LOCUS       DVK_pBR322_AE           3130 bp ds-DNA     circular     27-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     67..84
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(150..170)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(176..198)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(202..232)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     misc_feature    579..770
                     /label="ROP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(764..786)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pGEX vectors, reverse primer"
     misc_feature    787..787
                     /label="T > G to remove BsmBI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(872..1011)
                     /label="bom"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="basis of mobility region from pBR322"
     primer_bind     927..946
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     complement(1026..1043)
                     /label="L4440"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1197..1785)
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     complement(1277..1296)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pBR322 origin, forward primer"
     terminator      complement(1816..1855)
                     /label="fd terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="central terminator from bacteriophage fd (Otsuka andKunisawa, 1982)"
     misc_feature    complement(1861..2676)
                     /label="kanR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2230..2230)
                     /label="G>A silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2990..355)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(3058..3126)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
ORIGIN
        1 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
       61 cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc ctctagagtc
      121 gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      181 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      241 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      301 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcggggg tctctGCTTa
      361 tgtcttctaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
      421 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
      481 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
      541 accccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
      601 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
      661 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
      721 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
      781 tcccggcgac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
      841 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
      901 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
      961 ttgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     1021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1621 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1801 ttttctaccg gtccgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggtGA
     1861 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     1921 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     1981 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     2041 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     2101 tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca
     2161 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     2221 cgcctgagca agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     2281 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     2341 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     2401 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     2461 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     2521 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     2581 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     2641 cctggagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2701 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2761 gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga
     2821 tcagctcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2881 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2941 gatttctgga attcgcggcc gcttctagag actagtggaa gacatGGAGa gagacctgca
     3001 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     3061 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3121 acgccagctg
//