LOCUS       MZB207_pLann        6377 bp DNA     circular SYN 25-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6377
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        208..226
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    227..251
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     RBS             265..287
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             337..633
                     /label=SMT3 (SUMO)
                     /label=SMT3
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=CDS"
     CDS             join(679..681,682..684,685..1395)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      1571..1618
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      1654..2106
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        2132..2188
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.3"
                     /note="/match_length=56.3"
                     /note="/other=promoter"
     CDS             complement(2197..3006)
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(3111..3725)
                     /label=PBR322 Origin
                     /label=PBR322_Origin
                     /note="/vntifkey=21"
     misc_feature    complement(3895..4033)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.6"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(4134..4324)
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             4364..4474
                     /label=mbeC (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.2"
                     /note="/other=CDS"
     CDS             complement(4474..4620)
                     /label=YPB2_ECOLX (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=89.8"
                     /note="/match_length=56.3"
                     /note="/other=CDS"
     CDS             complement(4789..5078)
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=24.5"
                     /note="/other=CDS"
     CDS             complement(5126..6200)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(6201..6277)
                     /gene="lacI"
                     /label=lacI promoter
     CDS             complement(join(6285..6377,1..186))
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc gcgctgaaaa tggtgaaagg cagcattgat
      661 cgcatgtttg ataaaggcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc
      781 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      841 cccgtgccct ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc
      901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      961 caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag
     1021 ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac
     1081 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg
     1141 gccgacaagc agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac
     1201 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1261 ctgctgcccg acaaccacta cctgagccat cagtccaagc tgagcaaaga ccccaacgag
     1321 aagcgcgatc acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg
     1381 gacgagctgt acaagtaata aggatccatg ggtaccctcg agtgaagaca agcttaggta
     1441 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt agtcgagcac caccaccacc
     1501 accactgagt ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1561 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1621 gaaaggagga actataccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     1681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1741 ctcctttcgc tttcttccct tccttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1801 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1861 acttgattag ggtgatggtt cacgtagtgg ccatcgccct gatagacggt ttttcgccct
     1921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1981 aaccctatct cggtctattc ttttgattta taagggattt gccgatttcg gcctattggt
     2041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     2101 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctattgttt atttttctaa
     2161 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     2221 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aagccgtttc
     2281 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2341 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaaaa
     2401 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     2461 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     2521 tcgcacaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     2581 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     2641 cgcatcaaca atttttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     2701 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     2761 gtcggaagag gcataaattc gtcagccagt ttagtctgac catctcatct gtaacatcat
     2821 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2881 atcgatagat tgtcgcacct gattgccgac attatcgcga gcccatttat acccatataa
     2941 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     3001 gctcataaca ccccttgtat tactgtttat gtagcagaca gttttattgt tcatgaccaa
     3061 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3121 atcttcttga gatccttttt ttctgcgcgt aatctgctgc tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagcctag ttaggccacc
     3301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagtaccgga
     3421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3541 aggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3661 cttgagcgcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3781 cgttatcccc tgatttgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3841 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     3901 gcggtatttt ctccttacgc attgtgcggt atttcacacc gcaatggtgc actctcagta
     3961 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     4021 ggtcatggct gcgccccgac acccgccaaa cccgctgacg cgccctgacg ggcttgtctg
     4081 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4141 ttttcaccgt catcaccgaa acgcgcgagg cagctgggta aagctcatca gcgtggtcgt
     4201 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     4261 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     4321 tcactgatgc ctccgtgtaa gggggattct gttcatgggg gtaatgatac cgatgaaacg
     4381 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     4441 tgagggtaaa caactggcgg tatggatgcg gcggaccaga gaaaaatcac tcagggtcaa
     4501 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     4561 atgcagatcc ggaacataat ggtgcagggc gctgacttcc ggtttccaga ctttacgaaa
     4621 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     4681 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagc aaccccgcca
     4741 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc
     4801 ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaagcttga
     4861 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     4921 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     4981 atataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     5041 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     5101 gctaacttaa ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     5221 gggtggtttt tcttttacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     5281 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     5341 gatggtggtt aacggcggga tatacatgag ctgtcttcgg tatcgtcgta tcccactacc
     5401 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     5461 atctgatcgt tggcaaccag catcgcagtg gaacgatgcc ctcattcagc atttgcatgg
     5521 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     5581 gattgcgagt gagatattta tgccagccag ccagacgaga cgcgccgaga cagaacttaa
     5641 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     5701 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgctggtc agagacatca
     5761 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     5821 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg cccgccgctt
     5881 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     5941 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagt
     6001 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     6061 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     6121 ggcctgttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     6181 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     6241 gccataccgc gaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
     6301 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     6361 gcaaggaatg gtgcatg
//
