LOCUS       DVC_pBR322_GH           2925 bp ds-DNA     circular     27-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(288..783)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(356..424)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     495..512
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(578..598)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(604..626)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(630..660)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     misc_feature    1007..1198
                     /label="ROP"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(1192..1214)
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pGEX vectors, reverse primer"
     misc_feature    1215..1215
                     /label="T > G to remove BsmBI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1300..1439)
                     /label="bom"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="basis of mobility region from pBR322"
     primer_bind     1355..1374
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pRS vectors, use to sequence yeast selectable marker"
     primer_bind     complement(1454..1471)
                     /label="L4440"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1625..2213)
                     /label="ori"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     complement(1705..1724)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pBR322 origin, forward primer"
     terminator      complement(2244..2283)
                     /label="fd terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="central terminator from bacteriophage fd (Otsuka andKunisawa, 1982)"
     misc_feature    complement(2289..23)
                     /label="cmR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2514..2514)
                     /label="G > T for silent mutation to remove BsmBI site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgacaactca
       61 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
      121 cgatcatgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      181 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
      241 tctggaattc gcggccgctt ctagagacta gtgtggaaga catTGCCaga gacctgcacc
      301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
      361 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      421 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      481 cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct
      541 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
      601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggggtc
      781 tctACTAatg tcttctaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
      841 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
      901 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
      961 gtatcattac cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     1021 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     1081 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     1141 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1201 catgcagctc ccggcgacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     1261 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     1321 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1381 gtgcaccatt gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1561 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1621 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1681 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1741 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1801 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1861 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1921 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1981 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2041 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2101 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2161 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2221 ctttgatctt ttctaccggt ccgtccaaaa aaaaaggctc caaaaggagc ctttaattgt
     2281 atcggtGAtt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2341 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2401 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2461 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgatacgaaa
     2521 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2581 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2641 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2701 accagctcac cgtctttcat tgccatacgt aattccggat gagcattcat caggcgggca
     2761 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2821 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2881 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggta
//