LOCUS       DVA_p15A_AH             2773 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      67..161
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(194..1054)
                     /label="ampR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1337..1842)
                     /label="lacZa part"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(1408..1476)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     1547..1564
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1630..1650)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1656..1678)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1682..1712)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      1890..1961
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      1977..2004
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2047..2064)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2181..2726)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(2261..2280)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgttactagt
       61 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      121 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc
      181 cagagtcccg cGAttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      301 taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt
      361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1201 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     1261 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
     1321 gtggaggtct caGGAGatgt cttccctgca ccatatgcgg tgtgaaatac cgcacagatg
     1381 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     1441 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1501 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1561 cagtgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     1621 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1681 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1741 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1801 tgcattaatg aatcggccaa cgcgcggggg ttaagaagac gtACTAagag accttctgga
     1861 tcggttgtcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1921 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1981 caccttcggg tgggcctttc tgcgtttata ggatcctaac tcgagcctag ggatatattc
     2041 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     2101 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     2161 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     2221 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2281 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2341 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2401 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     2461 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     2521 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     2581 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     2641 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     2701 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     2761 agatttcagt gca
//