LOCUS       MZB150_pLann        6197 bp DNA     circular SYN 25-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6197
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     source          631..1215
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        208..226
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    227..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             265..287
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             337..630
                     /label=SMT3
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=CDS"
     CDS             631..1215
                     /codon_start=1
                     /label=CYTH2
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="ANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEI
                     ARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPG
                     EAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFV
                     AMNRGINEGGDLPEELLRNLYDSIRNEPFKIP"
     terminator      1391..1438
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      1474..1926
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        1952..2008
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.3"
                     /note="/match_length=56.3"
                     /note="/other=promoter"
     CDS             complement(2017..2826)
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(2931..3545)
                     /label=PBR322 Origin
                     /label=PBR322_Origin
                     /note="/vntifkey=21"
     misc_feature    complement(3715..3853)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.6"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(3954..4144)
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             4184..4294
                     /label=mbeC (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.2"
                     /note="/other=CDS"
     CDS             complement(4294..4440)
                     /label=YPB2_ECOLX (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=89.8"
                     /note="/match_length=56.3"
                     /note="/other=CDS"
     CDS             complement(4609..4898)
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=24.5"
                     /note="/other=CDS"
     CDS             complement(4946..6020)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(6021..6097)
                     /gene="lacI"
                     /label=lacI promoter
     CDS             complement(join(6105..6197,1..186))
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc gccaatgagg gcagtaagac cttgcaacgg
      661 aaccggaaga tggcaatggg caggaagaag ttcaacatgg accccaagaa ggggatccag
      721 ttcttggtgg agaatgaact gctgcagaac acacccgagg agatcgcccg cttcctgtac
      781 aagggcgagg ggctgaacaa gacagccatc ggggactacc tgggggagag ggaagaactg
      841 aacctggcag tgctccacgc ttttgtggat ctgcatgagt tcaccgacct caatctggtg
      901 caggccctca ggcagtttct atggagcttt cgcctacccg gagaggccca gaaaattgac
      961 cggatgatgg aggccttcgc ccagcgatac tgcctgtgca accctggggt tttccagtcc
     1021 acagacacgt gctatgtgct gtccttcgcc gtcatcatgc tcaacaccag tctccacaat
     1081 cccaatgtcc gggacaagcc gggcctggag cgctttgtgg ccatgaaccg gggcatcaac
     1141 gagggcgggg acctgcctga ggagctgctc aggaacctgt acgacagcat ccgaaatgag
     1201 cccttcaaga ttccttaata aggatccatg ggtaccctcg agtgaagaca agcttaggta
     1261 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt agtcgagcac caccaccacc
     1321 accactgagt ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1381 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1441 gaaaggagga actataccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     1501 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1561 ctcctttcgc tttcttccct tccttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1681 acttgattag ggtgatggtt cacgtagtgg ccatcgccct gatagacggt ttttcgccct
     1741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1801 aaccctatct cggtctattc ttttgattta taagggattt gccgatttcg gcctattggt
     1861 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     1921 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctattgttt atttttctaa
     1981 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     2041 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aagccgtttc
     2101 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2161 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaaaa
     2221 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     2281 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     2341 tcgcacaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     2401 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     2461 cgcatcaaca atttttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     2521 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     2581 gtcggaagag gcataaattc gtcagccagt ttagtctgac catctcatct gtaacatcat
     2641 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2701 atcgatagat tgtcgcacct gattgccgac attatcgcga gcccatttat acccatataa
     2761 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     2821 gctcataaca ccccttgtat tactgtttat gtagcagaca gttttattgt tcatgaccaa
     2881 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2941 atcttcttga gatccttttt ttctgcgcgt aatctgctgc tgcaaacaaa aaaaccaccg
     3001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3061 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagcctag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagtaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 aggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3481 cttgagcgcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3601 cgttatcccc tgatttgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3661 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     3721 gcggtatttt ctccttacgc attgtgcggt atttcacacc gcaatggtgc actctcagta
     3781 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     3841 ggtcatggct gcgccccgac acccgccaaa cccgctgacg cgccctgacg ggcttgtctg
     3901 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3961 ttttcaccgt catcaccgaa acgcgcgagg cagctgggta aagctcatca gcgtggtcgt
     4021 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     4081 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     4141 tcactgatgc ctccgtgtaa gggggattct gttcatgggg gtaatgatac cgatgaaacg
     4201 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     4261 tgagggtaaa caactggcgg tatggatgcg gcggaccaga gaaaaatcac tcagggtcaa
     4321 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     4381 atgcagatcc ggaacataat ggtgcagggc gctgacttcc ggtttccaga ctttacgaaa
     4441 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     4501 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagc aaccccgcca
     4561 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc
     4621 ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaagcttga
     4681 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     4741 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     4801 atataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     4861 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     4921 gctaacttaa ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     5041 gggtggtttt tcttttacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     5101 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     5161 gatggtggtt aacggcggga tatacatgag ctgtcttcgg tatcgtcgta tcccactacc
     5221 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5281 atctgatcgt tggcaaccag catcgcagtg gaacgatgcc ctcattcagc atttgcatgg
     5341 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     5401 gattgcgagt gagatattta tgccagccag ccagacgaga cgcgccgaga cagaacttaa
     5461 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     5521 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgctggtc agagacatca
     5581 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     5641 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg cccgccgctt
     5701 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     5761 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagt
     5821 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     5881 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     5941 ggcctgttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     6001 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     6061 gccataccgc gaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
     6121 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     6181 gcaaggaatg gtgcatg
//
