LOCUS       Exported                7073 bp DNA     circular SYN 04-MAY-2022
DEFINITION  martin aichinger zuber lab.
ACCESSION   urn.local...10-33pqa34
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 4, 2022 from SnapGene 6.0.4
            https://www.snapgene.com
COMMENT     p76 + g loxP STOP
FEATURES             Location/Qualifiers
     source          1..7073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1977..2032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1978..1988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1978..1988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..684
                     /label=5'LTR
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             514..689
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    555
                     /label=T
                     /label=nonstandard type: Editing History Replacement
                     /note="/Original_Bases=T"
     misc_feature    657..678
                     /label=337 (f)
     misc_feature    690..1568
                     /label=Psi+
                     /label=nonstandard type: misc
                     /note="/%_Identity=100"
                     /note="/Mismatches=0"
                     /note="/Motif=TGGGGGCTCGTCCGGGATTTGGAGACCCCTGCCCAGGGACCACCG
                      ACCCA 
                     CCACCGGGAGGTAAGCTGGCCAGCAACTTATCTGTGTCTGTCCGATTGTCTAGTGTCT 
                     ATGTTTGATGTTATGCGCCTGCGTCTGTACTAGTTAGCTAACTAGCTCTGTATCTGGC 
                     GGACCCGTGGTGGAACTGACGAGTTCTGAACACCCGGCCGCAACCCTGGGAGACGTCC 
                     CAGGGACTTTGGGGGCCGTTTTTGTGGCCCGACCTGAGGAAGGGAGTCGATGTGGAAT 
                     CCGACCCCGTCAGGATATGTGGTTCTGGTAGGAGACGAGAACCTAAAACAGTTCCCGC 
                     CTCCGTCTGAATTTTTGCTTTCGGTTTGGAACCGAAGCCGCGCGTCTTGTCTGCTGCA 
                     GCGCTGCAGCATCGTTCTGTGTTGTCTCTGTCTGACTGTGTTTCTGTATTTGTCTGAA 
                     AATTAGGGCCAGACTGTTACCACTCCCTTAAGTTTGACCTTAGGTCACTGGAAAGATG 
                     TCGAGCGGATCGCTCACAACCAGTCGGTAGATGTCAAGAAGAGACGTTGGGTTACCTT 
                     CTGCTCTGCAGAATGGCCAACCTTTAACGTCGGATGGCCGCGAGACGGCACCTTTAAC 
                     CGAGACCTCATCACCCAGGTTAAGATCAAGGTCTTTTCACCTGGCCCGCATGGACACC 
                     CAGACCAGGTCCCCTACATCGTGACCTGGGAAGCCTTGGCTTTTGACCCCCCTCCCTG 
                     GGTCAAGCCCTTTGTACACCCTAAGCCTCCGCCTCCTCTTCCTCCATCCGCCCCGTCT 
                     CTCCCCCTTGAACCTCCTCGTTCGACCCCGCCTCGATCCTCCCTTTATCCAGCCCTCA 
                     CTCCTTCTCTAGGCGCC"
                     /note="/Transferred_From=Psi+"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/annotation_group=Psi+: 1 -> 879"
     misc_feature    752..951
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1152..1568
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1572..1583
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1666..1869
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_recomb     1870..1903
                     /label=LoxP
     misc_feature    1870..1903
                     /label=loxP
     protein_bind    1870..1903
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1904..1942
                     /label=STOP+MCS
     misc_recomb     1943..1976
                     /label=LoxP
                     /label=LoxP(1)
     misc_feature    1943..1976
                     /label=loxP
                     /label=loxP(1)
     protein_bind    1943..1976
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    join(1990..1995,1997..2002,2004..2009)
                     /label=BpiI Block
     misc_feature    2283..2791
                     /label=mouse pgk
                     /label=nonstandard type: promotor
                     /note="/%_Identity=100"
                     /note="/Mismatches=0"
                     /note="/Motif=AATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAG
                      CATGC 
                     GCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACAC 
                     ATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCC 
                     ACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGC 
                     AGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCG 
                     CTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTT 
                     CGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGG 
                     CTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTC 
                     AAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCG"
                     /note="/Transferred_From=mouse pgk"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/annotation_group=mouse pgk: 1 -> 509"
     promoter        2292..2791
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2805..3608
                     /label=Neo
                     /note="/%_Identity=100"
                     /note="/Mismatches=0"
                     /note="/Motif=ATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCG
                      GCCGC 
                     TTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGAT 
                     GCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACC 
                     TGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCAC 
                     GACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGG 
                     CTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCG 
                     AGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTAC 
                     CTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAA 
                     GCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCG 
                     AACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCA 
                     TGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATC 
                     GACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTG 
                     ATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTAT 
                     CGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA 
                     "
                     /note="/Transferred_From=Neo"
                     /note="/Transferred_Similarity=97.54%"
                     /note="/annotation_group=Neo: 1 -> 804"
     LTR             3726..4346
                     /label=LTR-SIN
     LTR             3779..4204
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     misc_feature    3960..3980
                     /label=1160 R
     misc_feature    4424..4444
                     /label=339 (r)
     promoter        4470..4799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4650..4785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(5092..5680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5851..6711)
                     /label=AMP
     promoter        complement(6712..6816)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    complement(6753..6781)
                     /direction=LEFT
                     /label=AMP promoter
                     /label=nonstandard type: promotor
                     /note="/%_Identity=100"
                     /note="/Motif=ATTGTCTCATGAGCGGATACATATTTGAA"
                     /note="/Transferred_From=amp promotor"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/annotation_group=AMP promoter: 1 -> 29"
                     /note="/modified_by=User"
     misc_feature    6913
                     /label=T
                     /label=T(1)
                     /label=nonstandard type: Editing History Replacement
                     /note="/Original_Bases=T"
     misc_feature    6993..7020
                     /label=340 (f)
     enhancer        join(7002..7073,1..309)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtctcccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1561 taggcgccgg aattgaaggt cgtagatctc cgcggttaat taaaatgata cggcgaccac
     1621 cgagatctgt cttgatctag gagctactca tcaacggtca catgtgaggg cctatttccc
     1681 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     1741 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1801 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1861 tgaaagtata taacttcgta tactttatat tatacgaagt tatttttttt ttttgcggcc
     1921 gcccagaatt ctggcctgca ggataacttc gtatacttta tattatacga agttatgacg
     1981 aaacaccggg tcttcgctcg agagaagacc tgtttaagag ctatgctgga aacagcatag
     2041 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     2101 tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg
     2161 ccaattctgc agacaaatgg ctctagaggt acccgttaag atcggaagag cacacgtctg
     2221 aactccagtc acaaaattat ctcgtatgcc gtcttatgct tgttaattaa caattgacgc
     2281 gtaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2341 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2401 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2461 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2521 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2581 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     2641 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     2701 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     2761 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
     2821 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2881 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2941 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     3001 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3061 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3121 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3181 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3241 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3301 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3361 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3421 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     3481 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     3541 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3601 tcttctgagg ggatccgtcg acctgcagcc aagcttatcg ataccgtcga gatttaatta
     3661 agatatcatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     3721 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     3781 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     3841 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     3901 ggtcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     3961 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     4021 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     4081 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     4141 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     4201 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
     4261 ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4321 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4381 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     4441 atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4501 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4621 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4801 agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     4861 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     4921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5521 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     6181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     6481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6841 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     6901 gccctttcgt ctacaagaat tagcttggcc attgcatacg ttgtatccat atcataatat
     6961 gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat tattgactag
     7021 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agt
//
