LOCUS       Exported                6744 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 27, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1341..2417
                     /codon_start=1
                     /label=HyperFAST
                     /translation="MHLANEEQNELEGAFKLGLIFTVAPYLLPKLIVSLRRTAPKMPLM
                     LEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPLYDEPFFVIVPKGHSFEELDAVS
                     PRMLGEEQVLLLTEGNCMRDQVLSIFTKVKVHMKKALSGDSYWVFVKRVGGTGGEHVVF
                     GSEDIENTMAKMDDGQLDGLAFGAIQLDGDGNILQYNAAEGDITGRDPKQVIGKNFFKD
                     VAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTLTDHSCSELAAKQRIQGLTNTLQGSS
                     IDTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAYRRNFVRPKALS
                     AMKAAIMQSQLHGVSFICD"
     CDS             2418..2441
                     /codon_start=1
                     /label=linker
                     /translation="TGVDPRGS"
     CDS             2442..2858
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="VEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQK
                     DGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWDG
                     KETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     misc_feature    2871..3459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3342..3353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3491..3967
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4007..4147
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4222..4677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4959..5063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5064..5924
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt ctgagccacc atgcacctgg ctaatgagga gcaaaacgaa ttggaaggtg
     1381 cttttaagct gggcttgatc tttaccgtcg ccccgtactt gctgcctaag ttgatcgtca
     1441 gcctgcgccg caccgccccg aagatgcctt tgatgttgga ggagaattac acccacaccc
     1501 ttaccgagtc actgaaacgc ggcgacgttg acgccatcat tgtggcagag ccgtttcaag
     1561 agccgggtat tgtgactgaa cctttgtatg acgagccgtt tttcgttatc gttccgaagg
     1621 gccatagctt cgaggagttg gatgccgtta gccctcgcat gttgggcgaa gaacaagttc
     1681 tgcttctgac cgagggcaac tgtatgcgtg accaggtgct tagcattttt accaaggtca
     1741 aggtgcacat gaagaaagcc ctttccggtg acagctattg ggtctttgtg aaacgggtag
     1801 gtggcactgg cggtgagcat gttgtctttg gcagtgagga catcgagaac actatggcca
     1861 aaatggacga cggacaactg gatgggttgg cctttggcgc aattcagctc gatggtgacg
     1921 ggaatatcct gcagtacaat gctgctgaag gagacatcac aggcagagat cccaaacagg
     1981 tgattgggaa gaacttcttc aaggatgttg cacctggaac ggattctccc gagttttacg
     2041 gcaaattcaa ggaaggcgta gcgtcaggga atctgaacac catgttcgaa tggatgatac
     2101 cgactcttac cgatcatagt tgcagcgagc ttgccgccaa acagcgcatt cagggtctca
     2161 ccaacaccct tcaaggctca agcatcgata ccatccgcca catggttgcc agtggcttgg
     2221 caatcagcgt cttgcctgcc actgccctta ccgaaaacga ccacatgctg tttagcatta
     2281 tcccgtttga aggcaccccg cctagccgcc gcgttgtcct ggcctatcgc cgcaactttg
     2341 tccgccctaa ggccctttca gccatgaaag ccgcaattat gcaaagccaa ctgcatggtg
     2401 ttagcttcat ctgcgatacc ggtgtcgacc cgcggggatc cgtcgagaaa tttgttggca
     2461 cctggaagat cgcagacagc cataattttg gtgaatacct gaaagctatc ggagccccaa
     2521 aggaattaag cgatggtggg gatgccacga cgccgacatt gtacatctcc cagaaggacg
     2581 gagacaaaat gacagtgaaa atagagaatg gacctcctac gttccttgac actcaagtaa
     2641 agttcaaatt aggggaggag ttcgacgaat ttccttctga tcgaagaaaa ggcgtaaaat
     2701 ctgtcgtgaa cttggtggga gagaagctgg tgtacgtaca aaagtgggac ggcaaggaga
     2761 cgacgtatgt ccgagagata aaggacggta aactggtcgt gacacttacg atgggagacg
     2821 tcgtggctgt gcgcagctac cggagggcga cggaatgaaa gcttatcgat aatcaacctc
     2881 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2941 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3001 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3061 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3121 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3181 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3241 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     3301 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3361 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3421 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     3481 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     3541 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     3601 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     3661 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     3721 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     3781 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     3841 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     3901 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     3961 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
     4021 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4081 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     4141 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4201 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     4441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4561 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4621 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4681 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4741 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4801 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4861 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4921 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atgt
//
