LOCUS       DVA_p15A_AG             2773 bp ds-DNA     circular     27-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      244..338
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(371..1231)
                     /label="ampR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1514..2019)
                     /label="lacZ part"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(1585..1653)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     1724..1741
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1807..1827)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1833..1855)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1859..1889)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      2067..2138
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      2154..2181
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2224..2241)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2358..130)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(2438..2457)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
       61 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      121 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt
      181 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt tactagtgct
      241 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
      301 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctct cgaaccccag
      361 agtcccgcGA ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      481 catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat
      541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
      661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
      721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     1381 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     1441 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagactagtg
     1501 gaggtctcaG GAGatgtctt ccctgcacca tatgcggtgt gaaataccgc acagatgcgt
     1561 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     1621 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1681 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     1741 tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     1801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1981 attaatgaat cggccaacgc gcgggggtta agaagacgtT GCCagagacc ttctggatcg
     2041 gttgtcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2101 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2161 cttcgggtgg gcctttctgc gtttatagga tcctaactcg agcctaggga tatattccgc
     2221 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     2281 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     2341 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     2401 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2461 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     2521 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     2581 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     2641 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     2701 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     2761 caagttttgg tga
//