LOCUS       lentiCas9_U6_IT_       13294 bp ds-DNA     circular     15-JAN-2016
DEFINITION  lentiCas9-Blast.
ACCESSION   urn.local...oj-5hx36zc
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors. .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        239..815
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro, lentiCRISPR
                     v2 (modified), lentiCas9-Hygro, pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=CMV_immearly_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     2886..2919
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    772..792
                     /label=CMV_fwd_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     7878..7911
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     ltr             781..1016
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="87.71%"
                     /label=3' LTR SIN
                     /ApEinfo_fwdcolor=#8882be
                     /ApEinfo_revcolor=#8882be
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2920..2930
                     /label=STOP+MCS
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=#808080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1017..1034
                     /gene="PBS"
                     /SECDrawAs="Region"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1;
                     pRRL.EF1alpha.CPPT.rtTA3.IRES.Luc2noSTOP.P2A.TagBFP.WPRE -
                     PBS; pRRL.EF1alpha.rtTA3.IRES.Luc2no stop.P2A.TagBFP.
                     (RRLM-RIL2BFP no stop) - PBS; pRRL.LinkM (RRLM) - PBS;
                     pRRL.Linker (2 AgeI sites) - PBS;
                     pRRL.PPT.SF.EGFP.IRES.Puro.PTEN213158.WPRE_29-05-2012 -
                     PBS; pRRL.PPT.SF.EGFP.LMO2-miR30.WPRE_Maxi_29-05-2012 -
                     PBS; pRRL.PPT.SF.GFP.miR30.WPRE (Schambach) - PBS;
                     pRRL.PPT.SF.rtTA3-IRES-Luc2-P2A-TagBFP - PBS;
                     pRRL.Recipient (1 AgeI site) - PBS;
                     pRRL.SFFV.rtTA3.IRES.Luc2no stop.P2A.TagBFP. (RRLM-RIL2BFP
                     no stop) - PBS; pRRL.TRE.dsRED.miR30.PGK.Venus.IRES.rtTA3
                     (lenti-TRMPVIR) - PBS; pRRL.TRE.mCherry.miR30.PGK.Venus
                     (lenti-TCMPV) - PBS;
                     pRRL.TRE3G.GFP.miRE.PGK.Puro.IRES.rtTA3 (lenti-T3GGmePPIR)
                     - PBS; pRRL.TRE3G.dsRED.miRE.PGK.Venus.IRES.rtTA3
                     (lenti-T3GRmePVIR) - PBS; pRRL.TRE3G.empty - PBS;
                     pRRL.cPPT.EF1alpha.rtTA3.IRES.Luc2noSTOP.P2A.TagBFP.WPRE -
                     PBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, lentiCRISPR v2 (modified), lentiGuide-Puro
                     (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=PBS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2886..2919
                     /label=loxP
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1126..1170
                     /SECDrawAs="Label"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=psi
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7878..7911
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#008040
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1233..1252
                     /Sequence="GTACAGTGCAGGGGAAAGAA"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1585..1600
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLV-tTRKRAB
                     (modified)"
                     /X-Transferred_Similarity="100.00%; 68.75%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=SV40_int
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1674..1972
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pRSI9-U6-sh-UbiC-TagRFP-2A-Puro (DECIPHER) - RRE"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=RRE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2132..2151
                     /Sequence="GGGCAAGTTTGTGGAATTGG"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; lentiCrispr mCherryP2AneoR-improved tracer;
                     pLentigRNASQF; pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, lentiCRISPR v2 (modified), lentiCrispr
                     mCherryP2AneoR-improved tracer, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak - improved backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(1)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2174..2187
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW*"
                     /Transferred_From="pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="81.25%"
                     /label=SV40_int(1)
                     /ApEinfo_label=SV40_int
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2417..2439
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1; plKO1 3xLac - HRP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, lentiCRISPR v2 (modified), lentiGuide-Puro
                     (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=HRP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2437..2560
                     /gene="PPT"
                     /SECDrawAs="Region"
                     /X-Transferred_From="RIEPTETronly; RIKPB; RIKPH; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP);
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP) Copy"
                     /X-Transferred_Similarity="100.00%; 95.97%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=PPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2440..2453
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2440..2557
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiCas9-Hygro; lentiCrispr
                     mCherryP2AneoR; lentiCrispr mCherryP2AneoR-improved
                     tracer; pHR-SFFV-dCas9-BFP-KRAB (modified); pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improved tracer, backup of
                     lentiCas9-Hygro, lentiCRISPR v2 (modified),
                     lentiCas9-Blast (modified), lentiCas9-Hygro, lentiCrispr
                     mCherryP2AneoR, lentiCrispr mCherryP2AneoR-improved
                     tracer, pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB
                     (modified), pLentiCRISPR, pLentiCRISPR-P2A-Thy1.1,
                     pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1, pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI
                     Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT(1)
                     /ApEinfo_label=cPPT
                     /ApEinfo_fwdcolor=#fdc68a
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2441..2564
                     /Description="central polypurine tract (cPPT) and the
                     central termination sequence (CTS)"
                     /X-Transferred_From="FUW-OSKM - cPPT/CTS;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified);
                     pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE
                     (L3beta XbaI linker + SpeI linker) Mareike - cPPT/CTS;
                     pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE
                     (L3beta XbaI linker) Mareike - cPPT/CTS; pLKO.3G_Mareike -
                     cPPT/CTS; pLentiCRISPR-P2A-Thy1.1; pSIN-U6-PGK-Venus
                     (SUPV) - cPPT; pSIN-U6-TuD-anti miR-34a-PGK-Venus
                     (SUPV-miR-34a) - cPPT; pSIN-U6-TuD-anti miR150-PGK-Venus
                     (SUPV-miR150) - cPPT; pSIN-U6-TuD-anti miR21a-PGK-Venus
                     (SUPV-miR21a) - cPPT; pSIN-U6-TuD-anti miR29a-PGK-Venus
                     (SUPV-miR29a) - cPPT; pSIN-U6-TuD-anti shRen-PGK-Venus
                     (SUPV-shRen) - cPPT; pSIN-U6-TuD-anti shRen713-PGK-Venus
                     (SUPV-Ren) - cPPT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, backup of lentiCas9-Hygro, lentiCRISPR v2
                     (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pCRISPR-P2A-puro,
                     pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT; cPPT/CTS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2441..2558
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pWPXLd - CPPT/CTS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, backup of lentiCas9-Hygro, lentiCRISPR v2
                     (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=CPPT/CTS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2444..2465
                     /Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGN
                     VAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALT
                     IPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAF
                     ALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTVDLET*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCRISPR
                     13450; lentiCRISPR v2 (modified); lentiCrispr
                     mCherryP2AneoR; lentiCrispr mCherryP2AneoR-improved
                     tracer; pCW-Cas9 (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improved tracer, lentiCRISPR
                     13450, lentiCas9-Blast (modified), lentiCrispr
                     mCherryP2AneoR, lentiCrispr mCherryP2AneoR-improved
                     tracer, pCW-Cas9 (modified), pHR-SFFV-dCas9-BFP-KRAB
                     (modified), pLentiCRISPR-P2A-Thy1.1, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="86.36%"
                     /label=U3PPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2444..2459
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
                     /label=cPPT(2)
                     /ApEinfo_label=cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2467..2486
                     /Sequence="GTACAGTGCAGGGGAAAGAA"
                     /X-Transferred_From="185_primer_bind12;
                     _185_primer_bind12; backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; primer_bind12"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, lentiGuide-Puro (modified),
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(2)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2479..2522)
                     /created_by="User"
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=R1 CRISPR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2479..2522)
                     /Sequence="CTTTAGTTTGTATGTCTGTTGCTATTATGTCTACTATTCTTTCC"
                     /X-Transferred_From="285_R1 Crispr; R1 Crispr; _285_R1
                     Crispr; backup of lentiCas9-Hygro; lentiCas9-Blast
                     (modified); lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentisgRNA Thy1.1-P2A-neo +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pCRISPR-P2A-puro,
                     pHR-SFFV-dCas9-BFP-KRAB (modified), pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(3)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2601..2840
                     /X-Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo;
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /label=hU6_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2841..2868
                     /X-Transferred_From="final_retro_CRIS"
                     /X-Transferred_Similarity="72.01%"
                     /modified_by="User"
                     /Transferred_From="pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: concatenated sequence"
                     /label=Filler
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 2869..2885
                     /created_by="User"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer, pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1, pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI
                     Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=improved tracer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7974..7983
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     lentiCrispr mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro, lentiCRISPR
                     v2 (modified), lentiCrispr mCherryP2AneoR,
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=U6 terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    7986..8574
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=WPRE
                     /ApEinfo_fwdcolor=#bc8dbf
                     /ApEinfo_revcolor=#bc8dbf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8758..8771
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT(3)
                     /ApEinfo_label=cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8762..8783
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=U3PPT(1)
                     /ApEinfo_label=U3PPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8762..8777
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=cPPT(4)
                     /ApEinfo_label=cPPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8778..8831
                     /invitrogen=1410000
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; lentiGuide-Puro (modified);
                     plenti6gw_u6laminshrna_verB - delta U3"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, lentiGuide-Puro (modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="81.48%"
                     /label=delta U3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8779..8831
                     /Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGN
                     VAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALT
                     IPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAF
                     ALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;
                     LentiCrisprmCherryP2Apuro-improved tracer; lentiCRISPR
                     13450; lentiCRISPR v2 (modified); lentiCrispr
                     mCherryP2AneoR; lentiCrispr mCherryP2AneoR-improved
                     tracer; lentiGuide-Puro (modified); pCW-Cas9 (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDLLFACTGSLWLDQI*;
                     MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFP
                     GLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAG
                     RPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRVDFKTNDLQGSC
                     RS*;
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPVSPRRPD
                     *"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improved tracer, lentiCRISPR
                     13450, lentiCrispr mCherryP2AneoR, lentiCrispr
                     mCherryP2AneoR-improved tracer, lentiGuide-Puro
                     (modified), pCW-Cas9 (modified), pHR-SFFV-dCas9-BFP-KRAB
                     (modified), pLentiCRISPR-P2A-Thy1.1, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="81.13%"
                     /label=delta_U3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     ltr             9045..9280
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="86.44%"
                     /label=3' LTR SIN(1)
                     /ApEinfo_label=3' LTR SIN
                     /ApEinfo_fwdcolor=#8882be
                     /ApEinfo_revcolor=#8882be
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     cds             9287..9518
                     /Transferred_From="PX335 D10N PV"
                     /Transferred_Similarity="97.41%"
                     /label=bGH polyA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(9305..9322)
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=BGH_rev_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(9500..9526)
                     /Sequence="GCGGCCGCTCCCCAGCATGCCTGCTAT"
                     /X-Transferred_From="NotI bGHR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="PX330"
                     /Transferred_Similarity="77.78%"
                     /label=Binding Region(4)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      9581..10036
                     /X-Transferred_From="pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiGuide-Puro (modified),
                     pLentiCRISPR, pLentisgRNA Thy1.1-P2A-neo +Kosak,
                     pLentisgRNA Thy1.1-P2A-neo +Kosak - improved backbone
                     andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="95.18%"
                     /label=F1 ori
                     /ApEinfo_fwdcolor=#f49ac2
                     /ApEinfo_revcolor=#f49ac2
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        10036..10304
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=SV40_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        10398..10465
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=EM7_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            10466..10837
                     /gene="bleo"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=bleo
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polya_signal    10970..11100
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; lentiGuide-Puro (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro, lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified),
                     lentiCas9-Hygro, lentiGuide-Puro (modified),
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentiCRISPR, pLentiCRISPR-P2A-Thy1.1, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak -
                     improved backbone andkilled BsmBi site in thy1.1,
                     pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=SV40 polyadenylation signal
                     /ApEinfo_fwdcolor=#6ecff6
                     /ApEinfo_revcolor=#6ecff6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11130..11359
                     /Description="lac operon sequence"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pGEM4z (Promega)@1 -
                     Misc_Feature_4; pGEM4z-Bcl2 (G145A)@1 - Misc_Feature_4;
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, backup of lentiCas9-Hygro, lentiCRISPR v2
                     (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pCRISPR-P2A-puro,
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=Misc_Feature_4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(11358..11372)
                     /Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKV
                     GIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
                     LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
                     DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGH
                     FLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENL
                     IAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIG
                     DQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
                     GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLY
                     EYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE
                     CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
                     IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFA
                     NRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDEL
                     VKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQ
                     LQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN
                     RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQL
                     VETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREIN
                     NYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYF
                     FYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIV
                     KKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGK
                     SKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRK
                     RMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF
                     DTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKKK*
                     /X-Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKR
                     KVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG
                     ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLV
                     EEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
                     GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALV
                     RQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNRED
                     LLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL
                     ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSL
                     LYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKK
                     IECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDR
                     EMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDG
                     FANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD
                     ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVEN
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSD
                     KNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKR
                     QLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE
                     INNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAK
                     YFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVN
                     IVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
                     RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFK
                     YFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKK
                     K*"
                     /X-Transferred_From="lentiCRISPR 13450; pCW-Cas9
                     (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR 13450, pCW-Cas9 (modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="80.00%"
                     /label=NLS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(11359..11422)
                     /Description=" L-strand Y effector site ; L-strand Y
                     effector site"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=L_Y_EFF
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(11364..11375)
                     /product="3' stem, pBR pas(L)"
                     /gene="pas'"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=(pas')
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(11407..11417)
                     /product="5' stem, pBR primosome ass'y site (L)"
                     /gene="pas"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=(pas)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11483..12156
                     /Description="derived originally from a ColE1-like
                     plasmid, pMB1"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; backup of lentiCas9-Hygro; lentiCRISPR v2
                     (modified); lentiCas9-Hygro; pIBHistoneH2B-mCherry - pUC
                     ori"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer, backup of lentiCas9-Hygro, lentiCRISPR v2
                     (modified), lentiCas9-Hygro, pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=pUC ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     repeat_unit     complement(12154..12191)
                     /Description=" Tn3 inverted repeat "
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=TN3_INV_RPT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     cds             12301..13161
                     /X-Transferred_From="Reporter Puro_P2A-tGFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="TPGPB-Cas9(PX330)"
                     /Transferred_Similarity="99.77%"
                     /label=Amp
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(13162..13260)
                     /invitrogen=2330000
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter uukaryotic"
                     /label=bla promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 7912..7982
                     /created_by="User"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer, pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1, pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI
                     Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=improved tracer(1)
                     /ApEinfo_label=improved tracer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7912..7983
                     /label=to add
                     /ApEinfo_fwdcolor=#37f807
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2953..3203
                     /X-Transferred_From="pLentiCRISPR;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improved tracer, pLentiCRISPR,
                     pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1"
                     /Transferred_Similarity="94.02%"
                     /note="Geneious type: promoter prokaryotic"
                     /label=EFIas; EFIas; EFS; EFS; EFS
                     /ApEinfo_fwdcolor=#bc8dbf
                     /ApEinfo_revcolor=#bc8dbf
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3217..7365
                     /Description="hSpCsn1"
                     /X-Transferred_From="PX260@5; PX330; PX330@5"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="PX260@5, PX330, PX330@5, lentiCRISPR v2
                     (modified), PX334, PX334@5, PX335, PX335@5"
                     /Transferred_Similarity="99.98% -> 99.93%"
                     /label=hSpCsn1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'clip          4359..4923
                     /X-Transferred_From="PX335@5"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="PX335@5, backup of lentiCas9-Hygro,
                     lentiCas9-Hygro"
                     /Transferred_Similarity="100.00%"
                     /label=SQ4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4545..4559)
                     /Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKV
                     GIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
                     LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
                     DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGH
                     FLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENL
                     IAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIG
                     DQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
                     GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLY
                     EYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE
                     CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
                     IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFA
                     NRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDEL
                     VKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQ
                     LQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN
                     RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQL
                     VETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREIN
                     NYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYF
                     FYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIV
                     KKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGK
                     SKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRK
                     RMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF
                     DTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKKK*
                     /X-Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKR
                     KVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIG
                     ALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLV
                     EEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
                     GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALV
                     RQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNRED
                     LLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPL
                     ARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSL
                     LYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKK
                     IECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDR
                     EMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDG
                     FANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD
                     ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVEN
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSD
                     KNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKR
                     QLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE
                     INNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAK
                     YFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVN
                     IVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEK
                     GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
                     RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFK
                     YFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKK
                     K*"
                     /X-Transferred_From="lentiCRISPR 13450; pCW-Cas9
                     (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR 13450, pCW-Cas9 (modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="80.00%"
                     /label=NLS(1)
                     /ApEinfo_label=NLS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7366..7389
                     /translation="MQIEKHLSAPSAGAQDAPVLISDRDDTSQVASCRSSSSAQHHEF
                     CTRSPSKMIYIDTSEDAAVARESCAGDAVVFRDGDAVDCSRCSFNEGGFFLRQRLGHR
                     SRILFDISGLFQQREVCCAGSLIVIVFVIFLLLSLSSLLCGGRSLVASQLRQVDPCLV
                     QAGDALVDQGGVQHLFGAGVPLPVDGGVKVLEGGRGSQIGQGKQVDDILGLLSDGLIP
                     VLVVGGQHFVQISVGQDHSLGELADLLDDLVQVVLVLFHKQLFLLIILGGALQLLIVA
                     GQVQEVHIFGGQGQFVSLLQFAGRGQHSLPAVFQLEQGVLRQLDDQVLFHFFVALGFQ
                     KVDGILLEAASFHDGDPQ*"
                     /label=FLAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7390..7455
                     /created_by="User"
                     /label=P2A
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            7456..7854
                     /gene="blast"
                     /translation="MQIEKHLSAPSAGAQDAPVLISDRDDTSQVASCRSSSSAQHHEF
                     CTRSPSKMIYIDTSEDAAVARESCAGDAVVFRDGDAVDCSRCSFNEGGFFLRQRLGHR
                     SRILFDISGLFQQREVCCAGSLIVIVFVIFLLLSLSSLLCGGRSLVASQLRQVDPCLV
                     QAGDALVDQGGVQHLFGAGVPLPVDGGVKVLEGGRGSQIGQGKQVDDILGLLSDGLIP
                     VLVVGGQHFVQISVGQDHSLGELADLLDDLVQVVLVLFHKQLFLLIILGGALQLLIVA
                     GQVQEVHIFGGQGQFVSLLQFAGRGQHSLPAVFQLEQGVLRQLDDQVLFHFFVALGFQ
                     KVDGILLEAASFHDGDPQ*"
                     /label=blast
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta tttcccatga ttccttcata tttgcatata cgatacaagg
     2641 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     2701 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     2761 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2821 tgtggaaagg acgaaacacc ggagacgatc agtatacgta acgtctctgt ttaagagcta
     2881 tgctgataac ttcgtatagc atacattata cgaagttatT TTTTTTTTTT tagctaggtc
     2941 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     3001 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
     3061 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     3121 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     3181 agaacacagg accggttcta gagcgctgcc accatggaca agaagtacag catcggcctg
     3241 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     3301 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     3361 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3421 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3481 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3541 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3601 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3661 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3721 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3781 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     3841 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     3901 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     3961 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     4021 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     4081 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     4141 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     4201 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     4261 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     4321 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4381 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4441 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4501 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4561 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4621 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4681 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4741 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4801 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     4861 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     4921 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     4981 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     5041 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     5101 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     5161 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     5221 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     5281 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     5341 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5401 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5461 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5521 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5581 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5641 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5701 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5761 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     5821 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     5881 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     5941 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     6001 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     6061 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     6121 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     6181 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     6241 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     6301 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6361 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     6421 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     6481 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6541 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6601 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6661 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tactggggat caccatcatg
     6721 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6781 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     6841 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     6901 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     6961 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     7021 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     7081 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     7141 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     7201 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     7261 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaag
     7321 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagatta caaagacgat
     7381 gacgataagg gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa
     7441 gagaatcctg gaccgatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     7501 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     7561 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     7621 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     7681 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     7741 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     7801 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagaattc
     7861 gatatcaagc ttatcggata acttcgtata gcatacatta tacgaagtta tcagcatagc
     7921 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     7981 ttgctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8041 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8101 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8161 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8221 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8281 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8341 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8401 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     8461 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8521 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     8581 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8641 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8701 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8761 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8821 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     8881 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     8941 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     9001 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     9061 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     9121 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9181 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9241 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     9301 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     9361 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     9421 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     9481 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     9541 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     9601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
    10021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10261 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
    10321 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
    10381 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
    10441 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
    10501 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
    10561 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
    10621 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
    10681 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
    10741 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
    10801 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
    10861 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    10921 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    10981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11101 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    11161 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11221 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11281 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11341 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11401 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11461 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11521 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11581 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11641 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11701 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11761 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11881 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11941 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    12001 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12061 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12181 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12241 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12421 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12601 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    13021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    13081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13141 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13261 tccgcgcaca tttccccgaa aagtgccacc tgac  
//
