LOCUS       DVA_p15A_EH             2773 bp ds-DNA     circular     19-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(780..1285)
                     /label="lacZa part"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(851..919)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     primer_bind     990..1007
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     primer_bind     complement(1073..1093)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #7bcdc8"
     misc_binding    complement(1099..1121)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="ApEinfo_revcolor: #7ea7d8"
     promoter        complement(1125..1155)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #bc8dbf"
     terminator      1333..1404
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="transcription terminator T1 from the E. coli rrnB gene"
     terminator      1420..1447
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1490..1507)
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1624..2169)
                     /label="p15A ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
                     /direction="LEFT"
     primer_bind     complement(1704..1723)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="pBR322 origin, forward primer"
     terminator      2283..2377
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="transcription terminator from phage lambda"
     misc_feature    complement(2410..497)
                     /label="ampR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
       61 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      121 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      181 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      241 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      301 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      361 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      421 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      481 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      541 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      601 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
      661 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
      721 gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtggagg tctcaGCTTa
      781 tgtcttccct gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      841 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      901 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      961 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg
     1021 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcgta atcatggtca
     1081 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1141 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1261 caacgcgcgg gggttaagaa gacgtACTAa gagaccttct ggatcggttg tcgagtaagg
     1321 atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1381 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1441 ttctgcgttt ataggatcct aactcgagcc tagggatata ttccgcttcc tcgctcactg
     1501 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     1561 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     1621 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     1681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     1741 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     1801 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     1861 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1921 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     1981 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     2041 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     2101 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     2161 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     2221 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgtt actagtgctt
     2281 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2341 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga
     2401 gtcccgcGAt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2461 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2521 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
     2581 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2641 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2701 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2761 ggcttcattc agc
//