LOCUS       Exported               11874 bp ds-DNA     circular SYN 10-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Jiang-An Yin
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2025 from SnapGene 5.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        386..661
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     misc_feature    1769..2674
                     /label=Dnmt3A
                     /note="color: #ffcc00"
     misc_feature    2756..3400
                     /label=Dnmt3L
                     /note="color: #99cc00"
     CDS             3641..7744
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /note="This feature has 5 segments:
                        1: 3641 .. 3667 / #993366
                        2: 3668 .. 3670 / #cc99b2
                        3: 3671 .. 6157 / #993366
                        4: 6158 .. 6160 / #cc99b2
                        5: 6161 .. 7744 / #993366"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     7642..7661
                     /label=dCas9 seq fwd
                     /note="color: black; sequence: ACGCTATACCTCCACCAAAG; added:
                     2022-11-01"
     CDS             7748..7774
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             7793..7813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7820..7840
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7880..8575
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarletI
                     /note="matures faster than mScarlet"
                     /note="color: #ff0000"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             8621..8806
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /note="color: #993366"
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     polyA_signal    8963..9018
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     primer_bind     complement(9377..9393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9401..9417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9425..9455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9425 .. 9431 / #ffffff / -10
                        2: 9432 .. 9449 / #ffffff
                        3: 9450 .. 9455 / #ffffff / -35"
     protein_bind    9470..9491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9550..9745
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      9596..9731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    9751..9885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(10124..10712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10883..11743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10883 .. 11674 / #ccffcc
                        2: 11675 .. 11743 / #ccffcc / signal sequence
                     Cleavage site after base 11674"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11744..11848)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gaaccatgac caggaatttg accccccaaa
     1801 ggtttaccca cctgtgccag ctgagaagag gaagcccatc cgcgtgctgt ctctctttga
     1861 tgggattgct acagggctcc tggtgctgaa ggacctgggc atccaagtgg accgctacat
     1921 tgcctccgag gtgtgtgagg actccatcac ggtgggcatg gtgcggcacc agggaaagat
     1981 catgtacgtc ggggacgtcc gcagcgtcac acagaagcat atccaggagt ggggcccatt
     2041 cgacctggtg attggaggca gtccctgcaa tgacctctcc attgtcaacc ctgcccgcaa
     2101 gggactttat gagggtactg gccgcctctt ctttgagttc taccgcctcc tgcatgatgc
     2161 gcggcccaag gagggagatg atcgcccctt cttctggctc tttgagaatg tggtggccat
     2221 gggcgttagt gacaagaggg acatctcgcg atttcttgag tctaaccccg tgatgattga
     2281 cgccaaagaa gtgtctgctg cacacagggc ccgttacttc tggggtaacc ttcctggcat
     2341 gaacaggcct ttggcatcca ctgtgaatga taagctggag ctgcaagagt gtctggagca
     2401 cggcagaata gccaagttca gcaaagtgag gaccattacc accaggtcaa actctataaa
     2461 gcagggcaaa gaccagcatt tccccgtctt catgaacgag aaggaggaca tcctgtggtg
     2521 cactgaaatg gaaagggtgt ttggcttccc cgtccactac acagacgtct ccaacatgag
     2581 ccgcttggcg aggcagagac tgctgggccg atcgtggagc gtgccggtca tccgccacct
     2641 cttcgctccg ctgaaggaat attttgcttg tgtgtctagc ggcaatagta acgctaacag
     2701 ccgcgggccg agcttcagca gcggcctggt gccgttaagc ttgcgcggca gccatatggg
     2761 ccctatggag atatacaaga cagtgtctgc atggaagaga cagccagtgc gggtactgag
     2821 cctcttcaga aacatcgaca aggtactaaa gagtttgggc ttcttggaaa gcggttctgg
     2881 ttctggggga ggaacgctga agtacgtgga agatgtcaca aatgtcgtga ggagagacgt
     2941 ggagaaatgg ggcccctttg acctggtgta cggctcgacg cagcccctag gcagctcttg
     3001 tgatcgctgt cccggctggt acatgttcca gttccaccgg atcctgcagt atgcgctgcc
     3061 tcgccaggag agtcagcggc ccttcttctg gatattcatg gacaatctgc tgctgactga
     3121 ggatgaccaa gagacaacta cccgcttcct tcagacagag gctgtgaccc tccaggatgt
     3181 ccgtggcaga gactaccaga atgctatgcg ggtgtggagc aacattccag ggctgaagag
     3241 caagcatgcg cccctgaccc caaaggaaga agagtatctg caagcccaag tcagaagcag
     3301 gagcaagctg gacgccccga aagttgacct cctggtgaag aactgccttc tcccgctgag
     3361 agagtacttc aagtattttt ctcaaaactc acttcctctt ggagggccga gctctggcgc
     3421 acccccacca agtggagggt ctcctgccgg gtccccaaca tctactgaag aaggcaccag
     3481 cgaatccgca acgcccgagt caggccctgg tacctccaca gaaccatctg aaggtagtgc
     3541 gcctggttcc ccagctggaa gccctacttc caccgaagaa ggcacgtcaa ccgaaccaag
     3601 tgaaggatct gcccctggga ccagcactga accatctgag atggacaaga agtattctat
     3661 cggactggcc atcgggacta atagcgtcgg gtgggccgtg atcactgacg agtacaaggt
     3721 gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca agaaaaatct
     3781 gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc tcaagcggac
     3841 tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag agatattctc
     3901 caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat cattcctcgt
     3961 ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg acgaggtggc
     4021 ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg actcaaccga
     4081 caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt tcagaggcca
     4141 cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac tgttcatcca
     4201 gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa gcggagtcga
     4261 tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga atcttatcgc
     4321 tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc tttcacttgg
     4381 gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc tgcaactgtc
     4441 caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg accaatacgc
     4501 cgaccttttc cttgctgcta agaatctttc tgacgccatc ctgctgtctg acattctccg
     4561 cgtgaacact gaaatcacca aggcccctct ttcagcttca atgattaagc ggtatgatga
     4621 gcaccaccag gacctgaccc tgcttaaggc actcgtccgg cagcagcttc cggagaagta
     4681 caaggaaatc ttctttgacc agtcaaagaa tggatacgcc ggctacatcg acggaggtgc
     4741 ctcccaagag gaattttata agtttatcaa acctatcctt gagaagatgg acggcaccga
     4801 agagctcctc gtgaaactga atcgggagga tctgctgcgg aagcagcgca ctttcgacaa
     4861 tgggagcatt ccccaccaga tccatcttgg ggagcttcac gccatccttc ggcgccaaga
     4921 ggacttctac ccctttctta aggacaacag ggagaagatt gagaaaattc tcactttccg
     4981 catcccctac tacgtgggac ccctcgccag aggaaatagc cggtttgctt ggatgaccag
     5041 aaagtcagaa gaaactatca ctccctggaa cttcgaagag gtggtggaca agggagccag
     5101 cgctcagtca ttcatcgaac ggatgactaa cttcgataag aacctcccca atgagaaggt
     5161 cctgccgaaa cattccctgc tctacgagta ctttaccgtg tacaacgagc tgaccaaggt
     5221 gaaatatgtc accgaaggga tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc
     5281 aattgtggac cttctgttca agaccaatag aaaggtgacc gtgaagcagc tgaaggagga
     5341 ctatttcaag aaaattgaat gcttcgactc tgtggagatt agcggggtcg aagatcggtt
     5401 caacgcaagc ctgggtacct accatgatct gcttaagatc atcaaggaca aggattttct
     5461 ggacaatgag gagaacgagg acatccttga ggacattgtc ctgactctca ctctgttcga
     5521 ggaccgggaa atgatcgagg agaggcttaa gacctacgcc catctgttcg acgataaagt
     5581 gatgaagcaa cttaaacgga gaagatatac cggatgggga cgccttagcc gcaaactcat
     5641 caacggaatc cgggacaaac agagcggaaa gaccattctt gatttcctta agagcgacgg
     5701 attcgctaat cgcaacttca tgcaacttat ccatgatgat tccctgacct ttaaggagga
     5761 catccagaag gcccaagtgt ctggacaagg tgactcactg cacgagcata tcgcaaatct
     5821 ggctggttca cccgctatta agaagggtat tctccagacc gtgaaagtcg tggacgagct
     5881 ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc atcgagatgg ccagggaaaa
     5941 ccagactacc cagaagggac agaagaacag cagggagcgg atgaaaagaa ttgaggaagg
     6001 gattaaggag ctcgggtcac agatccttaa agagcacccg gtggaaaaca cccagcttca
     6061 gaatgagaag ctctatctgt actaccttca aaatggacgc gatatgtatg tggaccaaga
     6121 gcttgatatc aacaggctct cagactacga cgtggacgcc atcgtccctc agagcttcct
     6181 caaagacgac tcaattgaca ataaggtgct gactcgctca gacaagaacc ggggaaagtc
     6241 agataacgtg ccctcagagg aagtcgtgaa aaagatgaag aactattggc gccagcttct
     6301 gaacgcaaag ctgatcactc agcggaagtt cgacaatctc actaaggctg agaggggcgg
     6361 actgagcgaa ctggacaaag caggattcat taaacggcaa cttgtggaga ctcggcagat
     6421 tactaaacat gtcgcccaaa tccttgactc acgcatgaat accaagtacg acgaaaacga
     6481 caaacttatc cgcgaggtga aggtgattac cctgaagtcc aagctggtca gcgatttcag
     6541 aaaggacttt caattctaca aagtgcggga gatcaataac tatcatcatg ctcatgacgc
     6601 atatctgaat gccgtggtgg gaaccgccct gatcaagaag tacccaaagc tggaaagcga
     6661 gttcgtgtac ggagactaca aggtctacga cgtgcgcaag atgattgcca aatctgagca
     6721 ggagatcgga aaggccaccg caaagtactt cttctacagc aacatcatga atttcttcaa
     6781 gaccgaaatc acccttgcaa acggtgagat ccggaagagg ccgctcatcg agactaatgg
     6841 ggagactggc gaaatcgtgt gggacaaggg cagagatttc gctaccgtgc gcaaagtgct
     6901 ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa
     6961 ggaatcaatc ctccccaagc gcaactccga caagctcatt gcaaggaaga aggattggga
     7021 ccctaagaag tacggcggat tcgattcacc aactgtggct tattctgtcc tggtcgtggc
     7081 taaggtggaa aaaggaaagt ctaagaagct caagagcgtg aaggaactgc tgggtatcac
     7141 cattatggag cgcagctcct tcgagaagaa cccaattgac tttctcgaag ccaaaggtta
     7201 caaggaagtc aagaaggacc ttatcatcaa gctcccaaag tatagcctgt tcgaactgga
     7261 gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt cagaagggta atgagctggc
     7321 tctcccctcc aagtacgtga atttcctcta ccttgcaagc cattacgaga agctgaaggg
     7381 gagccccgag gacaacgagc aaaagcaact gtttgtggag cagcataagc attatctgga
     7441 cgagatcatt gagcagattt ccgagttttc taaacgcgtc attctcgctg atgccaacct
     7501 cgataaagtc cttagcgcat acaataagca cagagacaaa ccaattcggg agcaggctga
     7561 gaatatcatc cacctgttca ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt
     7621 cgacaccacc atcgaccgga aacgctatac ctccaccaaa gaagtgctgg acgccaccct
     7681 catccaccag agcatcaccg gactttacga aactcggatt gacctctcac agctcggagg
     7741 ggatgcctat ccctatgacg tgcccgatta tgccagcctg ggcagcggct cccccaagaa
     7801 aaaacgcaag gtggaagatc ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag
     7861 caacggcagc agcggatcca tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg
     7921 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     7981 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     8041 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcat
     8101 caagcacccc gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg
     8161 ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct
     8221 ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg
     8281 ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga
     8341 ggacggcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta
     8401 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta
     8461 caacgtcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca
     8521 gtacgaacgc tccgagggcc gccactccac cggcggcatg gacgagctgt acaagggcgg
     8581 tggcggaggg atggatgcta agtcactaac tgcctggtcc cggacactgg tgaccttcaa
     8641 ggatgtattt gtggacttca ccagggagga gtggaagctg ctggacactg ctcagcagat
     8701 cgtgtacaga aatgtgatgc tggagaacta taagaacctg gtttccttgg gttatcagct
     8761 tactaagcca gatgtgatcc tccggttgga gaagggagaa gagccctagt aagcggccgc
     8821 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     8881 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     8941 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     9001 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     9061 aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc atgaacaaag
     9121 gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     9181 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     9241 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     9301 actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc tgcagcccaa
     9361 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9421 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9481 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9541 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9661 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9721 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     9781 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9841 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     9901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
    10321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10561 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//
