LOCUS       Exported                3970 bp ds-DNA     circular SYN 14-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 14, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
COMMENT     >pUC19
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_binding    176..198
                     /label=LacO
     primer_bind     204..224
                     /label=M13-rev
     misc_feature    254..259
                     /label=New Feature
     misc_feature    262..278
                     /label=Chen
     misc_recomb     279..312
                     /label=LoxP
     misc_feature    279..312
                     /label=loxP
     misc_feature    313..335
                     /label=STOP+MCS
     misc_feature    353..603
                     /label=EFIas
                     /label=EFIas; EFIas; EFS; EFS; EFS
                     /note="Geneious type: promoter prokaryotic"
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro, 
                     LentiCrisprmCherryP2Apuro-improved tracer, pLentiCRISPR, 
                     pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA 
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi 
                     site in thy1.1"
                     /note="/Transferred_Similarity=94.02%"
                     /note="/X-Transferred_From=pLentiCRISPR;pLentisgRNA-Thy1.1-
                     P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    614..1213
                     /label=Puro
     misc_feature    1214..1525
                     /label=mU6
     misc_feature    1526..1527
                     /label=mU6
                     /label=mU6(1)
     misc_feature    1532..1537
                     /label=New Feature
                     /label=New Feature(1)
     primer_bind     complement(1574..1591)
                     /label=M13-fwd
     CDS             1662..1730
                     /label=LacZ alpha
     misc_feature    complement(2235..2252)
                     /label=WSF6
     CDS             2367..3026
                     /label=AmpR
     rep_origin      3124..3806
                     /label=ColE1 origin
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgaagacc tgtttaagag ctatgctgat aacttcgtat agcatacatt
      301 atacgaagtt attttttttt tttgcggccg cccagaattg acgcgtggag tgggaattgg
      361 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      421 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      481 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      541 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggttcta
      601 gagcgctgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      721 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      781 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      841 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      901 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      961 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     1021 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1081 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1141 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1201 gcccggtgcc tgagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga
     1261 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     1321 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     1381 acattttaca tgataggctt ggatttctat aagagataca aatactaaat tattatttta
     1441 aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     1501 aaatatccct tggagaaaag ccttgtttgg ggtcttcgac tctagaggat ccccgggtac
     1561 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1621 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     1681 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     1741 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     1801 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     1861 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1921 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     1981 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2041 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2101 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2161 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2221 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2281 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2341 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2401 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2461 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2521 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2581 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2641 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2701 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2761 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2821 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2881 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2941 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3001 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3061 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3121 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3841 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3901 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3961 aagcggaaga
//
