LOCUS       DVC_p15A_FG             2542 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     27..44
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(110..130)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(136..158)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(162..192)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      361..432
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      448..475
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(518..535)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(652..1197)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(732..751)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      1311..1405
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(1438..2097)
                     /label="cmR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1663..1663)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(2362..315)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(2430..2498)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
       61 cccggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
      121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      241 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      301 cgcgcggggg tctctTGCCa tgtcttctgg atcggttgtc gagtaaggat ctccaggcat
      361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      481 aggatcctaa ctcgagccta gggatatatt ccgcttcctc gctcactgac tcgctacgct
      541 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
      601 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
      661 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
      721 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
      781 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
      841 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
      901 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
      961 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
     1021 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
     1081 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
     1141 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
     1201 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
     1261 gtagcacctg aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca
     1321 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1381 attactggat ctatcaacag gagtccaagc gagctctcga accccagagt cccgcGAtta
     1441 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1501 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1561 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1621 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     1681 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1741 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1801 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1861 gtctttcatt gccatacgta attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1921 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1981 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2041 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2101 agcttcctta gctcctgaaa atctcgacaa ctcaaaaaat acgcccggta gtgatcttat
     2161 ttcattatgg tgaaagttgg aacctcttac gtgccgatca tgccacctga cgtctaagaa
     2221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     2281 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
     2341 actagtgtgg aagacatCGC Tagagacctg caccatatgc ggtgtgaaat accgcacaga
     2401 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     2461 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     2521 gcaaggcgat taagttgggt aa
//