LOCUS       CKII-NPTX1-pHluorin    11294 bp ds-DNA     circular     07-MAR-2024
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: Camkii NPX1
            phluorin_assembledseq.gb synthetic DNA NEBuilder-generated oligos
            (UPPERCASE = gene-specific, lowercase = overlap)
            CamKII_LGI1_pHluorin_fwd: ccagatcaacAGCGGCTCTACTAGTGGTG
            CamKII_LGI1_pHluorin_fwd 3'Tm: 66.3  3'Ta: 62.9
            CamKII_LGI1_pHluorin_rev: gcattggtaaCTGTCAGACCAAGTTTACTCATATATAC
            CamKII_LGI1_pHluorin_rev 3'Tm: 61.9  3'Ta: 62.9
            CamKII_LGI1_pHluorin_fwd:
            ggtctgacagTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGC
            CamKII_LGI1_pHluorin_fwd 3'Tm: 73.2  3'Ta: 72.0
            CamKII_LGI1_pHluorin_rev: cggccagcatGGTGGCGGATCCCCCGCT
            CamKII_LGI1_pHluorin_rev 3'Tm: 77.1  3'Ta: 72.0 insert_fwd:
            atccgccaccATGCTGGCCGGCCGCGCC insert_fwd 3'Tm: 81.9  3'Ta: 72.0
            insert_rev: tagagccgctGTTGATCTGGCGGCAAGCCTCGAATGTCC insert_rev
            3'Tm: 77.1  3'Ta: 72.0
FEATURES             Location/Qualifiers
     promoter        6..1206
                     /label="CamKII promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(645..650)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(1236..1263)
                     /label="gene-specific primer: GGTGGCGGATCCCCCGCT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     1244..1271
                     /label="gene-specific primer: ATGCTGGCCGGCCGCGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1248..1253
                     /label="kozak"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1254..2549
                     /label="rat NP1 WT FL"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 transl_table: 1 translation: MLAGRAARTCALLALCLLGSRAQDFGPTRFICTSVPVDADMCAASVAAGGAEELRSNVLQLRETVLQQKETILSQKETIRELTTKLGRCESQSTLDAGPGEARSGGGRKQPGSGKNTMGDLSRTPASETLSQLGQTLQSLKTRLENLEQYSRLNSSSQTNSLKDLLQSKIDDLERQVLSRVNTLEEGKGGPKNDTEERAKIESALTSLHQRISELEKGQKDNRPGDKFQLTFPLRTNYMYAKVKKSLPEMYAFTVCMWLKSSAAPGVGTPFSYAVPGQANELVLIEWGNNPMEILINDKVAKLPFVINDGKWHHICVTWTTRDGVWEAYQDGTQGGNGENLAPYHPIKPQGVLVLGQEQDTLGGGFDATQAFVGELAHFNIWDRKLTPGEVYNLATCSSKALSGNVIAWAESQIEIFGGATKWTFEACRQIN"
     CDS             1254..3302
                     /label="Translation 1254-3302"
                     /translation="MLAGRAARTCALLALCLLGSRAQDFGPTRFICTSVPVDADMCAASVAAGGAEELRSNVLQLRETVLQQKETILSQKETIRELTTKLGRCESQSTLDAGPGEARSGGGRKQPGSGKNTMGDLSRTPASETLSQLGQTLQSLKTRLENLEQYSRLNSSSQTNSLKDLLQSKIDDLERQVLSRVNTLEEGKGGPKNDTEERAKIESALTSLHQRISELEKGQKDNRPGDKFQLTFPLRTNYMYAKVKKSLPEMYAFTVCMWLKSSAAPGVGTPFSYAVPGQANELVLIEWGNNPMEILINDKVAKLPFVINDGKWHHICVTWTTRDGVWEAYQDGTQGGNGENLAPYHPIKPQGVLVLGQEQDTLGGGFDATQAFVGELAHFNIWDRKLTPGEVYNLATCSSKALSGNVIAWAESQIEIFGGATKWTFEACRQINSGSTSGGSGGTGGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"
     primer_bind     complement(2521..2559)
                     /label="gene-specific primer: GTTGATCTGGCGGCAAGCCTCGAATGTCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     2540..2568
                     /label="gene-specific primer: AGCGGCTCTACTAGTGGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2550..2585
                     /label="linker"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2586..3302
                     /label="pHluorin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        2607..2612
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3333..3921
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3384..3390
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(3804..3815)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3924..3940)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     LTR             4446..4626
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4658..4882
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      4928..5356
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4945..5251
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        5370..5699
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      5550..5685
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        5747..5794
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5813..6187
                     /label="BleoR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    6317..6438
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6483..6503)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(6487..6503)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     misc_binding    complement(6509..6531)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    6511..6527
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(6535..6541)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(6535..6565)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(6542..6559)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(6560..6565)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    6580..6601
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(6871..7553)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(7302..7307)
                     /label="kozak"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(7620..7657)
                     /label="gene-specific primer: CTGTCAGACCAAGTTTACTCATATATAC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     7638..7683
                     /label="gene-specific primer: TTACCAATGCTTAATCAGTGAGGCACCTATCTCAGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(7648..8439)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(8440..8508)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(8509..8613)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     enhancer        8879..9258
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        8955..9258
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        9260..9458
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             9476..9656
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    9703..9828
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    10321..10554
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             10739..10783
                     /label="gp41 peptide"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: recognized by the 2H10 single-chain llama nanobody product: antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013) transl_table: 1 translation: KNEQELLELDKWASL"
     misc_feature    11081..11198
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 agaaaatgag atgggggtgc agagagcttc ctcagtgacc tgcccagggt cacatcagaa
       61 atgtcagagc tagaacttga actcagatta ctaatcttaa attccatgcc ttgggggcat
      121 gcaagtacga tatacagaag gagtgaactc attagggcag atgaccaatg agtttaggaa
      181 agaagagtcc agggcagggt acatctacac cacccgccca gccctgggtg agtccagcca
      241 cgttcacctc attatagttg cctctctcca gtcctacctt gacgggaagc acaagcagaa
      301 actgggacag gagccccagg agaccaaatc ttcatggtcc ctctgggagg atgggtgggg
      361 agagctgtgg cagaggcctc aggaggggcc ctgctgctca gtggtgacag ataggggtga
      421 gaaagcagac agagtcattc cgtcagcatt ctgggtctgt ttggtacttc ttctcacgct
      481 aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg agagctggaa agaaacaagg
      541 acagagacag aggccaagtc aaccagacca attcccagag gaagcaaaga aaccattaca
      601 gagactacaa gggggaaggg aaggagagat gaattagctt cccctgtaaa ccttagaacc
      661 cagctgttgc cagggcaacg gggcaatacc tgtctcttca gaggagatga agttgccagg
      721 gtaactacat cctgtctttc tcaaggacca tcccagaatg tggcacccac tagccgttac
      781 catagcaact gcctctttgc cccacttaat cccatcccgt ctgttaaaag ggccctatag
      841 ttggaggtgg gggaggtagg aagagcgatg atcacttgtg gactaagttt gttcacatcc
      901 ccttctccaa ccccctcagt acatcaccct gggagaacaa ggtccacttg cttctgggcc
      961 cacacagtcc tgcagtattg tgtatataag gccagggcaa cggaggagca ggttttgaag
     1021 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
     1081 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
     1141 gtgccctgct cagaagcccc aagctcgtca atcaagctgg ttctccattt gcactcagga
     1201 gcacgggcag gcgagtggcc cctagttctg ggggcagcgg gggatccgcc accatgctgg
     1261 ccggccgcgc cgcacgcacc tgtgcgctgc tcgccctctg cctcctgggc agtcgggccc
     1321 aggatttcgg gccgacccgc ttcatctgca cttcggtgcc ggtggacgcc gacatgtgtg
     1381 ccgcgtccgt ggccgcgggc ggcgcggagg agcttcggag caatgtgctg cagctccggg
     1441 agaccgtgct gcagcagaag gagaccatcc tcagccagaa ggagaccatc agggagctga
     1501 ccaccaagct tggccgctgc gagagccaga gcaccctgga cgcgggtccg ggcgaggcca
     1561 ggtcgggcgg cggccgcaag cagcctggct cgggcaagaa tacgatgggc gacctgtccc
     1621 ggactccggc ctcggagacg ctcagccaac tcgggcaaac tttgcaatcc ctcaaaaccc
     1681 gactggagaa cctcgagcag tacagccgcc tcaattcttc cagccaaacc aacagcctca
     1741 aggatctgct gcagagcaag atcgatgacc tggagcggca ggtgctgtct cgagtgaaca
     1801 ctctggagga gggaaaaggg ggccccaaga acgacacaga ggaaagggcc aagatcgaga
     1861 gcgccttgac ctccctacac cagcggatca gcgagctgga gaaaggtcag aaagacaatc
     1921 gacccggaga caagtttcag ctgacattcc cactgcggac caactacatg tatgccaagg
     1981 tgaagaagag cctgccggag atgtacgcct tcactgtgtg catgtggctc aagtccagcg
     2041 cggcacctgg agtgggcacg cccttctcct atgctgtacc tgggcaggcc aacgagctgg
     2101 tcctcatcga gtggggcaac aaccccatgg agatactcat taacgacaag gtggccaagc
     2161 tgccctttgt aatcaacgac ggcaagtggc accacatatg cgttacctgg accacccggg
     2221 acggggtctg ggaggcctat caggatggca cccagggagg caatggagag aacctggcac
     2281 cctatcaccc tatcaaacca cagggtgtgc tggtgctagg ccaggagcag gacactctag
     2341 gcggagggtt tgatgccacc caagcgtttg tgggtgagct agcccatttc aacatctggg
     2401 accgcaagct gacccctggg gaggtgtaca acttggccac ctgcagcagc aaggccctct
     2461 ctggcaatgt catcgcctgg gctgagtccc agatcgagat ctttggagga gccaccaagt
     2521 ggacattcga ggcttgccgc cagatcaaca gcggctctac tagtggtgga tctggtggaA
     2581 CCGGTggctc caaaggcgag gaactgttta caggcgtggt gcctatcctg gtcgagctgg
     2641 atggggatgt gaacggccac aagttctccg tgtctggcga aggcgaaggg gacgccacat
     2701 acggaaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg ccttggccta
     2761 cactggtcac cacactgaca tacggcgtgc agtgcttcag cagatacccc gaccatatga
     2821 agaggcacga cttcttcaag agcgccatgc ctgagggcta cgtgcaagag aggaccatct
     2881 tctttaagga cgacggcaac tacaagacca gggccgaagt gaagttcgag ggcgacaccc
     2941 tggtcaacag gatcgagctg aagggaatcg acttcaaaga ggacggcaac atcctgggac
     3001 acaagttgga gtacaactac aacgatcacc aggtctacat catggccgat aagcagaaga
     3061 acggcatcaa ggccaacttc aagatccggc acaacatcga ggacggcgga gtgcagctgg
     3121 ccgatcacta tcagcagaac acccctatcg gcgacggacc tgtgctgctg cctgataacc
     3181 actacctgtt taccaccagc acactgagca aggaccccaa cgagaagagg gaccacatgg
     3241 tgctgttgga gttcgtgaca gccgccggaa tcacacacgg aatggacgag ctgtacaaat
     3301 gagatatcga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     3361 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3421 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3481 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3541 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3601 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3661 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3721 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     3781 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     3841 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     3901 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     3961 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     4021 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     4081 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4141 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     4201 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     4261 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     4321 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     4381 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     4441 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4501 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4561 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4621 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4681 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4741 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4801 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4861 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4921 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4981 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5041 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5101 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5161 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5221 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5281 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5341 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5401 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5521 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5581 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5641 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5701 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     5761 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     5821 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     5881 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     5941 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     6001 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     6061 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     6121 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     6181 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6241 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6301 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6421 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6481 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6541 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6661 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6721 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6781 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6841 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6901 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6961 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7021 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7081 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7141 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7201 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7261 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7321 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7381 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7441 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7501 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7621 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8521 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8641 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     8701 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     8761 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     8821 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     8881 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8941 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9001 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9061 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9121 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9181 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9241 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     9301 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     9361 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     9421 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     9481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     9661 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     9721 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     9781 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     9841 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9901 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9961 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    10021 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    10081 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    10141 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
    10201 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    10261 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    10321 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    10381 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10441 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    10501 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10561 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10621 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10681 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10741 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10801 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10861 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10921 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    10981 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
    11041 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    11101 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    11161 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    11221 gagatccagt ttggttaatt aacattatgg ccttaggtca cttcatctcc atggggttct
    11281 tcttctgatt ttct
//