LOCUS       1792                 6832 bp    DNA     circular SYN 21-FEB-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      4176..4604
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4948..5739
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2145..3032
                     /note="pLannotate"
                     /label="HaloTag®"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             5760..6620
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(6791..6832,1..547)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        871..901
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    835..856
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1920..2122
                     /note="pLannotate"
                     /label="mini-AID (fragment)"
                     /database="snapgene"
                     /identity="97.0"
                     /match_length="99.5"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(3994..4012)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1899..1916
                     /note="pLannotate"
                     /label="thrombin site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    909..925
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4015..4172
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             4614..4916
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="98.0"
                     /match_length="21.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    1825..1865
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="62.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             730..822
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        5743..5759
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="26.6"
                     /fragment="True"
                     /other="promoter"
     CDS             1038..1223
                     /note="pLannotate"
                     /label="SMC4 (fragment)"
                     /database="swissprot"
                     /identity="87.1"
                     /match_length="4.4"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca
     1021 gatattttct taatcatttc agtgttcgac cacctaagaa aagttggaaa aagatcttca
     1081 acctttcggg aggagagaaa acacttagtt cattggcttt agtatttgct cttcaccact
     1141 acaagcccac tcccctttac ttcatggatg agattgatgc agcccttgat tttaaaaatg
     1201 tgtccattgt tgcattttat atatatgtaa gtaatcattt tgggattttc attccagaaa
     1261 cttttagggg tatcctaatc attacacatg gaattcttta tttcttacaa ttgagctttt
     1321 tctttattgt ctaataactc catctccatc tcctctggta tcagtggaga ccaaattatt
     1381 ttttatttga accaaatatt tccattttgg aggagtaaaa tataaattaa aaatgcaaat
     1441 tagtagcaga atcacagcta gaatcctagg tcatcttcct agatcttttc cactagaagt
     1501 caaaataacc aaattccttt cacactcaaa tactgtttta tgaattattc ctgccatcat
     1561 tcctttacat gttatcaatg taatgtttat tgtccaaaaa atatttgagg ttacatgttt
     1621 gtgtgattta cctgttatta cttaatgttt gacttttctt tgtttctctt taggaacaaa
     1681 caaaaaatgc acagttcata ataatttctc ttcgaaataa tatgtttgag atttcggata
     1741 gacttattgg aatttacaag acatacaaca taacaaaaag tgttgctgta aatccgaagg
     1801 agatagcgtc aaaaggactt tgttactcag atctcgagct caagcttcga attctgcagt
     1861 cgacgagcag tcaccatcat caccatcata gcagcggcct ggtgccgcgc ggcagccata
     1921 aggagaagag tgcttgtcct aaagatccag ccaaacctcc ggccaaggca caagttgtgg
     1981 gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa aaatcaagcg
     2041 gtggaccaga ggcagctgca ttcgtgaagg tatcaatgga cggagcacca tacttgagga
     2101 aaatcgattt gaggatgtat aagggaggtg gaggtggaga tatcgcagaa atcggtactg
     2161 gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg
     2221 ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg
     2281 tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga
     2341 tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct
     2401 tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact
     2461 ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg
     2521 catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg
     2581 agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg
     2641 tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg
     2701 accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa
     2761 acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg
     2821 actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga
     2881 tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca
     2941 tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg
     3001 cgcgctggct gtcgacgctc gagatttccg gctgaacgcg tactttatgc tgaagattct
     3061 tcaagttgat tcagtgtatt actgattttt ttctatttgt aaaggattat gagttgtata
     3121 aaatacatac tccctaaact agatcatgaa actggtttct gttttatgca gttgtcattt
     3181 gtaaagtcta ataaaatatt ctctataatt gcttctagat tacaaaaata tgacaatctt
     3241 gtaagtagca gactatggag aaaaatgagt tacctggagg gtcaggtaac ttgccaaact
     3301 aaaaagtatg ttagttgagg caaagtccta agcaaggttg tgctatcaag gctcagcata
     3361 ccttcgtggg cctttgattt accaacactg gaaatgcctg ccaactaatc ttggatagat
     3421 tctttaaggc attccactta gcttgccagt tgagacaatc accacagtta ttacccaaat
     3481 actatgaaca tatatttgta aaccagtcat tctgaattat agtgatgaga atttaaatat
     3541 atgcttttct agaatttgat gtttgaccat ttatgactta attaccagag agccagtaaa
     3601 ttaggacagt gtttcaacaa gcctaggcta tctcgtaagt tgaaaaatat cccactatag
     3661 ttgcttcatg agtatgaagt aagatggcct ctgatttaca ctggttcaat ttacaaattt
     3721 tcaactttat gataggttta tccgggtact aaatgcattt caacttgata gtttcaactt
     3781 atgataggtt taccaggatg tagtcccact gttgaggagc atctatttag gggttaatta
     3841 ctttagtaat aagtggaaag taagatacct tgagtaatgt ttgcctataa aattgtcagc
     3901 gtatttttac actattggct caagaatgtt ataatgctaa ggatccatca cactggcggc
     3961 cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat tacaattcac
     4021 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4081 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4141 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     4201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4501 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4561 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaattcaggg
     4621 cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa acggtgctga
     4681 ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga
     4741 aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca
     4801 gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa
     4861 gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat
     4921 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4981 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5041 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5101 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5161 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5221 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat cccaccttgc tcctgccgag
     5281 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5341 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5401 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5461 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5521 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5581 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5641 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5701 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattgaaaa aggaagagta
     5761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa ac
//
