LOCUS       pAAV-hSyn-FLEX-H        7275 bp DNA     circular SYN 06-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=AAV ITR
                     /note="color: #f8d3a9"
     promoter        158..605
                     /label=hSyn promoter
                     /note="color: #c6c9d1"
     misc_feature    612..621
                     /label=Kozak Sequence
                     /note="color: #b4abac"
     regulatory      612..621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    618..620
                     /label=Start Codon
                     /note="color: #ff00ff"
     protein_bind    627..660
                     /label=loxP
                     /note="color: #31849b"
     protein_bind    711..744
                     /label=lox2272
                     /note="color: #31849b"
     misc_feature    751..2325
                     /label=oG
                     /note="color: #339966"
     misc_feature    889..948
                     /label=Transmembrane
                     /note="color: #b4abac"
     CDS             complement(2326..2382)
                     /label=P2A
                     /note="color: #ff9ccd; direction: LEFT"
     CDS             complement(2326..2382)
                     /codon_start=1
                     /label=Translation 2382-2326
                     /note="color: #993366"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2398..2484)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYAGYPYDVPDYAGYPYDVPDYA"
     CDS             complement(2398..2424)
                     /label=HA
                     /note="color: #ff9ccd; direction: LEFT"
     CDS             complement(2428..2454)
                     /label=HA
                     /note="color: #ff9ccd; direction: LEFT"
     CDS             complement(2458..2484)
                     /label=HA
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(2485..3195)
                     /label=mTAGBFP2
                     /note="color: #3366ff; direction: LEFT"
     regulatory      3192..3201
                     /label=Kozak sequence
                     /note="color: #b4abac"
     CDS             complement(3214..3591)
                     /label=H2B
                     /note="color: #993366; direction: LEFT"
     protein_bind    complement(3597..3630)
                     /label=loxP
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(3681..3714)
                     /label=lox2272
                     /note="color: #31849b; direction: LEFT"
     misc_feature    3716..4304
                     /label=WPRE
                     /note="color: #c6c9d1"
     CDS             complement(4187..4198)
                     /label=Factor Xa site
                     /note="color: #ff9ccd; direction: LEFT"
     polyA_signal    4312..4536
                     /label=bGH poly(A) signal
                     /note="color: #b4abac"
     repeat_region   4538..4678
                     /label=AAV2 ITR
                     /note="color: #f8d3a9"
     rep_origin      4753..5208
                     /label=f1 ori
                     /note="color: #ffef86"
     promoter        5490..5594
                     /label=AmpR promoter
                     /note="color: #c6c9d1"
     CDS             5595..6455
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5595 .. 5663 / #ccffcc / signal sequence
                        2: 5664 .. 6455 / #ccffcc
                     Cleavage site after base 5663"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5595..5663
                     /label=AmpR
                     /note="color: #b7e6d7; direction: RIGHT"
     CDS             5664..6455
                     /label=AmpR
                     /note="color: #b7e6d7; direction: RIGHT"
     rep_origin      6626..7214
                     /label=ori
                     /note="color: #ffef86"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gctagactgc agagggccct gcgtatgagt gcaagtgggt tttaggacca
      181 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc
      241 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc
      301 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg
      361 gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc
      421 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac
      481 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg
      541 gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag
      601 cgcagtcagc cgccaccatg gttaatataa cttcgtataa tgtatgctat acgaagttat
      661 gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta ataacttcgt
      721 ataaagtatc ctatacgaag ttatggtacc ttagagccgt gtctcgcccc cagatttatg
      781 actttcccaa ctgctgatga ttttaccgct ctggggtgtg acagacactt ctctaccggt
      841 ccccctcaga ttgtgctgag ttggctcgga gcggttcact cgtcgccagc aggtcatcag
      901 gaaaatgatc agcatgagag cagtcagtgc tccagcggag agcagcacgt acttgcccca
      961 attaggcagt ccgagatcga cgccactgat ccgttcgtgc acgtcgggca ggtggacctc
     1021 cacaaaatct tcggcctcgt cgccgttttt gaacactgtg cttgggtctg cgagaggatg
     1081 catcagggga atgacgcttg acacgagcag ttccatgtgc tgctggagca gactagactg
     1141 catctcgggg atcagcacgt taccatctgg ccccagaatg ataccattaa agaagacccc
     1201 gttcacatgt gggtgacatc gacccccgac tcgcaggcat cccttactag gaatgatttc
     1261 attccaggtt ctcacagatt tataatgggc gtctgcctcc atcagtgtct tgttaaagat
     1321 ggtgtatgct tttccgaagc cagggacgag cttcctcagg tggctcagcc tcctgaagct
     1381 cacgctcttg gtagtcataa ttgactccag agcatcgaga cattcctccc ttttcttcac
     1441 gagttcctcg accaccagat gttcgatctc atcgcttcgg aagtcgtgca gattcacgag
     1501 ctgcccaggg gggcaccact tagtctcgtt agatgtctgc atagcgaccc aggttccgtc
     1561 catcagccgg agtcccagca cgccacagag tttcagcttg caggcacctt tgaggctctt
     1621 atacagccct ctttcatcca caaatccaca agtctcgctg ccttttgagg ccctcttgcc
     1681 cctgctgttg gtgaagatgt cgcaggacat ccccagcctt ggattctcag gcatccagat
     1741 tgtataatca tggttggtgg aacagtaagt ggagctgacg gccactccgg agcagtttcc
     1801 gccgggaaac actggactgt ggagagatcg gtcgtagggg tccaggtcgg cgacagatgg
     1861 ggaaatgatg accaggctct cttttgtggt cttcacagtg cgcagccaat gatagtctgg
     1921 gtaaggattg tgcaggcttt cctcatagcg ggggtcgcca gccatcttcc agttgtaagc
     1981 tgcccgacag gcgtctggtg ttggccgaaa gtgttttctc ttgaaagttg tggtgacata
     2041 tcccacaaaa tttgtgtagg tttctgcctc ggtgaccaca ccagtgcatg tgaacccgtt
     2101 catcttaata gcagagatat agcccacttt cagctccatg taggagaatc cgctcaggtt
     2161 ggtacagcct tcgtcctcga ccacgagatt gttagggcat gacagatggt gaatatcgat
     2221 aggactccag gggcccagct tgtcggggat agtgtagata gggaatttac caaagcagag
     2281 tgggaagacg agcagaggga cgaagaggag agcctgtggg accatgggtc cagggttctc
     2341 ctccacgtct ccagcctgct tcagcaggct gaagttagta gctccggatc ctccgccagc
     2401 gtaatctgga acgtcatatg gatatcctgc atagtccggg acgtcatagg gatagcccgc
     2461 atagtcagga acatcgtatg ggtagttgag cttatgaccc aacttactag gcaaatcaca
     2521 gtagcgggcg acggcgactt cgtgctgctc gacgtaggtt tcgttgttag cttccttaat
     2581 gcgctccagc ctatagtcca cgtagtacac accgggcatc ttcaggttct tcgctggctt
     2641 cttactacgg tacgtagtct tggcattggc tataaggtgc gaaccgccca ccagcttcag
     2701 agccatatcg ttgcgacctt caagaccgcc atcggcgggg tacagcgtct ccgtaaaggc
     2761 ctcccatccg agcgtctttt tctgcataac cgggccgttc gaggtaaagt tcacgccgcg
     2821 tatcttcacg ttgtaaatca agcaaccatc ctgcaggcta gtatcttggg tggcagtcag
     2881 aactccgccg tcctcatagg tagtaacgcg ttcccatgtg aagccttcag ggaaggattg
     2941 cttgaagaaa tctggtattc cctgagtatg attgatgaat gtcttgctgc catagaggaa
     3001 actggtggcc aggatatcga atgcaaatgg cagtggacct ccctcgacaa ctttaatacg
     3061 catggtctgt gtgccctcgt agggctttcc ctcaccctcg ctggtgcact taaagtggtg
     3121 gttgtcgacg gttccctcca tatacagctt catgtgcata ttctctttga tcagctcctc
     3181 tcccttcgaa accatggtgg cgaccggtgg atccttagcg ctggtgtact tggtgatggc
     3241 cttagtaccc tcggacacgg cgtgcttggc caactcccca ggcagcagca ggcgcacggc
     3301 cgtctggatc tccctggagg tgatggtcga gcgcttgttg taatgcgcca ggcgggaagc
     3361 ctcacctgcg atgcgctcga aaatgtcgtt cacaaacgaa ttcatgatgc ccatggcctt
     3421 ggacgaaatg ccggtgtcag ggtggacctg cttcagaacc ttgtacacat agatggaata
     3481 gctctccttg cggctgcgct tgcgcttctt gccgcctttc ttctgcgcct tagtcaccgc
     3541 cttcttggag ccctttttcg gggcgggagc agacttcgct ggctctggca tacctaataa
     3601 cttcgtatag catacattat acgaagttat ttgccttaac ccagaaatta tcactgttat
     3661 tctttagaat ggtgcaaaga ataacttcgt ataggatact ttatacgaag ttatgaatca
     3721 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3781 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3841 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3901 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3961 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4021 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4081 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4141 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4201 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4261 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggat cctgtgcctt
     4321 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4381 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4441 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4501 atagcaggca tgctggggat gcggtgggct ctatggcagg aacccctagt gatggagttg
     4561 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4621 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     4681 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     4741 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4801 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4861 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4921 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4981 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5041 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     5101 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5161 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     5221 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5281 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5341 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5401 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5461 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5521 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5581 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5641 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5701 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5761 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5821 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5881 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5941 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6001 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6061 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6121 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6181 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6241 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6301 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6361 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6421 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6481 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6541 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6601 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6661 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6721 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6781 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6841 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6901 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6961 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7021 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7081 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7141 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7201 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7261 cttttgctca catgt
//
