LOCUS       DVK_p15A_FG             2747 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(29..46)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(163..708)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(243..262)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      822..916
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(949..1764)
                     /label="kanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1318..1318)
                     /label="G>A silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2078..2573)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(2146..2214)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     2285..2302
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(2368..2388)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(2394..2416)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(2420..2450)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      2619..2690
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      2706..2733
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 actcgagcct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
       61 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
      121 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
      181 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
      241 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
      301 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
      361 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
      481 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
      541 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
      601 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
      661 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
      721 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
      781 gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac
      841 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      901 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgcGAtt agaaaaactc
      961 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     1021 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     1081 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     1141 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     1201 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     1261 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcaag
     1321 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     1381 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     1441 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     1501 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     1561 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     1621 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     1681 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga
     1741 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     1801 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     1861 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agctcgagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     2041 tcgcggccgc ttctagagac tagtggaaga catCGCTaga gacctgcacc atatgcggtg
     2101 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     2161 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2221 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2281 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga
     2341 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggggtc tctTGCCatg
     2581 tcttctggat cggttgtcga gtaaggatct ccaggcatca aataaaacga aaggctcagt
     2641 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2701 acactggctc accttcgggt gggcctttct gcgtttatag gatccta
//