LOCUS       MZB208_pLann        6383 bp DNA     circular SYN 25-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6383
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        208..226
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    227..251
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     RBS             265..287
                     /label=RBS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=RBS"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             337..630
                     /label=SMT3 (SUMO)
                     /label=SMT3
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.0"
                     /note="/other=CDS"
     CDS             join(685..687,688..690,691..1401)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      1577..1624
                     /label=T7 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     rep_origin      1660..2112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2138..2194
                     /label=AmpR promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.3"
                     /note="/match_length=56.3"
                     /note="/other=promoter"
     CDS             complement(2203..3012)
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(3117..3731)
                     /label=PBR322 Origin
                     /label=PBR322_Origin
                     /note="/vntifkey=21"
     misc_feature    complement(3901..4039)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.6"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(4140..4330)
                     /label=rop
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             4370..4480
                     /label=mbeC (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.2"
                     /note="/other=CDS"
     CDS             complement(4480..4626)
                     /label=YPB2_ECOLX (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=89.8"
                     /note="/match_length=56.3"
                     /note="/other=CDS"
     CDS             complement(4795..5084)
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=24.5"
                     /note="/other=CDS"
     CDS             complement(5132..6206)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(6207..6283)
                     /label=lacI promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(join(6291..6383,1..186))
                     /label=TcR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.6"
                     /note="/match_length=23.5"
                     /note="/other=CDS"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      181 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      241 ataacaattc cccttagaaa taattttgtt taactttaag aaggagatat acatatgggc
      301 catcaccatc accatcacca tcaccatcac ggcagcatga gcgatagcga agtgaaccag
      361 gaagcgaaac cggaagtgaa accggaagtg aaaccggaaa cccatattaa cctgaaagtg
      421 agcgatggca gcagcgaaat tttttttaaa attaaaaaaa ccaccccgct gcgtcgtctg
      481 atggaagcgt ttgcgaaacg tcagggcaaa gaaatggata gcctgcgttt tctgtatgat
      541 ggcattcgta ttcaggcgga tcagaccccg gaagatctgg atatggaaga taacgatatt
      601 attgaagcgc atcgtgaaca gattggcggc aaaccgccga ccattctgcg cagcgtggtg
      661 aacagcatta ccggcagcag cggcatggtg agcaagggcg aggagctgtt caccggggtg
      721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtccgcggc
      781 gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc
      841 aagctgcccg tgccctggcc caccctcgtg accaccttag gctacggcgt ggcctgcttc
      901 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      961 tacgtccagg agcgcaccat ctctttcaag gacgacggta cctacaagac ccgcgccgag
     1021 gtgaagttcg agggcgacac cctggtgaac cgcatcgtgc tgaagggcat cgacttcaag
     1081 gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca caaggtctat
     1141 atcacggccg acaagcagaa gaacggcatc aaggctaact tcaagacccg ccacaacgtt
     1201 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1261 cccgtgctgc tgcccgacaa ccactacctg agccatcagt ccaagctgag caaagacccc
     1321 aacgagaagc gcgatcacat ggtcctgaag gagagggtga ccgccgccgg gattacacat
     1381 gacatggacg agctgtacaa gtaataagga tccatgggta ccctcgagtg aagacaagct
     1441 taggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gagcaccacc
     1501 accaccacca ctgagtccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     1561 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     1621 tttgctgaaa ggaggaacta taccggattg gcgaatggga cgcgccctgt agcggcgcat
     1681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1741 cgcccgctcc tttcgctttc ttcccttcct tctcgccacg ttcgccggct ttccccgtca
     1801 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1861 caaaaaactt gattagggtg atggttcacg tagtggccat cgccctgata gacggttttt
     1921 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1981 acactcaacc ctatctcggt ctattctttt gatttataag ggatttgccg atttcggcct
     2041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2101 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta ttgtttattt
     2161 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
     2221 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaagc
     2281 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2341 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2401 aaaaaaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     2461 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     2521 aatcactcgc acaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     2581 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     2641 tgccagcgca tcaacaattt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2701 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2761 ttgatggtcg gaagaggcat aaattcgtca gccagtttag tctgaccatc tcatctgtaa
     2821 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2881 catacaatcg atagattgtc gcacctgatt gccgacatta tcgcgagccc atttataccc
     2941 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     3001 aatatggctc ataacacccc ttgtattact gtttatgtag cagacagttt tattgttcat
     3061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgctgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gcctagttag
     3301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3541 tcccgaagga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3661 ctctgacttg agcgcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3781 ttcctgcgtt atcccctgat ttgtggataa ccgtattacc gcctttgagt gagctgatac
     3841 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3901 cctgatgcgg tattttctcc ttacgcattg tgcggtattt cacaccgcaa tggtgcactc
     3961 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     4021 tgactgggtc atggctgcgc cccgacaccc gccaaacccg ctgacgcgcc ctgacgggct
     4081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4141 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgggtaaagc tcatcagcgt
     4201 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     4261 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     4321 gtttggtcac tgatgcctcc gtgtaagggg gattctgttc atgggggtaa tgataccgat
     4381 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     4441 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg accagagaaa aatcactcag
     4501 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     4561 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccggtt tccagacttt
     4621 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     4681 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaagcaacc
     4741 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     4801 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     4861 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     4921 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     4981 agttgcatat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     5041 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     5101 gagtgagcta acttaattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5221 gcgccagggt ggtttttctt ttaccagtga gacgggcaac agctgattgc ccttcaccgc
     5281 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     5341 ctgtttgatg gtggttaacg gcgggatata catgagctgt cttcggtatc gtcgtatccc
     5401 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
     5461 agcgccatct gatcgttggc aaccagcatc gcagtggaac gatgccctca ttcagcattt
     5521 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     5581 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgagacgcg ccgagacaga
     5641 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
     5701 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg ctggtcagag
     5761 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
     5821 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcccg
     5881 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     5941 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     6001 tggagtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     6061 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     6121 gtggctggcc tgttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     6181 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     6241 tatcatgcca taccgcgaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
     6301 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     6361 gccgccgcaa ggaatggtgc atg
//
