LOCUS       DVC_pBR322_AE           2925 bp ds-DNA     circular     27-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(636..1131)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(704..772)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     843..860
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(926..946)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(952..974)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(978..1008)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     misc_feature    1355..1546
                     /label="ROP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(1540..1562)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pGEX vectors, reverse primer"
     misc_feature    1563..1563
                     /label="T > G to remove BsmBI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1648..1787)
                     /label="bom"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="basis of mobility region from pBR322"
     primer_bind     1703..1722
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     complement(1802..1819)
                     /label="L4440"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1973..2561)
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     complement(2053..2072)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pBR322 origin, forward primer"
     terminator      complement(2592..2631)
                     /label="fd terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="central terminator from bacteriophage fd (Otsuka andKunisawa, 1982)"
     misc_feature    complement(2637..371)
                     /label="cmR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2862..2862)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
       61 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
      121 atcaccagct caccgtcttt cattgccata cgtaattccg gatgagcatt catcaggcgg
      181 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
      241 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
      301 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
      361 tttttctcca ttttagcttc cttagctcct gaaaatctcg acaactcaaa aaatacgccc
      421 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcatgccac
      481 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
      541 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
      601 ggccgcttct agagactagt gtggaagaca tGGAGagaga cctgcaccat atgcggtgtg
      661 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
      721 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      781 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      841 gttgtaaaac gacggccagt gaattcgagc tcggtacccg gggatcctct agagtcgacc
      901 tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1021 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cgggggtctc tGCTTatgtc
     1141 ttctaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     1201 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     1261 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     1321 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct
     1381 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
     1441 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
     1501 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     1561 ggcgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     1621 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     1681 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattgc
     1741 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     1801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2401 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2581 ctaccggtcc gtccaaaaaa aaaggctcca aaaggagcct ttaattgtat cggtGAttac
     2641 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     2701 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     2761 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     2821 tttaaatcaa aactggtgaa actcacccag ggattggctg atacgaaaaa catattctca
     2881 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgcc
//