LOCUS       DVC_p15A_EF             2542 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(5..664)
                     /label="cmR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(230..230)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(929..1424)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(997..1065)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     1136..1153
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1219..1239)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1245..1267)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1271..1301)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      1470..1541
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      1557..1584
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1627..1644)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1761..2306)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(1841..1860)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      2420..2514
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcGAttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
       61 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      121 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      181 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgat acgaaaaaca
      241 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      301 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
      361 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
      421 gctcaccgtc tttcattgcc atacgtaatt ccggatgagc attcatcagg cgggcaagaa
      481 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
      541 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      601 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      661 ccattttagc ttccttagct cctgaaaatc tcgacaactc aaaaaatacg cccggtagtg
      721 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcatgc cacctgacgt
      781 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
      841 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct
      901 tctagagact agtgtggaag acatGCTTag agacctgcac catatgcggt gtgaaatacc
      961 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     1021 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     1081 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1141 aacgacggcc agtgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc
     1201 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1381 cgtgccagct gcattaatga atcggccaac gcgcgggggt ctctCGCTat gtcttctgga
     1441 tcggttgtcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1501 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1561 caccttcggg tgggcctttc tgcgtttata ggatcctaac tcgagcctag ggatatattc
     1621 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     1681 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     1741 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     1801 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1861 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     1921 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     1981 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     2041 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     2101 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     2161 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     2221 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     2281 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     2341 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     2401 agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     2461 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     2521 agctctcgaa ccccagagtc cc
//