..2494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2498..2524
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3053..3079
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3086..3112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3125..3151
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3158..3184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3395..3421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3425..3451
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3455..3481
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      complement(3711..4166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4193..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4298..5158
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgtggaaa cgatatcaga catttgtctg ataatgcttc attatcagac aaatgtctga
      721 tatcgtttta acgtaagcta gctagaccgg tgtcgactaa agccaaatag aaaattattc
      781 agttcctggc ttaagttttt aaaagtgata ttatttattt ggttgtaacc aaccaaaaga
      841 atgtaaataa ctaatacata attatgttag ttttaagtta gcaacaaatt gattttagct
      901 atattagcta cttggttaat aaatagaata tatttattta aagataattg cgtttttatt
      961 gtcagggagt gagtttgctt aaaaactcgt ttaggtttgt cctcccgaaa ttatttattt
     1021 aaatgcgatg gagagttggc gccgaatcga aaactttacg cgcttaaaag cacgagttgg
     1081 catccctaac gcgtataact tcgtataatg tatgctatac gaagttatcg tacgggatct
     1141 aattcaatta gagactaatt caattagagc taattcaatt aggatccaag cttatcgatt
     1201 tcgaaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     1261 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     1321 tgaacaagct aaacaatcgg ctcgaagccg gtcgccacca tggcctcctc cgaggacgtc
     1381 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     1441 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1501 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
     1561 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     1621 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1681 acccaggact cctccctcca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg
     1741 aacttcccct ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcgtccacc
     1801 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg
     1861 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg
     1921 cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac
     1981 tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagggg
     2041 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2101 aacctcccac acctccccct gaacctgaaa cataaaatga atgataactt cgtataatgt
     2161 atgctatacg aagttatcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcacta gtgatctggc
     2341 cggaaacgat atcagacatt tgtctgataa tgcttcatta tcagacaaat gtctgatatc
     2401 gtttggtggc ggcggttcgg gaggaggtgg atccatgtac ccttatgatg tgcccgatta
     2461 tgctggctac ccttatgatg ttcctgatta cgccggatat ccgtatgacg tgccagacta
     2521 cgcgggaggt gtgagcaagg gagaggagtt gtttaccggc gtggtcccca tactggtgga
     2581 gttggatggc gacgttaatg gacataaatt ctcggtccgc ggcgagggag agggagacgc
     2641 caccaatggc aagctgacgc ttaagttcat ttgcacgact ggaaaattgc ctgtcccctg
     2701 gccgaccctg gtcaccacac tgggcggagg agtgcagtgc ttctcccgtt acccagacca
     2761 catgaagcag cacgatttct tcaagagcgc aatgccggag ggatacgtgc aagaacggac
     2821 tatttccttc aaggatgacg gaacctataa gacccgtgcg gaggtcaaat tcgagggtga
     2881 caccctggtg aaccgaattg aactcaaagg aatcgatttc aaggaggatg gaaatattct
     2941 gggtcacaag ctggaataca acttcaacag ccataatgtg tacattacgg ctgataagca
     3001 gaagaacggc attaaggcca atttcaagat ccgccacaac gttgagggtg gatatcccta
     3061 cgacgtgccc gattatgcgg gcggatatcc gtatgatgtt ccagattatg ctggtggtgg
     3121 aggctatccc tatgatgtcc ccgactacgc cggaggttac ccatacgatg tgcccgacta
     3181 cgctggaggc gatggcagcg tgcagctggc agatcattat caacagaata cccccatagg
     3241 tgatggcccc gttctgcttc cagataatca ctacctttcc acccagagcg tgctttcgaa
     3301 agacccgaac gaaaagcgtg atcacatggt cctgctggag tttgtgaccg cggctggaat
     3361 caccctgggt atggacgaac tctacaaggg tggctacccc tacgatgtgc cggattacgc
     3421 tggatatccg tatgacgtac cggactatgc cggttacccg tacgatgtcc cggactacgc
     3481 tacatgttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt
     3541 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3601 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3661 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
     3721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4141 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     4201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5281 ttcgttccac tgagcgtcag accccgtaga aaa
//
