LOCUS       1916                 9407 bp    DNA     circular SYN 21-FEB-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(1263..2099)
                     /note="pLannotate"
                     /label="HA-R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(6548..7049)
                     /note="pLannotate"
                     /label="HA-L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(2647..3109)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(7775..8203)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2236..2634)
                     /note="pLannotate"
                     /label="BSD"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     intron          complement(3276..3505)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(4279..6003)
                     /note="pLannotate"
                     /label="OsTIR1"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        8230..8334
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(6249..6340)
                     /note="pLannotate"
                     /label="pause site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             8335..9195
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(2127..2208)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      join(9366..9407,1..547)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3598..4140)
                     /note="pLannotate"
                     /label="SNAP-tag®"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.5"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(6354..6402)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(6028..6239)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7676..7743
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.1"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4144..4254)
                     /note="pLannotate"
                     /label="13xMyc (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="22.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(727..857)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="11.0"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2214..2235)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
      781 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
      841 tcaagggcat cggtcgagcc cagagacagt gaccaaccat ccctgttttc ctaggactga
      901 gggtttcagc gctaaaacta ggctgtcctg ggcaaacagc ataagctggt caccccacac
      961 ccagacctga cccaaaccca gctcccctgc ttcttggcca cgtaacctga gaagggaatc
     1021 cctcctctct gaaccccagc ccaccccaat gctccaggcc tcctgggata ccccgaagag
     1081 tgagtttgcc aagcagtcac cccacagttg gaggagaatc cacccaaaag gcagcctggt
     1141 agacagggct ggggtggcct ctcgtggggt ccaggccaag taggtggcct ggggcctctg
     1201 ggggatgcag gggaaggggg atgcagggga acggggatgc aggggaacgg ggctcagtct
     1261 gaagagcaga gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg
     1321 tggacgagga agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa
     1381 acccagaagc ccagagcagg gccttaggga agcgggaccc tgctctgggc ggaggaatat
     1441 gtcccagata gcactgggga ctctttaagg aaagaaggat ggagaaagag aaagggagta
     1501 gaggcggcca cgacctggtg aacacctagg acgcaccatt ctcacaaagg gagttttcca
     1561 cacggacacc cccctcctca ccacagccct gccaggacgg ggctggctac tggccttatc
     1621 tcacaggtaa aactgacgca cggaggaaca atataaattg gggactagaa aggtgaagag
     1681 ccaaagttag aactcaggac caacttattc tgattttgtt tttccaaact gcttctcctc
     1741 ttgggaagtg taaggaagct gcagcaccag gatcagtgaa acgcaccaga cagccgcgtc
     1801 agagcagctc aggttctggg agagggtagc gcagggtggc cactgagaac cgggcaggtc
     1861 acgcatcccc cccttccctc ccaccccctg ccaagctctc cctcccagga tcctctctgg
     1921 ctccatcgta agcaaacctt agaggttctg gcaaggagag agatggctcc aggaaatggg
     1981 ggtgtgtcac cagataagga atctgcctaa caggaggtgg gggttagacc caatatcagg
     2041 agactaggaa ggaggaggcc taaggatggg gcttttctgt caccaatcct gtccctagtg
     2101 atgcatggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2161 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caccgataag
     2221 cttgatatcg aattcttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc
     2281 aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc
     2341 gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat
     2401 cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag
     2461 ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc
     2521 gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt
     2581 agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatggaagg
     2641 tcgtctttat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag
     2701 acgttttttt aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga
     2761 tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga
     2821 gagccatttg actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc
     2881 ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg
     2941 ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag
     3001 aggaactgct tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag
     3061 acccctagga atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa
     3121 agacggcaat atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg
     3181 gcttcggcca gtaacgttag gggggggggg gagaggggcg gaattggccg ccctagatgc
     3241 atgctcgacc tgcagttgga cctgggagtg gacacctgtg gagagaaagg caaagtggat
     3301 gtcattgtca ctcaagtgta tggccagatc tcaagcctgc cacacctcaa gcttgacaac
     3361 aaaaagattg tcttttctga ccagatggac gcggccaccc tcaaaggcat caccgcgggc
     3421 caggtgaata tcaaatcctc ctcgtttttg gaaactgaca atcttagcgc agaagtcatg
     3481 cccgcttttg agagggagta ctcaccccaa cagctggccc tcgcagacag cgaattaatt
     3541 cggcggccgg ttaacctaat caagtttgac ccgtttggct gcggctctag aaagcttacc
     3601 cagcccaggc ttgcccagtc tgtggccctc gtgggccagc agccactctt tcacggcgag
     3661 cccgccctcg tagcccccca cgtccaggtc gccctgcacc acccggtggc aggggatcag
     3721 aatgggcacg ggatttccgc tcagggcggt tttcacggcg gcggtggcgg cgggattgcc
     3781 ggccagggcg gccaggtggc tgtagctgat gacctctccg aacttcacca ctttcagcag
     3841 tttccacagc acctggcggg taaagctctc ctgctggaac actgggtggt gcagggctgg
     3901 cacagggaac tcctcgatgg cctcaggctg gtgaaagtag gcgttgagcc aggcggtggc
     3961 ctgcatcagt ggctctggtc cgcccagcac ggcggctggg gcaggcactt ccacggcgtc
     4021 ggcggcagat gttcctttgc ccaggaagat gatacggtgc aggccctgtt cgcacccaga
     4081 cagttccagc ttgcccagag ggctatccag ggtggtgcgc ttcatttcgc agtctttgtc
     4141 ggatccgttc aagtcttctt ctgagattaa tttttgttca ccgttcaagt cttcctcgga
     4201 gattagcttt tgttcaccgt tcaaatcttc ttcagaaatc aacttttgtt caccactagc
     4261 agcagaaccg gagtcgacta ggattttaac aaaatttggt gcatcatccc tcgccccagc
     4321 agttgtgcgg tacacatata atttctccac tttaggcaga tctccatggt tttcctccat
     4381 ttcattgcta ccatcccgct catttatgac ctcaacattg agcatcggca tctttgacgc
     4441 aaggacttgg caccccttta acgtgacatt gcaagatgac atccaaaggg atcgcattgt
     4501 ctcgtaccta gcaaaattcc ccaagagtgc agcatcacca aacgggctat ctcttatctc
     4561 cagtttcctt aaattcttgc atccattcat aacatgcatc atacccttat cactgtcacc
     4621 agcaaatgct atagaaagca tctcaagttg ttttgcatat ttcccaatat acatgaaaac
     4681 tttgtctgtg agaagaccag atattgacaa acgttgtaat cccttgcact cacgaacaat
     4741 agctccaaag ccttcatcta atggttggct tgtcacaaca tcaggcttcc ctggctcaag
     4801 aatacaaagt ctgaatcgtg tgaaatttgg acagttcttg gcgacagtaa ctagtgcagc
     4861 attagtcatt tggtgacaga agtacagtag tgagttcagt tttggacagc ccaaggatac
     4921 tgcaacaagt ccctcctctg tcactgcaga ataaccagct acgtagaaat ctgatggaaa
     4981 taccctgagt tcttgcaagt ctttgcaact ggaggccacc acttgcaagc ctttgtccga
     5041 gatacaatcc agtacccaaa ggcgttggag cttcacacag cggctaatca tttttgtaag
     5101 gtcagaagca tcaagtgtgg gtgcatagct caagttcaat cctgtcagtt gagcacacag
     5161 ggggtagata aatgacaggc aaacaggaga agcatcccag aatccagaca aactcctcaa
     5221 catcttgcat ttctccagag cactggtaag cttaaagtag gactcagttt ggaagtcatc
     5281 tgtcaaattc cctgtcccca aatcctccaa gttaggggta cgcagtagta tctttgcaag
     5341 tgtatctacc gatacagatc gattcagcct caaactccgc aggtttgggg acctgctaac
     5401 aagtctctcc agtgaaccag cattaacctc ccctttgatg caggcaaaat tcaatgagac
     5461 aagtgatgtg caggaatcag ggaagcagga aagccaccta ggccctcgat cctccacttc
     5521 attttcctgc aaatccaact ccctcagaag cttgcaatgg ctcgcgacgg cggctagccc
     5581 gtcagtgctg aacccctcgc agctgataag aacaagagcc ctgaaccgcg ggaacgagcg
     5641 agccagcagt tcgaggctct cgtcggacac caccatccgc ttcatcctga gctcctccag
     5701 gccgtggcat cccctcgcgg ccgcctcgat ccacggcccc gcgtagccgc cccagtcggg
     5761 gggcacgagg ttgaagtcgg cgccgtgggg cttccccttc accgtgagcg cccgcacgtt
     5821 ggggaaccgc gcggcgacgc ggccggcgcg cacggcgtag cagttgccca cgaagacgcc
     5881 gcggcggctc agcctctcga tctcgtacca caccttgcag acgagcgaga ccgtgttgcg
     5941 gtcgcgctgc gccggcagga agctgaagat gtgctccacc acctcctccg ggaagtacgt
     6001 catggtggaa gcgctctaga accggtcctg tgttctggcg gcaaacccgt tgcgaaaaag
     6061 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     6121 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccgga
     6181 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     6241 ctactagtag agaaatgttc tggcacctgc acttgcactg gggacagcct attttgctag
     6301 tttgttttgt ttcgttttgt tttgatggag agcgtatgtt agtactatcg attcacacaa
     6361 aaaaccaaca cacagatgta atgaaaataa agatatttta ttctcgaggt gataattcca
     6421 cgcatgcgcc ccactgtggg gtggagggga cagataaaag tacccagaac cagagccaca
     6481 ttaaccggcc ctgggaatat aaggtggtcc cagctcgggg acacaggatc cctggaggca
     6541 gcaaacatgc tgtcctgaag tggacatagg ggcccgggtt ggaggaagaa gactagctga
     6601 gctctcggac ccctggaaga tgccatgaca gggggctgga agagctagca cagactagag
     6661 aggtaagggg ggtaggggag ctgcccaaat gaaaggagtg agaggtgacc cgaatccaca
     6721 ggagaacggg gtgtccaggc aaagaaagca agaggatgga gaggtggcta aagccaggga
     6781 gacggggtac tttggggttg tccagaaaaa cggtgatgat gcaggcctac aagaagggga
     6841 ggcgggacgc aagggagaca tccgtcggag aaggccatcc taagaaacga gagatggcac
     6901 aggccccaga aggagaagga aaagggaacc cagcgagtga agacggcatg gggttgggtg
     6961 agggaggaga gatgcccgga gaggacccag acacggggag gatccgctca gaggacatca
     7021 cgtggtgcag cgccgagaag gaagtgctcc ggaaagagca tccttgggca gcaacacagc
     7081 agagagcaag gggaagaggg agtggaggaa gacggaacct gaaggaggcg gcagggaagg
     7141 atctgggcca gccgtagagg tgacccaggc cacaagctgc agacagaaag cggcacaggc
     7201 ccaggggaga gaatgcaggt cagagaaagc aggacctgcc tgggaagggg aaacagtggg
     7261 ccagaggcgg cgcagaagcc agtagagctc aaagtggtcc ggactcagga gagagacggc
     7321 agcgttagag ggcagagttc cggcggcaca gcaagggcac tcgggggcga gaggagggca
     7381 gcgcaaagtg acaatggcca gggccaggca gatagaccag actgagctat gggagctggc
     7441 tcaggttcag gagagggcag ggcagggaag gagacaaagt ccaggaccgg ctggaggggc
     7501 tcaacatcgg aagaggggaa gtcgagggag ggatggtaag gaggactgca tgggtcagca
     7561 caggctgcca aagccagggc cagttaaagc gactccaatg cggaagagag taggtcgacg
     7621 gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag cttgggctgg
     7681 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7741 gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7801 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7861 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7921 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7981 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8041 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8101 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8161 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     8221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaac
//
