LOCUS       DVK_pBR322_GH           3130 bp ds-DNA     circular     27-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(736..1231)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(804..872)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     943..960
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1026..1046)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1052..1074)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1078..1108)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     misc_feature    1455..1646
                     /label="ROP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(1640..1662)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pGEX vectors, reverse primer"
     misc_feature    1663..1663
                     /label="T > G to remove BsmBI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1748..1887)
                     /label="bom"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="basis of mobility region from pBR322"
     primer_bind     1803..1822
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     complement(1902..1919)
                     /label="L4440"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2073..2661)
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     complement(2153..2172)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pBR322 origin, forward primer"
     terminator      complement(2692..2731)
                     /label="fd terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="central terminator from bacteriophage fd (Otsuka andKunisawa, 1982)"
     misc_feature    complement(2737..422)
                     /label="kanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(3106..3106)
                     /label="G>A silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
       61 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
      121 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
      181 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
      241 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
      301 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
      361 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
      421 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
      481 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
      541 aatcgaactt ttgctgagtt gaaggatcag ctcgagtgcc acctgacgtc taagaaacca
      601 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      661 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
      721 gtggaagaca tTGCCagaga cctgcaccat atgcggtgtg aaataccgca cagatgcgta
      781 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
      841 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      901 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      961 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
     1021 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1081 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1141 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1201 ttaatgaatc ggccaacgcg cgggggtctc tACTAatgtc ttctaacacc tacatctgta
     1261 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     1321 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     1381 atcgtgagca tcctctctcg tttcatcggt atcattaccc catgaacaga aatccccctt
     1441 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     1501 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     1561 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
     1621 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggcgacggtc acagcttgtc
     1681 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     1741 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     1801 tgcggcatca gagcagattg tactgagagt gcaccattgc ggtgtgaaat accgcacaga
     1861 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     1921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctaccggtcc gtccaaaaaa
     2701 aaaggctcca aaaggagcct ttaattgtat cggtGAttag aaaaactcat cgagcatcaa
     2761 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     2821 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     2881 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     2941 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     3001 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     3061 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcaagac gaaatacgcg
     3121 atcgctgtta
//