LOCUS       DVC_p15A_GH             2542 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(651..1146)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(719..787)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     858..875
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(941..961)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(967..989)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(993..1023)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      1192..1263
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      1279..1306
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1349..1366)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1483..2028)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(1563..1582)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      2142..2236
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(2269..386)
                     /label="cmR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2494..2494)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
       61 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      121 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgtaa ttccggatga
      181 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      241 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      301 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      361 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacaac
      421 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      481 tgccgatcat gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      541 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
      601 atttctggaa ttcgcggccg cttctagaga ctagtgtgga agacatTGCC agagacctgc
      661 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      721 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      781 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      841 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      901 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1141 gtctctACTA atgtcttctg gatcggttgt cgagtaagga tctccaggca tcaaataaaa
     1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggatccta
     1321 actcgagcct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     1381 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1441 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1501 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1561 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     1621 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1681 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1801 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     1861 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     1921 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     1981 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     2041 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     2101 gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac
     2161 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2221 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgcGAtt acgccccgcc
     2281 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2341 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2401 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2461 aaaactggtg aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc
     2521 tttagggaaa taggccaggt tt
//