LOCUS       Exported                5935 bp DNA     circular SYN 20-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV_hmMHCR1_HAss
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Cedric Kiss
  TITLE     Direct Submission
  JOURNAL   Exported Feb 29, 2024 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          258..263
                     /label=source [*]
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     source          270..275
                     /label=source [5']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          276..346
                     /label=source [*]
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     promoter        1..204
                     /label=CMV promoter_promoter
                     /note="note=""CMV promoter"" - 365..568"
     primer_bind     155..175
                     /label=CMV-F
     primer_bind     264..287
                     /label=162_ADRA1Awhale_AAV_for
     primer_bind     267..290
                     /label=ADRA1a_whale_BamHI_F
     CDS             276..323
                     /codon_start=1
                     /label=secretory sequence
                     /translation="MKTIIALSYIFCLVFA"
     misc_feature    276..323
                     /label=HA secretory sequence_misc_feature
                     /note="note=""HA secretory sequence"" - 23..70"
     CDS             324..347
                     /codon_start=1
                     /label=FLAG
                     /translation="DYKDDDDA"
     CDS             324..346
                     /label=FLAG_CDS
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     primer_bind     333..365
                     /label=158_ADRA1Awhale_smBiT_for
     CDS             348..965
                     /codon_start=1
                     /label=ADRA1A
                     /translation="VFLSGNASDSSNCTHPPPPVNISKAILLGVILGGLILFGVLGNIL
                     VILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEILGYWAFGRVFCNIWAAV
                     DVLCCTASIMGLCLISIDRYWAISSPERYERKMTPKAAFIALLCVWALSLVISIGPLFG
                     WRQPAPEDETICQINEEPGYVLFSALGSFYVPLTIILVMYCRV"
     primer_bind     complement(698..728)
                     /label=53_ADRA1Awhale_ICL2G_scan_rev
     CDS             720..779
                     /codon_start=1
                     /label=RdLight1
                     /translation="WAISSPERYERKMTPKAAFI"
     primer_bind     729..758
                     /label=67_ADRA1Awhale_RdLG_ICL2G_R128K
     primer_bind     729..758
                     /label=68_ADRA1Awhale_RdLG_ICL2G_R128E
     primer_bind     729..755
                     /label=60_ADRA1Awhale_RdLG_ICL2G_P126A
     primer_bind     729..755
                     /label=61_ADRA1Awhale_RdLG_ICL2G_P126K
     primer_bind     729..755
                     /label=63_ADRA1Awhale_RdLG_ICL2G_E127A
     primer_bind     729..755
                     /label=65_ADRA1Awhale_RdLG_ICL2G_E127E
     primer_bind     729..755
                     /label=66_ADRA1Awhale_RdLG_ICL2G_R128A
     primer_bind     729..751
                     /label=64_ADRA1Awhale_RdLG_ICL2G_E127K
     primer_bind     732..755
                     /label=54_ADRA1Awhale_RdLG_ICL2G_S124A
     primer_bind     732..755
                     /label=55_ADRA1Awhale_RdLG_ICL2G_S124K
     primer_bind     732..755
                     /label=56_ADRA1Awhale_RdLG_ICL2G_S124E
     primer_bind     734..755
                     /label=59_ADRA1Awhale_RdLG_ICL2G_S125E
     primer_bind     735..755
                     /label=57_ADRA1Awhale_RdLG_ICL2G_S125A
     primer_bind     735..755
                     /label=58_ADRA1Awhale_RdLG_ICL2G_S125K
     primer_bind     738..755
                     /label=62_ADRA1Awhale_RdLG_ICL2G_P126E
     CDS             738..740
                     /codon_start=1
                     /label=Mutate to A
                     /translation="E"
     primer_bind     complement(953..977)
                     /label=203_ADRA1Awhale_TM5ins-3_rev
     primer_bind     complement(959..983)
                     /label=200_ADRA1Awhale_TM5ins-1_rev
     primer_bind     complement(959..980)
                     /label=202_ADRA1Awhale_TM5ins-2_rev
     primer_bind     complement(963..986)
                     /label=204_ADRA1Awhale_TM5ins+1_rev
     primer_bind     complement(963..986)
                     /label=205_ADRA1Awhale_TM5ins+2_rev
     primer_bind     complement(963..986)
                     /label=206_ADRA1Awhale_TM5ins+3_rev
     CDS             966..1763
                     /codon_start=1
                     /label=RdLight1
                     /translation="YRIAQKQLSSFHVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVK
                     TTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGS
                     LVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPL
                     PFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSL
                     QDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEARKDDLKRETKVL"
     primer_bind     966..987
                     /label=168_RdLG+1_for
     primer_bind     969..989
                     /label=93_For_coRdLight_RIAQKQ
     primer_bind     969..989
                     /label=7_110_For_coRdLight_RIAQKQ
     primer_bind     complement(974..993)
                     /label=215_coRdLight_rev
     primer_bind     complement(975..998)
                     /label=224_Blp1_rev
     primer_bind     complement(975..995)
                     /label=69_ADRA1Awhale_RdLGp1_F218A
     primer_bind     complement(975..995)
                     /label=70_ADRA1Awhale_RdLGp1_F218L
     primer_bind     complement(975..995)
                     /label=71_ADRA1Awhale_RdLGp1_F218S
     primer_bind     complement(975..995)
                     /label=72_ADRA1Awhale_RdLGp1_F218Q
     primer_bind     complement(975..995)
                     /label=75_ADRA1Awhale_RdLGp1_F218D
     primer_bind     complement(975..995)
                     /label=76_ADRA1Awhale_RdLGp1_F218E
     primer_bind     complement(975..995)
                     /label=77_ADRA1Awhale_RdLGp1_F218R
     primer_bind     complement(975..995)
                     /label=78_ADRA1Awhale_RdLGp1_F218K
     primer_bind     complement(975..995)
                     /label=79_ADRA1Awhale_RdLGp1_F218H
     primer_bind     complement(976..997)
                     /label=74_ADRA1Awhale_RdLGp1_F218F
     primer_bind     complement(976..996)
                     /label=73_ADRA1Awhale_RdLGp1_F218Y
     primer_bind     983..1006
                     /label=223_Blp1_for
     primer_bind     987..1007
                     /label=201_ADRA1Awhale_TM5ins-123_for
     CDS             996..1742
                     /codon_start=1
                     /label=cpmApple
                     /translation="FHVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQ
                     LPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSLVSKGEEDNM
                     AIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQ
                     FMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGVFIYKVK
                     LRGTNFPPDGPVMQKKTMGWEARKDDL"
     primer_bind     999..1023
                     /label=90_ADRA1Awhale_RdLGp1_H219H
     primer_bind     1002..1026
                     /label=80_ADRA1Awhale_RdLGp1_H219A
     primer_bind     1002..1026
                     /label=81_ADRA1Awhale_RdLGp1_H219L
     primer_bind     1002..1026
                     /label=82_ADRA1Awhale_RdLGp1_H219S
     primer_bind     1002..1026
                     /label=83_ADRA1Awhale_RdLGp1_H219Q
     primer_bind     1002..1026
                     /label=84_ADRA1Awhale_RdLGp1_H219Y
     primer_bind     1002..1026
                     /label=85_ADRA1Awhale_RdLGp1_H219F
     primer_bind     1002..1026
                     /label=86_ADRA1Awhale_RdLGp1_H219D
     primer_bind     1002..1026
                     /label=87_ADRA1Awhale_RdLGp1_H219E
     primer_bind     1002..1026
                     /label=88_ADRA1Awhale_RdLGp1_H219R
     primer_bind     1002..1026
                     /label=89_ADRA1Awhale_RdLGp1_H219K
     primer_bind     complement(1028..1052)
                     /label=92_ADRA1Awhale_RdLGp1_ICL2G_K237A
     primer_bind     1055..1074
                     /label=91_ADRA1Awhale_RdLGp1_ICL2G_K237A
     primer_bind     complement(1721..1742)
                     /label=207_ADRA1Awhale_TM6ins_rev
     primer_bind     complement(1730..1760)
                     /label=8_111_Rev_coRdLight_KRETKV
     primer_bind     complement(1736..1763)
                     /label=169_RdLG+1_rev
     primer_bind     1736..1763
                     /label=216_nLightR_for
     primer_bind     complement(1739..1768)
                     /label=226_ScaI_rev
     primer_bind     1743..1769
                     /label=211_ADRA1Awhale_TM6insp1_for
     primer_bind     1743..1769
                     /label=212_ADRA1Awhale_TM6insp2_for
     primer_bind     1743..1769
                     /label=213_ADRA1Awhale_TM6insp3_for
     primer_bind     1746..1770
                     /label=208_ADRA1Awhale_TM6insm1_for
     primer_bind     1749..1773
                     /label=209_ADRA1Awhale_TM6insm2_for
     primer_bind     1752..1779
                     /label=225_ScaI_for
     primer_bind     1752..1775
                     /label=210_ADRA1Awhale_TM6insm3_for
     CDS             1764..2228
                     /codon_start=1
                     /label=ADRA1A
                     /translation="KTLGIVVGCFVLCWLPFFLVMPIGSFFPDFRPSETVFKIAFWLGY
                     LNSCINPIIYPCSSQEFKKAFQNVLRIQCLRRKQSSKHALGYTLHPPSHALEGQYRNLV
                     RIPVGSGETFCKISKTDGVCEWKFFSSLPRGSARITVPKDQSACTTARDF"
     primer_bind     complement(1889..1922)
                     /label=118_ADRA1Awhale_44_CtermGraft_rev
     primer_bind     complement(1901..1934)
                     /label=105_ADRA1Awhale_TM7_scan_rev
     primer_bind     1935..1970
                     /label=112_ADRA1Awhale_RdLGp1_Q331A
     primer_bind     1935..1970
                     /label=113_ADRA1Awhale_RdLGp1_Q331K
     primer_bind     1935..1970
                     /label=114_ADRA1Awhale_RdLGp1_Q331E
     primer_bind     1936..1968
                     /label=106_ADRA1Awhale_RdLGp1_S329A
     primer_bind     1937..1968
                     /label=107_ADRA1Awhale_RdLGp1_S329K
     primer_bind     1937..1968
                     /label=108_ADRA1Awhale_RdLGp1_S329E
     primer_bind     1941..1973
                     /label=109_ADRA1Awhale_RdLGp1_S330A
     primer_bind     1941..1973
                     /label=110_ADRA1Awhale_RdLGp1_S330K
     primer_bind     1941..1973
                     /label=111_ADRA1Awhale_RdLGp1_S330E
     primer_bind     complement(1991..2015)
                     /label=119_ADRA1Awhale_44_CtermTrunc_rev
     primer_bind     complement(2203..2228)
                     /label=ADRA1a_whale_HindIII_R
     primer_bind     complement(2203..2226)
                     /label=121_ADRA1A_44_ERexportSeq_rev
     primer_bind     complement(2203..2225)
                     /label=159_ADRA1Awhale_smBiT_rev
     primer_bind     complement(2212..2246)
                     /label=163_ADRA1Awhale_AAV_rev
     primer_bind     2226..2246
                     /label=120_ADRA1Awhale_44_CtermTrunc_rev
     primer_bind     2229..2248
                     /label=117_ADRA1Awhale_44_CtermGraft
     primer_bind     complement(2281..2300)
                     /label=pEGFP_C2-RP
     polyA_signal    2359..2480
                     /label=SV40 poly(A) signal_polyA_signal
                     /note="note=""SV40 poly(A) signal"" - 1884..2005"
     rep_origin      complement(2487..2942)
                     /direction=LEFT
                     /label=f1 ori_rep_origin
                     /note="direction=LEFT - note=""f1 ori"" - 
                     complement(2012..2467)"
     promoter        2969..3073
                     /label=AmpR promoter_promoter
                     /note="note=""AmpR promoter"" - 2494..2598"
     promoter        3075..3432
                     /label=SV40 promoter_promoter
                     /note="note=""SV40 promoter"" - 2600..2957"
     rep_origin      3283..3418
                     /direction=RIGHT
                     /label=SV40 ori_rep_origin
                     /note="note=""SV40 ori"" - 2808..2943"
     CDS             3467..4261
                     /label=NeoR/KanR_CDS
                     /note="codon_start=1 - note=""NeoR/KanR"" - 
                     translation=""MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                      - 
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                      - 
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                      - GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMV"
     primer_bind     complement(4159..4214)
                     /label=Beta1AR_INS_FWD
     misc_feature    4493..4540
                     /label=HSV TK poly(A) signal_misc_feature
                     /note="note=""HSV TK poly(A) signal"" - 4018..4065"
     rep_origin      4869..5457
                     /direction=RIGHT
                     /label=ori_rep_origin
                     /note="direction=RIGHT - note=""ori"" - 4394..4982"
     primer_bind     4882..4891
                     /label=215_coRdLight_rev
     enhancer        5632..5935
                     /label=CMV enhancer_enhancer
                     /note="note=""CMV enhancer"" - 61..364"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttggatccg ccaccatgaa gacgatcatc gccctgagct
      301 acatcttctg cctggtgttc gccgactaca aggacgatga tgacgcagtc tttctcagtg
      361 ggaacgctag tgactcttca aattgtaccc acccaccacc tcctgttaat atcagcaagg
      421 ctattttgct gggtgtcatc ttgggaggcc tgattctgtt cggagtgctt ggtaacattc
      481 tcgtaattct ctcagtagcg tgtcaccgcc atctccacag cgttacccac tactatattg
      541 tgaacctcgc tgtagcagac ctgcttctca cgagtacggt actgccattt tccgctatct
      601 tcgaaatttt ggggtattgg gcctttggac gggttttttg taacatctgg gccgctgtgg
      661 acgtattgtg ttgtacggca tcaatcatgg gcctgtgcct tatctcaata gatcgatact
      721 gggcgattag ttcacccgag cgctacgaga gaaagatgac tccgaaggcg gcgttcattg
      781 ctctcctttg tgtctgggct ctttccttgg ttatatccat tgggcccttg ttcggctggc
      841 gccagcccgc cccggaggac gaaacaatat gtcagatcaa cgaagagcct ggatacgtcc
      901 ttttcagcgc cctcggatct ttttatgtcc cgttgacaat catcctggtc atgtattgca
      961 gagtttatcg gatcgcgcag aaacagctct ccagcttcca cgtgagcgag aggatgtatc
     1021 ctgaggacgg tgcgctgaaa tccgaaataa agaaaggcct gcgcctcaaa gacgggggcc
     1081 actacgccgc tgaggttaag acaacttaca aggctaaaaa gcctgtccag ctcccgggcg
     1141 cctacatagt cgacattaaa ctggacatag tcagccataa cgaggactac accatagtcg
     1201 aacagtgtga gcgggcggaa ggtcgccaca gtacaggagg aatggacgaa ttgtataaag
     1261 ggggcaccgg tggcagcttg gttagcaagg gagaggaaga taacatggct attataaagg
     1321 agttcatgag atttaaagtt cacatggagg gatctgtaaa cgggcatgag tttgagattg
     1381 agggagaagg agaagggcga ccgtacgagg cgttccaaac cgcgaaattg aaggtcacca
     1441 agggaggacc ccttccgttt gcttgggaca ttcttagccc acaattcatg tatggcagca
     1501 aggcgtacat aaagcacccg gcagacatac ccgactactt caaacttagc tttccagagg
     1561 gtttccgatg ggaaagagtg atgaactttg aggatggtgg catcatacac gtcaatcaag
     1621 atagcagcct tcaggacgga gtatttatat acaaagtcaa actccggggt acaaactttc
     1681 ccccggacgg gccagtcatg cagaagaaga ctatgggttg ggaggcgcgg aaagacgact
     1741 tgaagaggga gactaaagtc ctcaagacgc tcgggatagt tgttggttgt ttcgtactct
     1801 gctggttgcc ctttttcttg gtgatgccga taggtagctt ttttcccgac ttccgaccga
     1861 gtgagacagt attcaaaata gctttttggc ttggttattt gaactcctgt attaatccca
     1921 ttatatatcc ctgttcatct caagagttta aaaaggcttt tcaaaacgtg ttgcgcatac
     1981 aatgcctgcg cagaaagcag tcttctaagc acgcactcgg ttatacgctc caccccccat
     2041 cccatgctct cgagggacag tatagaaacc tcgtgcgaat acctgtgggt agtggcgaaa
     2101 cattttgcaa aatcagtaaa acagacgggg tatgtgaatg gaaatttttt tcatccttgc
     2161 cacgcggatc tgccagaata acagttccta aggaccagtc cgcttgcaca actgcccggg
     2221 acttttaaga attcagagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     2281 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2341 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2401 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2461 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2521 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2581 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2641 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2701 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2761 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2821 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2881 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2941 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3061 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3121 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3181 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3241 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3421 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3481 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3541 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3601 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     3661 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3721 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3781 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3841 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3901 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     3961 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4021 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4081 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4141 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4201 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4261 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4321 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4381 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4441 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4501 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4561 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     4621 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     4681 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     4741 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4981 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5041 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5101 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5161 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5221 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5281 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5341 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5401 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5461 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5521 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
     5581 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5641 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5701 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5761 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     5821 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5881 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
