LOCUS       1875                 9644 bp    DNA     circular SYN 21-FEB-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3582..6080
                     /note="pLannotate"
                     /label="Cas9(C)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1980..3581
                     /note="pLannotate"
                     /label="Cas9(N)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             8572..9432
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6558..7265
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        1051..1336
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     intron          1615..1842
                     /note="pLannotate"
                     /label="hybrid intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     repeat_region   7515..7655
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    7299..7410
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        8467..8571
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_RNA        879..954
                     /note="pLannotate"
                     /label="gRNA scaffold"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
     rep_origin      join(9603..9644,1..547)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      7730..8185
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1338..1614
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        609..849
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6141..6470
                     /note="pLannotate"
                     /label="GMNN (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="52.6"
                     /fragment="True"
                     /other="CDS"
     misc_RNA        961..1004
                     /note="pLannotate"
                     /label="gRNA scaffold (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="57.9"
                     /fragment="True"
                     /other="misc_RNA"
     misc_feature    complement(7660..7711)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(8186..8235)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(8337..8372)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
      661 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
      721 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      781 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      841 ggaaaggacg aaacaccgtg tccccattac tactcttggt tttagagcta gaaatagcaa
      901 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      961 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
     1021 attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc
     1081 tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt
     1141 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1201 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1261 ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1321 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     1381 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     1441 ggcggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     1501 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1561 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     1621 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     1681 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     1741 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
     1801 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
     1861 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
     1921 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
     1981 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     2041 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     2101 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     2161 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     2221 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     2281 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     2341 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     2401 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     2461 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     2521 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     2581 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     2641 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     2701 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     2761 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     2821 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     2881 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     2941 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     3001 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     3061 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     3121 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     3181 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     3241 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     3301 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     3361 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     3421 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     3481 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     3541 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     3601 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     3661 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     3721 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     3781 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     3841 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     3901 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     3961 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     4021 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     4081 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     4141 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     4201 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     4261 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     4321 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     4381 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     4441 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     4501 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     4561 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     4621 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     4681 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     4741 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     4801 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     4861 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     4921 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     4981 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     5041 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     5101 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     5161 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     5221 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     5281 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     5341 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     5401 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     5461 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     5521 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     5581 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     5641 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     5701 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     5761 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     5821 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     5881 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     5941 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     6001 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     6061 tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
     6121 agaaaaaggg atccaagctt atgaatccca gtatgaagca gaaacaagaa gaaatcaaag
     6181 agaatataaa gaatagttct gtcccaagaa gaactctgaa gatgattcag ccttctgcat
     6241 ctggatctct tgttggaaga gaaaatgagc tgtccgcagg cttgtccaaa aggaaacatc
     6301 ggaatgacca cttaacatct acaacttcca gccctggggt tattgtccca gaatctagtg
     6361 aaaataaaaa tcttggagga gtcacccagg agtcatttga tcttatgatt aaagaaaatc
     6421 catcctctca gtattggaag gaagtggcag aaaaacggag aaaggcgctg ggaagcggag
     6481 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctgaat
     6541 tcatgccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6601 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6661 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6721 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6781 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6841 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6901 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6961 cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc tccgagcgga
     7021 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     7081 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     7141 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     7201 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     7261 acaagtagga attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     7321 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     7381 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     7441 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     7501 tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     7561 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     7621 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     7681 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     7741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7801 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7921 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     7981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8041 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     8101 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8161 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     8221 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8281 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8341 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8401 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc
     9301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaac
//
