LOCUS       Exported               16052 bp ds-DNA     circular SYN 12-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Tian
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 14, 2020 from SnapGene Viewer 5.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     protein_bind    complement(4987..5020)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="loxP mutant. Cre-mediated recombination occurs in 
                     the 8-bp core sequence (GCATACAT)."
     CDS             complement(5021..5644)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    5645..5728
                     /label=ptetO
     promoter        5663..5718
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    5669..5687
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5699..5717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5707..5755
                     
     misc_feature    5707..5729
                     /label=HA
     primer_bind     complement(5710..5729)
                     /label=spec-ptet-R
     CDS             5730..6707
                     /codon_start=1
                     /label=dehydrogenase
                     /translation="MSEKIYYWSPIKHWEKLHNEVLIGEMRFTGILSEWFPDFYFMAQK
                     GVKISELVERFSLGNVEETQKNIELMIKNRVLVSNILHPREVFSSQEKIFSNPYSNQIR
                     FSKEKLDKYMSKQLNRTHVAARSTEIQLETTNELPTIIKERRSCRQFDMEKHISFSEFS
                     RFLSTLKQVREEKIYYHYASAGGLYPIDIFVYVKPKRIEDMKAGFYYYNPSKNSLVIVN
                     NIDQVIKSDHELINQDLFTQSAFSVYLVYNANASIPKYGSDGYLFACIESGIITATLNM
                     VAETLNLGVCSVGHMKFEDIQQFLCLDNHQVFLHGLEVGLKINE"
     CDS             6740..13756
                     /codon_start=1
                     /label=NRPS
                     /translation="MHIKDRSSIHENKEVEAFWKEQLSSEILDFSVFKFNNDYQLNKET
                     PCEYVQVIVNVEFSKSIQKISKSQDLLLFSWFQAALRVCLAHYTDQERITIGIPIIEKN
                     LEQSENLNKLIPLGSEIQPHHTFKQLVNEIEQTTLFGYENQEYPLSELLVKHNLTPFQI
                     VIGMEGLHNENSLEEYAENNLALWIRRKWNEIHNEFEFQISCKSRSFSSLQQFANSYCY
                     VLKQVSQNPNLALKDICIIAEEEKELLEGLNTFQTGIDLFQSFQDLFEAQVLKTPDQIA
                     VVCNDQSLTYRELNKKANQLASILQSKGVSKESIIGVMVDRSLEMIIGMMGILKAGAAY
                     LPIDPNYPTERIEYMLQDSQAKYLLSKRTEEVLPQFAGEVLYLDKEYLFQGEESNLVRE
                     HNPNDLAYVIYTSGSTGNPKGVMIEQKSLVHFLNVMREKVKANQSFLFLASVSFDISLL
                     EICLPLTQGSKVVIATEEQFATKELAHLVKKHKVDLWESTPSRMEVILSDPEGANFLKD
                     LKSILLAGEAFSIDLVEKIRCISEATILNIYGPTETTICATVKDLSTSKEVTIGKPNPN
                     YHSYILNKYGQLKPFGIPGELCIAGVAVARGYLGKSKLTDEKFVQSPFAAGEMMYHTGD
                     LVRWLPNGELEYLGRMDHQVKIRGYRIELREIETQLREYPEINQAIVVDQVYGNRKLLA
                     AYYVSDNKVSFGEIRKYLSDKLPEFMIPEKMIQVEEIPLNPNGKVDRKRLLDITQTDYV
                     SIPYVAPRNEIEQKLVRAWEKVMEIEGIGIHDNFFALKGESIKALQVINLLRKDCLKIS
                     TTDFFKHPTIAQLSSCVKLEHKEENFESKPTHVKDLSNPFSLTEVQTAYMLGRNSQFEL
                     SGISPQTYFEYETALDINRLSKSFQKVIHRHPMLRAVILPEGKQQILQSVPDYEIEIVS
                     LIDLDDRNQHARLQEERSRMTNHVFPLGQWPLFELKAFLLKEDTYLLCFRYDALLMDGA
                     SMNIVGQDLLHYYYKPNQKLESLSFDFQDYMFIYDEMEQSEEYKTAKDYWTSKLPDFPF
                     APSLLLKKDPAEIATPKFQSLTKILDNKKWTKLRKLAQEKEVTPSALLCTIYGDVLAYW
                     SNQRRLAINLTVFNRYPVHDEVEQIVGDFTSLILLDVDVKPEQTFFTRVKETQATLLDG
                     LEHRHYDGVNFIRDFTRYHQMTPKAVMPIVFTSMLAGAGAFSWEQLGSLRYIHARTPQV
                     YLDNVVIEKNGELLISWNYVEELFDVDVIEAMFSQFVDLLEQLVKQSDITSLQMKESDQ
                     TLIKQYNETTEKIPSTTLYQLFTDQVKRTPDEVAVVFEQEWLTYSELHKRSNQIAHFLK
                     EQGIGLGDKVGLLAKRRVDTIVNMLGILKSGAAYVPIDPDHPLDRQTYILKNSSCKLLL
                     EPSLYEENHLSFYTTEDMPAIAGPEDIAYIIYTSGSTGKPKGVIITHQAVTNTIQDINQ
                     KYEVNEDDRIIGISSMCFDLSVYDIFGALSTGAMLVMIRDPRDMQELIRTVERRGITIW
                     NTVPAIMDLALEQVGSHFEYPSLRLVLHSGDWIPLSLPEKIKRHFPIAEVVSLGGATEA
                     SIWSIYYPVKQVESHWNSIPYGMPLANQTYYVLNYEKKMCPVGVIGDLYIGGVGLAKGY
                     VNDEKKTNEAFVSHPDFGLIYKTGDCGKMHSEGYIEFLGRQDYQVKIQGYRVELEEISH
                     CLLTYKQVEHAVVIDQTDENGIKFLVAYVVTEQNISTTELRKHLRDHLPDYMIPSYFVY
                     LDQLPLTPNGKLDRKALPTPEKQKNKVFIAPKTEMEKILTSIWQEVLKIDQVGVNDHFF
                     ALGGDSIKAIQVSVRLYRQGFELEPKNIFSFPILRDMVQFVKKLDQFSSSQTNDVNIMN
                     DNKATLLNVKDKQLNQHTLNKIQEKMPDIKIERIYPLAPFQEVIYEHATNEPDTNAYFE
                     QTIWALEGELDIKLFIQCFQQLVDRHDSLRTIIVQDEQAKPWQAVLYDVQINSEIKNLI
                     EMTKQEQQEFLKQWMNENLSRGFKNDELMTRLCIFQLGNNEHKVIWSSHHLLCDGWSVS
                     ILIDELFQLYETTNAGTTAGLPMVKPFETFIDWTLAQDHEKARHFWRDYLSGYNQKISI
                     PKKKVPSKSEGLNFAFMDLTLDKDLTDQLQTFVTKNNITMNTALLAVWGILLGKFNGTR
                     EVVLPNLVSVRPPEVEGIENMFGLFTNVLPIRLAWTETQSFVDFLQKVQLETLKCNEYA
                     YYSFVDIQKQSELKTELTDHLWVFENYPTNPAIFSSNENRHFAITDYEVVDEPHVKYGI
                     MCFPEERLTIKFGYDQTVYETEKIQEILNHIQGLINTILQNPIQTICDYKL"
     primer_bind     complement(13724..13762)
                     
     primer_bind     13730..13783
                     
     CDS             13757..15211
                     /codon_start=1
                     /label=aminotransferase/decarboxylase
                     /translation="MTKKLQLSAEEMRQLGYQAVDLIVDHMNHLKSKPVSETIDSNIFR
                     DKLIETIPENGSNPKELLHFLNNNVFNQITHVDHPHFMAFVPGPNNYVGVLADFLASGF
                     NVFPTAWIVGAGAEQIELTTINWLKSMLGFPDSAEGLFVSGGSMANLTALTVARQVKLN
                     NDIENAIVYFSNQTHFSVDRALKVLGFKQHQICRIETDEDLKISVSTLRKQIKEDRLKG
                     KKPFCVIANAGTTNCGAVDSLDELADVCGDEDIWLHVDGAYGAAAILSEKGRELLSGIH
                     RADSLTLDPHKWLFQPYDVGCVLIRNSQYLSETFRMIPEYIKDTETNIEEKVNFGERGI
                     ELSRRFRALKVWLSFKAFGVTAFREAIDHGIMLAEQVEEFLRKEKDWEVVTPAQLGIVT
                     FRYIPCELTSTDTIHEINKKLVEEINQRGFAMLSTTKLKEKVVIRLCSINPRTTKEEIL
                     QIMMNIKALAEEINTSPKHLISVSQP"
     primer_bind     complement(15201..15252)
                     
     misc_feature    15230..15252
                     /label=HA
     primer_bind     15230..15249
                     /label=Primer 1
     CDS             15231..15956
                     /codon_start=1
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYKGS"
     terminator      15980..16027
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc aattcgttca
     4981 agccgaataa cttcgtataa tgtatgctat acgaacggta ttaagaccca ctttcacatt
     5041 taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
     5101 caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
     5161 taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
     5221 aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
     5281 ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
     5341 ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
     5401 tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
     5461 tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
     5521 acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
     5581 ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
     5641 acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
     5701 cctatcagtg atagagaaga ggatcgacca tgagcgagaa aatttattac tggtctccga
     5761 ttaagcattg ggagaagttg cataatgaag ttttgatagg ggaaatgaga tttacaggta
     5821 ttctgtctga gtggtttcca gatttttatt ttatggctca aaaaggtgtg aaaattagtg
     5881 aactagtaga gcgcttttcg ttaggaaatg tagaagagac acaaaaaaac atagagctca
     5941 tgataaaaaa tcgtgtgtta gtaagtaata tattacaccc aagagaagta ttttcttcac
     6001 aagaaaagat tttttcaaat ccatatagca accagattcg tttttctaaa gaaaagttag
     6061 ataagtatat gagtaaacaa ttaaatcgca cgcacgttgc tgcacggtcg accgaaattc
     6121 aacttgaaac aacaaatgag ctacctacta taatcaagga acgtaggtct tgtcgtcaat
     6181 ttgatatgga aaaacatatt agtttttcgg aattttcccg attcttatca actctaaaac
     6241 aggttaggga agaaaaaata tactatcatt acgcaagtgc aggcgggctc tatcccattg
     6301 atatctttgt ttatgtaaaa ccaaaacgta tagaggatat gaaagctgga ttttattatt
     6361 ataacccatc aaaaaatagt cttgtgattg taaacaacat tgatcaagta atcaaaagtg
     6421 atcatgagtt aattaatcag gatttattta ctcaatctgc cttctcagtt tatttggttt
     6481 ataatgcgaa cgcttccatc ccgaaatacg gttcagatgg atatttattt gcttgtattg
     6541 agtcaggcat tatcactgct accttaaata tggttgctga gacattaaat ttaggggttt
     6601 gttcagtcgg tcatatgaaa tttgaagata ttcagcaatt tttatgtttg gataatcatc
     6661 aagtgtttct tcatgggctt gaagttggct tgaaaattaa cgagtagaag aaatgaatat
     6721 tagggggata aagataaaaa tgcacattaa agatcgaagt tcgattcatg aaaataaaga
     6781 agtagaagcg ttttggaagg agcaattatc ttccgagatt ctcgattttt ctgtatttaa
     6841 atttaataac gattaccaac taaataaaga aacaccttgt gaatacgttc aagtcatagt
     6901 gaatgtagaa tttagtaaaa gtatacaaaa aataagtaag tctcaagatt tattattatt
     6961 tagctggttt caagccgctt taagggtttg tttggcacat tatacagatc aagaaagaat
     7021 tactattggt attcctataa ttgaaaagaa tcttgaacaa agtgagaatc ttaataaatt
     7081 gattccttta ggaagtgaaa tccagccgca tcacaccttt aaacaattag taaatgagat
     7141 tgaacaaacg actttattcg ggtatgaaaa tcaggagtat ccgctctctg aattattagt
     7201 taaacacaat ttaactcctt ttcaaattgt aatcgggatg gaaggattac ataatgaaaa
     7261 ttcgctagaa gaatacgcag agaataattt agccctatgg atacgaagaa aatggaatga
     7321 aatacataat gaatttgaat ttcaaatttc ttgcaagtcc cgttcattct cttctttgca
     7381 acagtttgcc aattcgtatt gttatgtttt aaagcaagtt tcacaaaatc cgaatttggc
     7441 attgaaagat atttgtatta ttgcagaaga agaaaaagag ttgttagaag gattgaatac
     7501 ttttcaaacg ggcatagact tgtttcaatc atttcaagat ttgtttgaag cgcaagtatt
     7561 aaaaactcca gatcaaattg ctgttgtttg taatgatcaa tctttgacat atagagaatt
     7621 gaataagaaa gcgaatcaat tggcttctat tcttcaaagt aaaggtgttt caaaagaaag
     7681 tattattggc gttatggtag atcggtcatt agaaatgatt attggtatga tggggatttt
     7741 aaaagcagga gcagcgtatt taccaatcga ccctaactac ccaactgaac gaattgaata
     7801 tatgttacaa gatagtcaag caaaatattt acttagtaag cgaacagagg aagtattgcc
     7861 gcaatttgcg ggtgaagtac tgtatcttga taaagaatat cttttccaag gtgaagaaag
     7921 taatttagta cgagaacata atcctaatga tttggcttat gttatttaca catcaggatc
     7981 gacaggaaat ccaaaagggg taatgataga acaaaaatcg ctagttcatt ttttaaatgt
     8041 aatgagagaa aaagtaaaag ctaatcaatc attcttgttt cttgctagtg tatcttttga
     8101 tatttccttg ttagaaatat gtcttccttt aacgcaaggt tcaaaagtag taattgctac
     8161 agaggaacag tttgcaacaa aagaattggc tcatttggtc aaaaaacata aggtggattt
     8221 atgggaatca acgccatcgc gaatggaagt gattttgagt gatccagaag gggcaaattt
     8281 tctaaaagat ttaaaatcta ttttattagc aggagaagca ttttccattg atttggttga
     8341 aaaaattcgt tgtatcagtg aagcaacaat tttaaatatt tatggtccca cagaaacaac
     8401 catttgcgca acagtaaaag acttgagcac ttccaaagaa gttacgatcg gcaaaccaaa
     8461 tccgaattat cattcctata tattgaataa gtatggccaa ttaaaaccat ttggaattcc
     8521 cggtgaactc tgcattgctg gagtagcggt agcacgaggg tatcttggta aaagtaaact
     8581 gacagatgaa aaatttgtac agagtccttt tgcggcaggg gaaatgatgt atcatacagg
     8641 tgatctcgtt cgttggttgc ctaatggaga acttgagtat ttaggtcgaa tggatcatca
     8701 agtgaaaatt cgagggtatc ggattgaatt aagagaaatc gaaactcaat taagggaata
     8761 tccagaaata aaccaggcta tcgttgttga tcaagtgtat ggaaatagga aattattagc
     8821 tgcctactat gtatcggata acaaggtgtc ttttggtgaa atcagaaaat atttaagtga
     8881 taaattacct gagtttatga ttcctgaaaa aatgattcag gtagaagaga ttccattgaa
     8941 tccaaatgga aaagtggata gaaaaaggtt attggatata acccaaacgg attatgtatc
     9001 aattccatac gttgccccgc gcaatgaaat tgaacaaaaa ttggtgcgtg cgtgggaaaa
     9061 agttatggag attgagggaa tcggtattca tgataatttt tttgcattaa aaggagaatc
     9121 cattaaagca cttcaggtaa ttaatttatt aagaaaagac tgcttaaaaa taagtacaac
     9181 agattttttt aagcacccta caatcgctca actgagttct tgtgtaaagt tagagcataa
     9241 agaagaaaac tttgaaagta aaccaactca tgttaaagat ttgtctaatc cattctcctt
     9301 aacagaagtt cagacagcgt atatgttagg aaggaattca cagtttgaat taagtggtat
     9361 ttcccctcaa acatattttg aatatgaaac agcattggat atcaatcgtc tttcaaaaag
     9421 ttttcaaaag gtgatccatc gtcatccaat gttgcgagct gtaattttac cagagggaaa
     9481 acagcaaata ttacaaagcg ttcctgatta cgagattgaa atagtgagtc tcatagattt
     9541 agatgatagg aatcaacatg ctcgcttaca agaagagcgt tctcgaatga ctaaccatgt
     9601 attccctttg gggcaatggc ctttgtttga attgaaagct tttcttttaa aagaagacac
     9661 gtatttatta tgttttcgat acgatgcttt attaatggac ggagcaagca tgaacattgt
     9721 tgggcaggat ttgttgcatt attattataa accaaatcaa aaattagaat ctttatcttt
     9781 tgattttcaa gattatatgt ttatctatga tgaaatggaa caaagtgaag aatataaaac
     9841 agcaaaagat tattggacaa gtaagcttcc tgactttccg tttgcacctt ctttgctgtt
     9901 aaaaaaggat ccagcagaga ttgctactcc taaattccaa tcacttacaa aaatcctgga
     9961 taataaaaaa tggacaaagc tacgaaagtt agcacaagag aaagaggtaa cgccttccgc
    10021 tctactttgt acaatatatg gtgatgtgtt ggcatattgg agtaatcaac gtcgattagc
    10081 aattaacttg actgtattta accgttatcc tgttcatgat gaagtagaac agattgttgg
    10141 agatttcaca tcacttattt tattggatgt tgatgtaaaa ccagagcaaa ctttctttac
    10201 tagagtaaaa gaaacgcaag ctactttatt agatggcctt gaacatcgtc attacgatgg
    10261 agttaacttt attcgagatt ttactcgata tcatcaaatg acgccaaaag cagttatgcc
    10321 aatcgttttc acctcgatgt tagcaggtgc aggtgctttc tcttgggaac aacttggttc
    10381 tcttcgctat attcatgctc ggactccaca agtgtatttg gataatgtag taattgaaaa
    10441 aaatggagag ttattaatta gctggaacta tgttgaagag ttgtttgatg ttgatgtaat
    10501 agaagcaatg ttttcccaat ttgttgactt attggaacaa ttggtgaaac aaagcgatat
    10561 aacatctttg cagatgaaag aatcggatca aactttaatt aagcaataca atgaaacaac
    10621 agaaaaaata ccttcaacta ctctgtatca attatttaca gaccaagtaa aacgtacacc
    10681 ggatgaagta gcagtggtat ttgaacaaga atggttaaca tactcggaac ttcataaacg
    10741 ttctaatcaa attgcccact tcttaaaaga gcaaggtatt ggtctaggtg acaaagtggg
    10801 tcttttagca aaaaggcgag ttgacacaat tgttaatatg ttaggtattt taaaatcagg
    10861 tgccgcttat gttccgattg atcccgatca tcctttagat cgccaaacat atattttaaa
    10921 aaatagctca tgcaagcttt tattagaacc tagtctttat gaagaaaatc atttgtcatt
    10981 ttatacaaca gaagatatgc ccgctattgc tggtccagaa gatattgcct atattatata
    11041 cacttcagga agtacaggaa aaccaaaagg agtaattatt actcaccaag cggttacaaa
    11101 tactattcaa gacataaatc aaaaatatga ggtgaatgaa gatgatcgta ttattggtat
    11161 ctcttccatg tgttttgacc tatctgtata cgatattttc ggagctttaa gcacaggtgc
    11221 aatgttagta atgattcgtg atccgagaga catgcaagaa ttaatccgca ctgtcgaacg
    11281 tagagggatt acaatttgga atacagtgcc tgcaatcatg gatttggcat tggagcaagt
    11341 aggttctcat tttgaatatc cttctttacg tttagtttta catagtggag attggattcc
    11401 gctttcttta ccagaaaaaa taaaacgaca ttttccgatt gcagaagtag tctcactagg
    11461 aggggcaaca gaagcatcga tttggtcaat ttattatccg gtcaaacagg tggaatcaca
    11521 ttggaatagt attccatacg ggatgccctt ggcaaaccaa acgtattatg tgttgaacta
    11581 tgaaaagaaa atgtgccctg ttggtgtaat tggagattta tatatcgggg gagtgggcct
    11641 tgctaaagga tatgtaaatg atgaaaagaa aacaaatgaa gcgtttgtgt cacatcctga
    11701 ttttggtctt atatataaaa caggagattg tggaaaaatg cattctgagg gatatattga
    11761 atttttaggc cgccaagatt accaagtgaa aattcaggga tatcgagtag agttagaaga
    11821 aatctctcat tgtcttctaa cttataaaca agtcgaacat gctgtcgtaa ttgatcaaac
    11881 agatgagaac ggaataaaat ttttagttgc atatgtggta actgagcaaa atattagtac
    11941 aacagaacta cgaaagcatt tgcgagatca cttgccagat tatatgattc cttcttattt
    12001 tgtttatttg gaccaattac ccctgactcc aaacgggaaa ttagatagaa aagcgctccc
    12061 tactccggag aaacaaaaaa ataaagtatt tattgcccca aaaactgaaa tggaaaaaat
    12121 attaactagt atttggcaag aagtactcaa aatagatcaa gttggagtaa atgatcattt
    12181 cttcgcctta ggtggagatt cgataaaagc gattcaagtg tccgtcagac tgtatcgaca
    12241 aggattcgag cttgaaccaa aaaatatatt ttcatttcct atattaaggg acatggttca
    12301 atttgttaaa aagctagacc agttttcaag ttcacaaaca aatgatgtaa acataatgaa
    12361 cgataataaa gctacattac taaatgttaa agataaacaa cttaatcaac atacacttaa
    12421 caaaatacaa gaaaaaatgc cggatataaa aatagaacgt atttacccgt tagcaccttt
    12481 ccaggaagtt atttatgagc atgctacgaa tgaacctgat acaaatgcat attttgaaca
    12541 aacaatttgg gcacttgagg gggagctgga tattaaactc tttattcagt gtttccaaca
    12601 attagttgat cgtcatgatt ctttaagaac aataattgtg caagatgaac aagcaaaacc
    12661 ttggcaagct gttctatatg atgttcaaat aaattcagaa ataaaaaact tgattgagat
    12721 gacgaaacaa gaacagcaag aattcctaaa acagtggatg aatgaaaatt taagtcgagg
    12781 atttaaaaat gatgaactta tgacacgtct ctgtatattc caactaggta ataacgagca
    12841 taaagtaatt tggagttccc atcatctatt atgtgatggt tggagcgtca gcattttgat
    12901 agacgaatta ttccagctct acgaaactac aaatgccgga actacggcgg ggttacccat
    12961 ggtaaaacct tttgaaacat ttatagattg gactttggca caagatcatg agaaagcacg
    13021 tcatttttgg cgagattatt tatcaggata caatcagaaa attagtattc ctaaaaagaa
    13081 agtcccatct aaaagtgaag ggttaaactt cgcttttatg gatttaacat tagacaagga
    13141 tttgactgat cagttacaga catttgtaac taaaaataat ataactatga atacggcatt
    13201 actagctgta tggggaatat tgcttggcaa attcaacgga acgagagaag tagtattgcc
    13261 taatctagta tcagtcagac ctcccgaagt agaaggaatt gaaaatatgt tcggtctgtt
    13321 cacaaatgtt ttgcctatcc gattagcatg gacagaaacg caatcttttg tggacttcct
    13381 gcaaaaggta caactagaga cattgaaatg taatgagtat gcatactatt catttgttga
    13441 tatacaaaag caaagtgaat taaaaactga gttgactgac catctttggg tttttgaaaa
    13501 ctatccaact aatccagcaa tcttttcttc aaatgaaaac agacattttg ctatcacgga
    13561 ttacgaagtt gttgatgagc cacatgtaaa gtatggaatt atgtgcttcc ctgaagaaag
    13621 acttacaata aagtttggat atgatcaaac ggtatatgaa acagagaaaa tacaggaaat
    13681 attaaatcac atccaggggt taatcaacac gattcttcaa aatccaatac aaacaatttg
    13741 tgattacaaa ttataaatga caaaaaaatt acagttatca gccgaagaaa tgcgtcagtt
    13801 aggatatcaa gcggttgact taattgttga tcatatgaat catttaaaaa gtaaaccagt
    13861 ttcagaaacg attgatagta atatttttag agacaagcta attgaaacga ttccggaaaa
    13921 cggatctaat ccaaaagaat tacttcattt tcttaataac aatgtgttta atcaaattac
    13981 tcatgtggat catcctcatt ttatggcttt tgtgccaggt cctaataatt atgttggtgt
    14041 attagcagat tttttagcaa gtgggtttaa tgtatttcct acagcttgga tagtaggtgc
    14101 aggtgccgaa caaattgaat taacgacaat taactggtta aaatcaatgc taggattccc
    14161 tgattcagct gaaggattat ttgtcagtgg tggttccatg gctaatttga ctgcacttac
    14221 tgtagcgaga caggtcaagc tgaataatga catagaaaat gcgattgttt atttttctaa
    14281 ccaaactcat ttttctgtag atcgagcact taaagtatta ggttttaaac agcatcaaat
    14341 ttgccgaatt gagacagatg aagacttaaa aatttcagtt agtactttaa gaaaacaaat
    14401 aaaagaagat cgattaaaag ggaaaaaacc attctgtgtt attgctaatg cagggacaac
    14461 gaattgcgga gcggtggact ccctcgatga attggctgat gtatgtggtg atgaagatat
    14521 atggctacac gtggatgggg cttatggggc tgcagcaatt ctcagtgaaa aaggaaggga
    14581 gttattaagt ggaattcatc gtgccgattc tttaacgtta gacccgcata aatggctttt
    14641 tcagccttat gatgtcggtt gtgtacttat tcgaaatagc caatatttaa gtgaaacatt
    14701 ccgtatgatc ccagagtata ttaaggatac agaaactaat atagaagaaa aagtgaattt
    14761 tggggagcgt ggaattgaac tttctcgtag atttagagca ttaaaggtat ggctttcttt
    14821 taaagcattc ggagttacag cttttcgtga ggcaattgat catggcatta tgctagctga
    14881 acaagttgaa gagtttttaa ggaaagaaaa agattgggaa gtagtaacac ctgcccaatt
    14941 aggaattgtt acttttcgtt atattccatg tgagctaaca tctacagata caattcatga
    15001 aataaacaaa aaattggtgg aagaaattaa tcaaagagga tttgctatgc taagtacgac
    15061 taaattaaaa gaaaaagtag taattcgact ttgctctatc aacccaagaa caacaaaaga
    15121 agaaatactt caaatcatga tgaacataaa agcattagca gaggaaataa acacatcgcc
    15181 taaacatctt atatctgttt ctcaaccttg atatattagg cggaggcctc atggttagca
    15241 aaggtgaaga actgtttacc ggcgttgtgc cgattctggt ggaactggat ggtgatgtga
    15301 atggccataa atttagcgtt cgtggcgaag gcgaaggtga tgcgaccaac ggtaaactga
    15361 ccctgaaatt tatttgcacc accggtaaac tgccggttcc gtggccgacc ctggtgacca
    15421 ccctgaccta tggcgttcag tgctttagcc gctatccgga tcatatgaaa cgccatgatt
    15481 tctttaaaag cgcgatgccg gaaggctatg tgcaggaacg taccattagc ttcaaagatg
    15541 atggcaccta taaaacccgt gcggaagtta aatttgaagg cgataccctg gtgaaccgca
    15601 ttgaactgaa aggtattgat tttaaagaag atggcaacat tctgggtcat aaactggaat
    15661 ataatttcaa cagccataat gtgtatatta ccgccgataa acagaaaaat ggcatcaaag
    15721 cgaactttaa aatccgtcac aacgtggaag atggtagcgt gcagctggcg gatcattatc
    15781 agcagaatac cccgattggt gatggcccgg tgctgctgcc ggataatcat tatctgagca
    15841 cccagagcgt tctgagcaaa gatccgaatg aaaaacgtga tcatatggtg ctgctggaat
    15901 ttgttaccgc cgcgggcatt acccacggta tggatgaact gtataaaggc agctaaggca
    15961 gagtggtaaa atgcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
    16021 ttttttgctg aaaggaggaa ctatatccgg at
//
