LOCUS       lenti_LSL               8302 bp ds-DNA     circular     13-NOV-2017
DEFINITION  lentiCas9-Blast.
ACCESSION   urn.local...oj-5hx36zc
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors. .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        239..815
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Hygro"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro, lentiCRISPR
                     v2 (modified), lentiCas9-Hygro, pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=CMV_immearly_promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2886..2902
                     /label=LoxP
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    772..792
                     /label=CMV_fwd_primer
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2903..2919
                     /label=LoxP(1)
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             781..1016
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak
                     - improved backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="87.71%"
                     /label=3' LTR SIN
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1017..1034
                     /gene="PBS"
                     /SECDrawAs="Region"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer;lentiGuide-Puro(modified)
                     ;pLentiCRISPR-P2A-Thy1.1;pRRL.EF1alpha.CPPT.rtTA3.IRES.Luc
                     2noSTOP.P2A.TagBFP.WPRE-PBS;pRRL.EF1alpha.rtTA3.IRES.Luc2n
                     ostop.P2A.TagBFP.(RRLM-RIL2BFP no stop) - PBS;
                     pRRL.LinkM(RRLM) -PBS;pRRL.Linker (2 AgeI
                     sites)
                     -PBS;pRRL.PPT.SF.EGFP.IRES.Puro.PTEN213158.WPRE_29-05-2012
                     -PBS;pRRL.PPT.SF.EGFP.LMO2-miR30.WPRE_Maxi_29-05-2012-PBS;
                     pRRL.PPT.SF.GFP.miR30.WPRE
                     (Schambach)
                     -PBS;pRRL.PPT.SF.rtTA3-IRES-Luc2-P2A-TagBFP-PBS;pRRL.Recip
                     ient (1 AgeI
                     site)-PBS;pRRL.SFFV.rtTA3.IRES.Luc2nostop.P2A.TagBFP.(
                     RRLM-RIL2BFPno stop)
                     -PBS;pRRL.TRE.dsRED.miR30.PGK.Venus.IRES.rtTA3(
                     lenti-TRMPVIR)-
                     PBS;pRRL.TRE.mCherry.miR30.PGK.Venus(lenti-TCMPV)
                     -PBS;pRRL.TRE3G.GFP.miRE.PGK.Puro.IRES.rtTA3(
                     lenti-T3GGmePPIR)
                     -PBS;pRRL.TRE3G.dsRED.miRE.PGK.Venus.IRES.rtTA3(
                     lenti-T3GRmePVIR) - PBS;
                     pRRL.TRE3G.empty-PBS;pRRL.cPPT.EF1alpha.rtTA3.IRES.Luc2noS
                     TOP.P2A.TagBFP.WPRE -PBS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, lentiCRISPR v2
                     (modified),lentiGuide-Puro(modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=PBS
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2886..2902
                     /label=loxP
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     1126..1170
                     /SECDrawAs="Label"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=psi
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2903..2919
                     /label=loxP(1)
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#008040"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1233..1252
                     /Sequence="GTACAGTGCAGGGGAAAGAA"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1585..1600
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGASLIKHW*"
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLV-tTRKRAB
                     (modified)"
                     /X-Transferred_Similarity="100.00%; 68.75%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=SV40_int
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1674..1972
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer; pRSI9-U6-sh-UbiC-TagRFP-2A-Puro (DECIPHER) - RRE"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=RRE
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2132..2151
                     /Sequence="GGGCAAGTTTGTGGAATTGG"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer;
                     lentiCrisprmCherryP2AneoR-improvedtracer;pLentigRNASQF;
                     pLentisgRNAThy1.1-P2A-neo +Kosak-improved backbone
                     andkilled BsmBisite in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, lentiCRISPR
                     v2(modified),lentiCrisprmCherryP2AneoR-improved
                     tracer,pLentisgRNAThy1.1-P2A-neo+Kosak - improved
                     backboneandkilled BsmBisite in thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(1)
                     /ApEinfo_label="Binding Region"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2174..2187
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW*"
                     /Transferred_From="pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="81.25%"
                     /label=SV40_int(1)
                     /ApEinfo_label="SV40_int"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2417..2439
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer;
                     lentiGuide-Puro(modified);pLentiCRISPR-P2A-Thy1.1;plKO1
                     3xLac - HRP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, lentiCRISPR v2
                     (modified),lentiGuide-Puro(modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=HRP
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2437..2560
                     /gene="PPT"
                     /SECDrawAs="Region"
                     /X-Transferred_From="RIEPTETronly; RIKPB; RIKPH; backup
                     of lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified);
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP);
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP) Copy"
                     /X-Transferred_Similarity="100.00%; 95.97%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=PPT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2440..2453
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2440..2557
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;LentiCrispr
                     mCherryP2Apuro-improved tracer;
                     backupoflentiCas9-Hygro;lentiCRISPR
                     v2(modified);lentiCas9-Blast
                     (modified)
                     ;lentiCas9-Hygro;lentiCrisprmCherryP2AneoR;lentiCrisprmChe
                     rryP2AneoR-improvedtracer;pHR-SFFV-dCas9-BFP-KRAB(
                     modified)
                     ;pLentiCRISPR;pLentiCRISPR-P2A-Thy1.1;pLentisgRNAThy1.1-P2
                     A-neo+Kosak; pLentisgRNAThy1.1-P2A-neo +Kosak
                     -improvedbackbone andkilled BsmBisite
                     inthy1.1;pLentisgRNA-Thy1.1-P2A-Neo;pLentisgRNA-Thy1.1-P2A
                     -NeoCopy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improved
                     tracer,backupoflentiCas9-Hygro,
                     lentiCRISPRv2(modified),lentiCas9-Blast
                     (modified),lentiCas9-Hygro,lentiCrisprmCherryP2AneoR,
                     lentiCrisprmCherryP2AneoR-improvedtracer,pCRISPR-P2A-puro,
                     pHR-SFFV-dCas9-BFP-KRAB(modified),pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1,pLentisgRNAThy1.1-P2A-neo+Kosak,
                     pLentisgRNAThy1.1-P2A-neo +Kosak -improvedbackbone
                     andkilled BsmBisite in thy1.1,pLentisgRNAThy1.1-P2A-neo
                     +KosakDeltaBbsILibrary Vector"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT(1)
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#fdc68a"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2441..2564
                     /Description="central polypurine tract (cPPT) and the
                     central termination sequence (CTS)"
                     /X-Transferred_From="FUW-OSKM - cPPT/CTS;
                     LentiCrisprmCherryP2Apuro-improved tracer; backup of
                     lentiCas9-Hygro; lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiCas9-Hygro;
                     lentiGuide-Puro (modified); pHR-SFFV-dCas9-BFP-KRAB
                     (modified);
                     pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE
                     (L3beta XbaI linker + SpeI linker) Mareike - cPPT/CTS;
                     pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE
                     (L3beta XbaI linker) Mareike - cPPT/CTS; pLKO.3G_Mareike
                     - cPPT/CTS; pLentiCRISPR-P2A-Thy1.1; pSIN-U6-PGK-Venus
                     (SUPV) - cPPT; pSIN-U6-TuD-anti miR-34a-PGK-Venus
                     (SUPV-miR-34a) - cPPT; pSIN-U6-TuD-anti miR150-PGK-Venus
                     (SUPV-miR150) - cPPT; pSIN-U6-TuD-anti miR21a-PGK-Venus
                     (SUPV-miR21a) - cPPT; pSIN-U6-TuD-anti miR29a-PGK-Venus
                     (SUPV-miR29a) - cPPT; pSIN-U6-TuD-anti shRen-PGK-Venus
                     (SUPV-shRen) - cPPT; pSIN-U6-TuD-anti shRen713-PGK-Venus
                     (SUPV-Ren) - cPPT"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, backup of lentiCas9-Hygro,
                     lentiCRISPRv2(modified),lentiCas9-Blast(modified),
                     lentiCas9-Hygro,lentiGuide-Puro(modified),
                     pCRISPR-P2A-puro,pHR-SFFV-dCas9-BFP-KRAB(modified),
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label="cPPT; cPPT/CTS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2441..2558
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer; backup of lentiCas9-Hygro;
                     lentiCRISPRv2(modified);lentiCas9-Blast(modified)
                     ;lentiCas9-Hygro;pHR-SFFV-dCas9-BFP-KRAB(modified)
                     ;pLentiCRISPR-P2A-Thy1.1; pWPXLd - CPPT/CTS"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, backup of lentiCas9-Hygro,
                     lentiCRISPRv2(modified),lentiCas9-Blast(modified),
                     lentiCas9-Hygro,pCRISPR-P2A-puro,pHR-SFFV-dCas9-BFP-KRAB(
                     modified),pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label="CPPT/CTS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2444..2465
                     /Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGN
                     VAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALT
                     IPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAF
                     ALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDLLFACTGSLWLDQI*;MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCAL
                     CLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCR
                     GNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVG
                     SPFGPPPRIDTVDLET*;MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVV
                     CTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVV
                     LSGKLTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQT
                     SRISLWAASPPVSPRRPD*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;LentiCrispr
                     mCherryP2Apuro-improved
                     tracer;lentiCRISPR13450;lentiCRISPR v2
                     (modified)
                     ;lentiCrisprmCherryP2AneoR;lentiCrisprmCherryP2AneoR-impro
                     vedtracer;
                     pCW-Cas9(modified);pHR-SFFV-dCas9-BFP-KRAB(modified)
                     ;pLentiCRISPR-P2A-Thy1.1;pLentisgRNAThy1.1-P2A-neo+Kosak;
                     pLentisgRNAThy1.1-P2A-neo +Kosak -improvedbackbone
                     andkilled
                     BsmBisiteinthy1.1;pLentisgRNA-Thy1.1-P2A-Neo;pLentisgRNA-T
                     hy1.1-P2A-NeoCopy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDLLFACTGSLWLDQI*;MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWC
                     ALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWC
                     CRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRR
                     VGSPFGPPPRVDFKTNDLQGSCRS*;MPLYHAIASRMAFIFSSLYKSWLLSLYEELWP
                     VVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARL
                     LGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLP
                     RLRLRPQTSRISLWAASPPVSPRRPD*"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improvedtracer,lentiCRISPR13450,
                     
                     lentiCas9-Blast(modified),lentiCrisprmCherryP2AneoR,
                     lentiCrisprmCherryP2AneoR-improvedtracer,
                     pCW-Cas9(modified),pHR-SFFV-dCas9-BFP-KRAB(modified),
                     pLentiCRISPR-P2A-Thy1.1,
                     pLentisgRNAThy1.1-P2A-neo+Kosak,pLentisgRNA
                     Thy1.1-P2A-neo +Kosak -improvedbackboneandkilled BsmBi
                     site in thy1.1"
                     /Transferred_Similarity="86.36%"
                     /label=U3PPT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2444..2459
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
                     /label=cPPT(2)
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2467..2486
                     /Sequence="GTACAGTGCAGGGGAAAGAA"
                     /X-Transferred_From="185_primer_bind12;_185_primer_bind12;
                     backup of
                     lentiCas9-Hygro;lentiCas9-Blast(modified)
                     ;lentiCas9-Hygro;pHR-SFFV-dCas9-BFP-KRAB(modified)
                     ;pLentisgRNAThy1.1-P2A-neo +Kosak;primer_bind12"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, lentiGuide-Puro (modified),
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(2)
                     /ApEinfo_label="Binding Region"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2479..2522)
                     /created_by="User"
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=R1 CRISPR
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2479..2522)
                     /Sequence="CTTTAGTTTGTATGTCTGTTGCTATTATGTCTACTATTCTTTCC"
                     /X-Transferred_From="285_R1 Crispr; R1 Crispr; _285_R1
                     Crispr; backup of lentiCas9-Hygro; lentiCas9-Blast
                     (modified); lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB
                     (modified); pLentisgRNA Thy1.1-P2A-neo +Kosak"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pCRISPR-P2A-puro,
                     pHR-SFFV-dCas9-BFP-KRAB (modified), pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(3)
                     /ApEinfo_label="Binding Region"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2601..2840
                     /X-Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo;pLentisgRN
                     A-Thy1.1-P2A-Neo Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /label=hU6_promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2841..2868
                     /X-Transferred_From="final_retro_CRIS"
                     /X-Transferred_Similarity="72.01%"
                     /modified_by="User"
                     /Transferred_From="pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: concatenated sequence"
                     /label=Filler
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 2869..2885
                     /created_by="User"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer, pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=improved tracer
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2982..2991
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;lentiCrispr
                     mCherryP2AneoR; pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro, lentiCRISPR
                     v2 (modified), lentiCrispr mCherryP2AneoR,
                     pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=U6 terminator
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2994..3582
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak
                     - improved backbone andkilled BsmBi site in thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=WPRE
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3766..3779
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=cPPT(3)
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3770..3791
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=U3PPT(1)
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3770..3785
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=cPPT(4)
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3786..3839
                     /invitrogen="1410000"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer;lentiGuide-Puro(modified);plenti6gw_u6laminshrna_verB
                     -delta U3"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, lentiGuide-Puro (modified),pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="81.48%"
                     /label=delta U3
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3787..3839
                     /Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGN
                     VAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALT
                     IPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAF
                     ALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQ
                     RRQDLLFACTGSLWLDQI*;MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCAL
                     CLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCR
                     GNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVG
                     SPFGPPPRVDFKTNDLQGSCRS*;MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVV
                     RQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLG
                     TDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRL
                     RLRPQTSRISLWAASPPVSPRRPD*"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro;LentiCrispr
                     mCherryP2Apuro-improved
                     tracer;lentiCRISPR13450;lentiCRISPR v2
                     (modified)
                     ;lentiCrisprmCherryP2AneoR;lentiCrisprmCherryP2AneoR-impro
                     vedtracer;lentiGuide-Puro
                     (modified);pCW-Cas9(modified);pHR-SFFV-dCas9-BFP-KRAB(
                     modified)
                     ;pLentiCRISPR-P2A-Thy1.1;pLentisgRNAThy1.1-P2A-neo+Kosak;
                     pLentisgRNAThy1.1-P2A-neo +Kosak -improvedbackbone
                     andkilled
                     BsmBisiteinthy1.1;pLentisgRNA-Thy1.1-P2A-Neo;pLentisgRNA-T
                     hy1.1-P2A-NeoCopy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Transferred_Translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLS
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDLLFACTGSLWLDQI*;MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWC
                     ALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWC
                     CRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRR
                     VGSPFGPPPRVDFKTNDLQGSCRS*;MPLYHAIASRMAFIFSSLYKSWLLSLYEELWP
                     VVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARL
                     LGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLP
                     RLRLRPQTSRISLWAASPPVSPRRPD*"
                     /Transferred_From="LentiCrisprmCherryP2Apuro,
                     LentiCrisprmCherryP2Apuro-improvedtracer,lentiCRISPR13450,
                     
                     lentiCrisprmCherryP2AneoR,
                     lentiCrisprmCherryP2AneoR-improvedtracer,lentiGuide-Puro(
                     modified),
                     pCW-Cas9(modified),pHR-SFFV-dCas9-BFP-KRAB(modified),
                     pLentiCRISPR-P2A-Thy1.1,
                     pLentisgRNAThy1.1-P2A-neo+Kosak,pLentisgRNA
                     Thy1.1-P2A-neo +Kosak -improvedbackboneandkilled BsmBi
                     site in thy1.1"
                     /Transferred_Similarity="81.13%"
                     /label=delta_U3
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4053..4288
                     /X-Transferred_From="lentiGuide-Puro (modified);
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak
                     - improved backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR v2 (modified),
                     lentiGuide-Puro (modified), pLentiCRISPR,
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="86.44%"
                     /label=3' LTR SIN(1)
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             4295..4526
                     /Transferred_From="PX335 D10N PV"
                     /Transferred_Similarity="97.41%"
                     /label=bGH polyA
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4313..4330)
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=BGH_rev_primer
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4508..4534)
                     /Sequence="GCGGCCGCTCCCCAGCATGCCTGCTAT"
                     /X-Transferred_From="NotI bGHR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="PX330"
                     /Transferred_Similarity="77.78%"
                     /label=Binding Region(4)
                     /ApEinfo_label="Binding Region"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4589..5044
                     /X-Transferred_From="pLentiCRISPR;pLentiCRISPR-P2A-Thy1.1;
                     pLentisgRNA
                     Thy1.1-P2A-neo+Kosak;pLentisgRNAThy1.1-P2A-neo +Kosak -
                     improvedbackboneandkilled BsmBisite
                     inthy1.1;pLentisgRNA-Thy1.1-P2A-Neo;pLentisgRNA-Thy1.1-P2A
                     -NeoCopy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiGuide-Puro (modified),
                     pLentiCRISPR, pLentisgRNA Thy1.1-P2A-neo +Kosak,
                     pLentisgRNA Thy1.1-P2A-neo +Kosak - improved backbone
                     andkilled BsmBi site in thy1.1"
                     /Transferred_Similarity="95.18%"
                     /label=F1 ori
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5044..5312
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=SV40_promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        5406..5473
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=EM7_promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            5474..5845
                     /gene="bleo"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLET*"
                     /label=bleo
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polya_signal    5978..6108
                     /X-Transferred_From="backup of lentiCas9-Hygro;
                     lentiCRISPR v2 (modified); lentiCas9-Blast (modified);
                     lentiCas9-Hygro; lentiGuide-Puro (modified);
                     pHR-SFFV-dCas9-BFP-KRAB (modified); pLentiCRISPR;
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNA Thy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - improved
                     backbone andkilled BsmBi site in thy1.1;
                     pLentisgRNA-Thy1.1-P2A-Neo; pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro, lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified),
                     lentiCas9-Hygro, lentiGuide-Puro (modified),
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified),
                     pLentiCRISPR, pLentiCRISPR-P2A-Thy1.1, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak
                     - improved backbone andkilled BsmBi site in thy1.1,
                     pLentisgRNA Thy1.1-P2A-neo +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=SV40 polyadenylation signal
                     /ApEinfo_fwdcolor="#6ecff6"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6138..6367
                     /Description="lac operon sequence"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer; backup of lentiCas9-Hygro;
                     lentiCRISPRv2(modified);lentiCas9-Blast(modified)
                     ;lentiCas9-Hygro;lentiGuide-Puro
                     (modified);pGEM4z(Promega)@1 -Misc_Feature_4; pGEM4z-Bcl2
                     (G145A)@1-Misc_Feature_4;pLentiCRISPR-P2A-Thy1.1"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, backup of lentiCas9-Hygro,
                     lentiCRISPRv2(modified),lentiCas9-Blast(modified),
                     lentiCas9-Hygro,lentiGuide-Puro(modified),
                     pCRISPR-P2A-puro,pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="100.00%"
                     /label=Misc_Feature_4
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6366..6380)
                     /Transferred_Translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKV
                     GIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
                     LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEE
                     DKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGH
                     FLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENL
                     IAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIG
                     DQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
                     GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLY
                     EYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE
                     CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREM
                     IEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFA
                     NRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDEL
                     VKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQ
                     LQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN
                     RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQL
                     VETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREIN
                     NYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYF
                     FYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIV
                     KKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGK
                     SKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRK
                     RMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEI
                     IEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYF
                     DTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKKK*
                     /X-Transferred_Translation=%22MDYKDHDGDYKDHDIDYKDDDDKMAPKK
                     KRKVGIHGVPAADKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNL
                     IGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRR
                     LENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLL
                     AQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKA
                     LVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNR
                     EDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVG
                     PLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKH
                     SLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYF
                     KKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE
                     DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKS
                     DGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKV
                     VDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPV
                     ENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTR
                     SDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFI
                     KRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKV
                     REINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKAT
                     AKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQ
                     VNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELE
                     NGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHY
                     LDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA
                     FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAK
                     KKK*"
                     /X-Transferred_From="lentiCRISPR 13450; pCW-Cas9
                     (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCRISPR 13450, pCW-Cas9
                     (modified), pLentiCRISPR-P2A-Thy1.1"
                     /Transferred_Similarity="80.00%"
                     /label=NLS
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6367..6430)
                     /Description=" L-strand Y effector site ; L-strand Y
                     effector site"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=L_Y_EFF
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6372..6383)
                     /product="3' stem, pBR pas(L)"
                     /gene="pas'"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=(pas')
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6415..6425)
                     /product="5' stem, pBR primosome ass'y site (L)"
                     /gene="pas"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=(pas)
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6491..7164
                     /Description="derived originally from a ColE1-like
                     plasmid, pMB1"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improvedtra
                     cer; backup of lentiCas9-Hygro;
                     lentiCRISPRv2(modified);lentiCas9-Hygro;
                     pIBHistoneH2B-mCherry -pUCori"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LentiCrisprmCherryP2Apuro-improvedtrace
                     r, backup of lentiCas9-Hygro,
                     lentiCRISPRv2(modified),lentiCas9-Hygro, pCRISPR-P2A-puro"
                     /Transferred_Similarity="100.00%"
                     /label=pUC ori
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     complement(7162..7199)
                     /Description=" Tn3 inverted repeat "
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /label=TN3_INV_RPT
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             7309..8169
                     /X-Transferred_From="Reporter Puro_P2A-tGFP"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="TPGPB-Cas9(PX330)"
                     /Transferred_Similarity="99.77%"
                     /label=Amp
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(8170..8268)
                     /invitrogen="2330000"
                     /X-Transferred_From="LUPG FKBP tTA P2A; backup of
                     lentiCas9-Hygro; lentiCas9-Blast (modified);
                     lentiCas9-Hygro; pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pCRISPR-P2A-puro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter uukaryotic"
                     /label=bla promoter
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 2920..2990
                     /created_by="User"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer, pLentisgRNA Thy1.1-P2A-neo +Kosak, pLentisgRNA
                     Thy1.1-P2A-neo +Kosak - improved backbone andkilled BsmBi
                     site in thy1.1, pLentisgRNA Thy1.1-P2A-neo
                     +KosakDeltaBbsI Library Vector"
                     /Transferred_Similarity="100.00%"
                     /label=improved tracer(1)
                     /ApEinfo_label="improved tracer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2920..2991
                     /label=to add
                     /ApEinfo_fwdcolor="#37f807"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..8302
                     /dnas_title="lenti_LSL"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta tttcccatga ttccttcata tttgcatata cgatacaagg
     2641 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     2701 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     2761 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2821 tgtggaaagg acgaaacacc ggagacgatc agtatacgta acgtctctgt ttaagagcta
     2881 tgctgataac ttcgtatagc atacattata cgaagttatc agcatagcaa gtttaaataa
     2941 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gctaatcaac
     3001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3361 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     3601 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     3661 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     3721 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3781 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     3841 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     3901 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     3961 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     4021 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     4081 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4141 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4201 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4261 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     4321 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4381 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4441 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4501 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4561 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4621 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4681 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4741 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4801 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4861 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4921 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4981 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5041 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5101 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5161 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5221 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5281 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5341 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5401 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     5461 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     5521 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     5581 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     5641 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     5701 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     5761 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     5821 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     5881 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5941 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6121 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6181 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6241 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6301 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6361 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6421 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6481 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6961 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7021 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7081 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7141 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7201 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7261 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7321 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7381 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7441 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7501 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7561 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7621 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7681 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7741 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7801 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7861 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7921 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7981 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8041 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8101 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8161 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8221 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8281 tccccgaaaa gtgccacctg ac
//

