LOCUS       Exported                4535 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Dasha'S
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 27, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             166..1245
                     /label=HyPerFAST
     CDS             166..549
                     /codon_start=1
                     /label=N-OxyR
                     /translation="MHLANEEQNELEGAFKLGLIFTVAPYLLPKLIVSLRRTAPKMPLM
                     LEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPLYDEPFFVIVPKGHSFEELDAVS
                     PRMLGEEQVLLLTEGNCMRDQVLS"
     CDS             553..939
                     /codon_start=1
                     /label=cpFAST
                     /translation="FTKVKVHMKKALSGDSYWVFVKRVGGTGGEHVVFGSEDIENTMAK
                     MDDGQLDGLAFGAIQLDGDGNILQYNAAEGDITGRDPKQVIGKNFFKDVAPGTDSPEFY
                     GKFKEGVASGNLNTMFEWMIPTLTD"
     CDS             943..1245
                     /codon_start=1
                     /label=C-OxyR
                     /translation="SCSELAAKQRIQGLTNTLQGSSIDTIRHMVASGLAISVLPATALT
                     ENDHMLFSIIPFEGTPPSRRVVLAYRRNFVRPKALSAMKAAIMQSQLHGVSFICD"
     terminator      1282..1376
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1420..2079
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2144..2230
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    2385..2525
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2711..3299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3470..4330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4331..4435)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc cgggaattcg ccaccatgca cctggctaat
      181 gaggagcaaa acgaattgga aggtgctttt aagctgggct tgatctttac cgtcgccccg
      241 tacttgctgc ctaagttgat cgtcagcctg cgccgcaccg ccccgaagat gcctttgatg
      301 ttggaggaga attacaccca cacccttacc gagtcactga aacgcggcga cgttgacgcc
      361 atcattgtgg cagagccgtt tcaagagccg ggtattgtga ctgaaccttt gtatgacgag
      421 ccgtttttcg ttatcgttcc gaagggccat agcttcgagg agttggatgc cgttagccct
      481 cgcatgttgg gcgaagaaca agttctgctt ctgaccgagg gcaactgtat gcgtgaccag
      541 gtgcttagca tttttaccaa ggtcaaggtg cacatgaaga aagccctttc cggtgacagc
      601 tattgggtct ttgtgaaacg ggtaggtggc actggcggtg agcatgttgt ctttggcagt
      661 gaggacatcg agaacactat ggccaaaatg gacgacggac aactggatgg gttggccttt
      721 ggcgcaattc agctcgatgg tgacgggaat atcctgcagt acaatgctgc tgaaggagac
      781 atcacaggca gagatcccaa acaggtgatt gggaagaact tcttcaagga tgttgcacct
      841 ggaacggatt ctcccgagtt ttacggcaaa ttcaaggaag gcgtagcgtc agggaatctg
      901 aacaccatgt tcgaatggat gataccgact cttaccgatc atagttgcag cgagcttgcc
      961 gccaaacagc gcattcaggg tctcaccaac acccttcaag gctcaagcat cgataccatc
     1021 cgccacatgg ttgccagtgg cttggcaatc agcgtcttgc ctgccactgc ccttaccgaa
     1081 aacgaccaca tgctgtttag cattatcccg tttgaaggca ccccgcctag ccgccgcgtt
     1141 gtcctggcct atcgccgcaa ctttgtccgc cctaaggccc tttcagccat gaaagccgca
     1201 attatgcaaa gccaactgca tggtgttagc ttcatctgcg attgactcga ggccgactgt
     1261 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     1321 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     1381 gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     1441 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     1501 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1561 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1621 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1681 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1741 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1801 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     1861 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1921 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1981 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     2041 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     2101 tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc
     2161 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     2221 taggacaaat ccgccctcta gagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2281 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2341 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     2401 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     2461 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2521 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2581 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2641 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3121 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4441 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4501 aaaataggcg tatcacgagg ccctttcgtc ttcac
//
