LOCUS       DVC_p15A_AE             2542 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(219..219)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(918..1413)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(986..1054)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     1125..1142
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1208..1228)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1234..1256)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1260..1290)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      1459..1530
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      1546..1573
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1616..1633)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1750..2295)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(1830..1849)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      2409..2503
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(2536..653)
                     /label="cmR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
       61 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      121 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      181 aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaacat attctcaata
      241 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      301 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      361 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      421 ttcattgcca tacgtaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      481 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      541 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      601 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      661 tccttagctc ctgaaaatct cgacaactca aaaaatacgc ccggtagtga tcttatttca
      721 ttatggtgaa agttggaacc tcttacgtgc cgatcatgcc acctgacgtc taagaaacca
      781 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      841 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
      901 gtgtggaaga catGGAGaga gacctgcacc atatgcggtg tgaaataccg cacagatgcg
      961 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     1021 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     1081 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     1141 gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg
     1201 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1261 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1321 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1381 cattaatgaa tcggccaacg cgcgggggtc tctGCTTatg tcttctggat cggttgtcga
     1441 gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1561 gggcctttct gcgtttatag gatcctaact cgagcctagg gatatattcc gcttcctcgc
     1621 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     1681 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     1741 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
     1801 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
     1861 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     1921 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1981 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
     2041 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
     2101 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
     2161 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     2221 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     2281 ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg
     2341 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgttacta
     2401 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2461 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctctcgaac
     2521 cccagagtcc cgcGAttacg cc
//