LOCUS       MZB131_pLann        7244 bp DNA     circular SYN 26-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   UNC Chapel Hill
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        208..226
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    227..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             265..287
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             337..630
                     /label=SMT3
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.0"
                     /note="/other=CDS"
     CDS             631..1521
                     /label=HaloTag(R)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             1537..1560
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1561..2262
                     /label=AP3D1
                     /label=AP3D1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=94.9"
                     /note="/match_length=21.8"
                     /note="/other=CDS"
     terminator      2438..2485
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      2521..2973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2999..3055
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.3"
                     /note="/match_length=56.3"
                     /note="/other=promoter"
     CDS             complement(3064..3873)
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      3994..4577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4762..4900)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.6"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(5001..5191)
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             5231..5341
                     /label=mbeC (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.2"
                     /note="/other=CDS"
     CDS             complement(5341..5487)
                     /label=YPB2_ECOLX (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=89.8"
                     /note="/match_length=56.3"
                     /note="/other=CDS"
     CDS             complement(5656..5945)
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=24.5"
                     /note="/other=CDS"
     CDS             complement(5993..7067)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7068..7144)
                     /label=lacI promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(join(7152..7244,1..186))
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc atggcagaaa tcggtactgg ctttccattc
      661 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
      721 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
      781 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
      841 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
      901 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
      961 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     1021 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     1081 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     1141 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     1201 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     1261 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     1321 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     1381 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     1441 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     1501 tcgacgctcg agatttccgg cgagccaacc actggcctgg aagtgctgtt tcagggcccg
     1561 tcaagctact cattgcttgc tgaaaacagc tacgtaaaga tgacttgtga catccggggc
     1621 tccttgcaag aggattcgca ggtcaccgtg gcaatcgtat tggagaaccg ctcgtcatcg
     1681 attttgaaag gtatggaatt gagtgtttta gactctctga acgcgcgcat ggcacgtccc
     1741 cagggcagct ctgtacatga tggtgtgccc gtcccattcc aactgccccc cggagtatcg
     1801 aatgaggccc aatacgtatt tacgattcag tcaatcgtaa tggcgcaaaa gttgaaagga
     1861 acattgtctt ttatagcaaa aaatgacgag ggagcgaccc atgagaaatt ggatttccgt
     1921 ttgcatttct cttgctcatc ctatctgatt acgacccctt gttactctga cgcctttgcg
     1981 aagctgttgg agagtggaga tttgagtatg agcagtatta aagtcgatgg gatacggatg
     2041 tcattccaga accttttagc caagatctgc ttccaccatc atttttccgt tgtggagcgt
     2101 gttgactcct gcgcttcaat gtatagtcgt tcaatacaag gacatcacgt atgtttgctg
     2161 gtgaaaaaag gtgaaaattc tgtttccgta gatggaaaat gttctgactc tacactgtta
     2221 agtaatttat tagaagaaat gaaggctact ttggcaaaat gctaataagg atccatgggt
     2281 accctcgagt gaagacaagc ttaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     2341 ccaacttagt cgagcaccac caccaccacc actgagtccg gctgctaaca aagcccgaaa
     2401 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     2461 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact ataccggatt ggcgaatggg
     2521 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2581 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc ttctcgccac
     2641 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2701 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtggcca
     2761 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2821 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2881 gggatttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2941 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     3001 cggaacccct attgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     3061 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     3121 aataccatat ttttgaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     3181 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     3241 cctattaatt tcccctcgtc aaaaaaaggt tatcaagtga gaaatcacca tgagtgacga
     3301 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     3361 agccattacg ctcgtcatca aaatcactcg cacaaccaaa ccgttattca ttcgtgattg
     3421 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     3481 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaatt tttcacctga atcaggatat
     3541 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     3601 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattcgtc agccagttta
     3661 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     3721 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgccgacatt
     3781 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     3841 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     3901 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4021 ctgctgctgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4141 cttctagtgt agcctagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4261 ggttggactc aagacgatag taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4381 gctatgagaa agcgccacgc ttcccgaagg agaaaggcgg acaggtatcc ggtaagcggc
     4441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4501 agtcctgtcg ggtttcgcca cctctgactt gagcgcgatt tttgtgatgc tcgtcagggg
     4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga tttgtggata accgtattac
     4681 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4741 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatt gtgcggtatt
     4801 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4861 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaaaccc
     4921 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4981 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     5041 ctgggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     5101 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     5161 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggattctgtt
     5221 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     5281 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     5341 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     5401 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     5461 gacttccggt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     5521 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     5581 tctgctaacc agtaagcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     5641 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     5701 ggtggcggga ccagtgacga agcttgagcg agggcgtgca agattccgaa taccgcaagc
     5761 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     5821 gctgccggca cctgtcctac gagttgcata taaagaagac agtcataagt gcggcgacga
     5881 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     5941 gtcgagatcc cggtgcctaa tgagtgagct aacttaatta attgcgttgc gctcactgcc
     6001 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6061 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttaccagtg agacgggcaa
     6121 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     6181 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat acatgagctg
     6241 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     6301 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggaa
     6361 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     6421 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     6481 gacgagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     6541 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     6601 gttgatgggt gctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     6661 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     6721 tgcgcgagaa gattgtgccc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     6781 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     6841 acggcgcgtg cagggccaga ctggagtggc aacgccaatc agcaacgact gtttgcccgc
     6901 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     6961 ttcccgcgtt ttcgcagaaa cgtggctggc ctgttcacca cgcgggaaac ggtctgataa
     7021 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     7081 aattgactct cttccgggcg ctatcatgcc ataccgcgaa ggttttgcgc cattcgatgg
     7141 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     7201 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catg
//
